Experiment: MoLS4 with L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ade and DVU0442 are separated by 446 nucleotides DVU0442 and DVU0443 are separated by 212 nucleotides DVU0443 and DVU0444 are separated by 7 nucleotides
DVU0441: ade - adenine deaminase (TIGR), at 497,613 to 499,340
ade
DVU0442: DVU0442 - phoH-related protein (TIGR), at 499,787 to 500,983
DVU0442
DVU0443: DVU0443 - exonuclease, putative (Keith Keller), at 501,196 to 501,930
DVU0443
DVU0444: DVU0444 - CBS domain protein (TIGR), at 501,938 to 504,160
DVU0444
Position (kb)
499
500
501 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 498.812 kb on + strand, within ade at 498.839 kb on + strand, within ade at 498.866 kb on - strand, within ade at 498.866 kb on - strand, within ade at 498.906 kb on + strand, within ade at 498.928 kb on + strand, within ade at 498.936 kb on - strand, within ade at 499.229 kb on - strand at 499.305 kb on - strand at 499.336 kb on + strand at 499.344 kb on - strand at 499.344 kb on - strand at 499.394 kb on + strand at 499.402 kb on - strand at 499.402 kb on - strand at 499.402 kb on - strand at 499.402 kb on - strand at 499.489 kb on + strand at 499.497 kb on - strand at 499.497 kb on - strand at 499.497 kb on - strand at 499.509 kb on + strand at 499.534 kb on + strand at 499.537 kb on - strand at 499.547 kb on - strand at 499.565 kb on - strand at 499.580 kb on + strand at 499.730 kb on + strand at 499.738 kb on - strand at 499.740 kb on - strand at 499.767 kb on + strand at 499.880 kb on - strand at 499.880 kb on - strand at 499.895 kb on + strand at 499.895 kb on + strand at 499.971 kb on + strand, within DVU0442 at 499.972 kb on - strand, within DVU0442 at 499.985 kb on + strand, within DVU0442 at 500.018 kb on + strand, within DVU0442 at 500.026 kb on - strand, within DVU0442 at 500.026 kb on - strand, within DVU0442 at 500.026 kb on - strand, within DVU0442 at 500.084 kb on - strand, within DVU0442 at 500.106 kb on - strand, within DVU0442 at 500.133 kb on + strand, within DVU0442 at 500.183 kb on + strand, within DVU0442 at 500.191 kb on - strand, within DVU0442 at 500.199 kb on + strand, within DVU0442 at 500.207 kb on - strand, within DVU0442 at 500.348 kb on + strand, within DVU0442 at 500.349 kb on + strand, within DVU0442 at 500.356 kb on - strand, within DVU0442 at 500.372 kb on - strand, within DVU0442 at 500.382 kb on - strand, within DVU0442 at 500.428 kb on + strand, within DVU0442 at 500.428 kb on + strand, within DVU0442 at 500.431 kb on - strand, within DVU0442 at 500.534 kb on + strand, within DVU0442 at 500.615 kb on - strand, within DVU0442 at 500.633 kb on - strand, within DVU0442 at 500.732 kb on + strand, within DVU0442 at 500.816 kb on - strand, within DVU0442 at 500.818 kb on + strand, within DVU0442 at 500.826 kb on - strand, within DVU0442 at 500.947 kb on + strand at 501.075 kb on - strand at 501.090 kb on + strand at 501.210 kb on - strand at 501.218 kb on + strand at 501.242 kb on - strand at 501.258 kb on - strand at 501.386 kb on + strand, within DVU0443 at 501.394 kb on - strand, within DVU0443 at 501.394 kb on - strand, within DVU0443 at 501.454 kb on - strand, within DVU0443 at 501.474 kb on - strand, within DVU0443 at 501.476 kb on - strand, within DVU0443 at 501.598 kb on + strand, within DVU0443 at 501.774 kb on + strand, within DVU0443 at 501.797 kb on - strand, within DVU0443 at 501.855 kb on + strand, within DVU0443 at 501.863 kb on - strand at 501.925 kb on + strand at 501.945 kb on + strand at 501.953 kb on + strand at 501.953 kb on + strand at 501.960 kb on - strand at 501.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Threonine (N) remove 498,812 + ade DVU0441 0.69 -0.6 498,839 + ade DVU0441 0.71 -0.6 498,866 - ade DVU0441 0.73 -1.3 498,866 - ade DVU0441 0.73 -1.1 498,906 + ade DVU0441 0.75 -0.6 498,928 + ade DVU0441 0.76 -0.5 498,936 - ade DVU0441 0.77 -0.5 499,229 - +2.3 499,305 - -0.3 499,336 + -2.3 499,344 - -1.1 499,344 - +2.7 499,394 + -0.6 499,402 - +2.2 499,402 - +0.2 499,402 - -0.2 499,402 - -0.9 499,489 + +0.5 499,497 - -1.4 499,497 - +0.2 499,497 - +0.5 499,509 + +2.2 499,534 + +0.8 499,537 - -1.4 499,547 - -0.3 499,565 - +2.6 499,580 + +1.6 499,730 + -1.0 499,738 - -2.7 499,740 - -0.1 499,767 + +0.8 499,880 - -0.1 499,880 - -0.2 499,895 + +0.4 499,895 + +1.3 499,971 + DVU0442 0.15 -0.7 499,972 - DVU0442 0.15 +0.1 499,985 + DVU0442 0.17 +1.1 500,018 + DVU0442 0.19 +2.5 500,026 - DVU0442 0.20 +0.6 500,026 - DVU0442 0.20 -0.1 500,026 - DVU0442 0.20 -1.1 500,084 - DVU0442 0.25 +0.0 500,106 - DVU0442 0.27 +2.2 500,133 + DVU0442 0.29 -0.6 500,183 + DVU0442 0.33 -2.9 500,191 - DVU0442 0.34 +2.2 500,199 + DVU0442 0.34 -0.2 500,207 - DVU0442 0.35 +0.2 500,348 + DVU0442 0.47 -0.4 500,349 + DVU0442 0.47 -0.9 500,356 - DVU0442 0.48 +1.3 500,372 - DVU0442 0.49 -0.5 500,382 - DVU0442 0.50 -0.1 500,428 + DVU0442 0.54 -1.1 500,428 + DVU0442 0.54 -0.9 500,431 - DVU0442 0.54 -1.1 500,534 + DVU0442 0.62 +2.6 500,615 - DVU0442 0.69 -0.2 500,633 - DVU0442 0.71 -1.0 500,732 + DVU0442 0.79 -1.9 500,816 - DVU0442 0.86 -0.0 500,818 + DVU0442 0.86 -1.3 500,826 - DVU0442 0.87 +0.2 500,947 + -0.7 501,075 - -3.4 501,090 + +0.0 501,210 - -0.6 501,218 + +1.8 501,242 - +0.2 501,258 - -1.1 501,386 + DVU0443 0.26 -1.0 501,394 - DVU0443 0.27 +0.5 501,394 - DVU0443 0.27 -0.2 501,454 - DVU0443 0.35 +0.1 501,474 - DVU0443 0.38 +2.4 501,476 - DVU0443 0.38 -1.4 501,598 + DVU0443 0.55 -0.6 501,774 + DVU0443 0.79 -0.2 501,797 - DVU0443 0.82 +0.2 501,855 + DVU0443 0.90 +4.9 501,863 - +1.1 501,925 + -1.3 501,945 + +0.5 501,953 + -1.3 501,953 + +1.9 501,960 - -0.4 501,966 - +0.3
Or see this region's nucleotide sequence