Experiment: MoLS4 with L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvH and DVU0362 are separated by 50 nucleotides DVU0362 and pabB overlap by 4 nucleotides pabB and pabA are separated by 538 nucleotides
DVU0361: ilvH - acetolactate synthase III, small subunit, putative (TIGR), at 411,087 to 411,410
ilvH
DVU0362: DVU0362 - conserved hypothetical protein (TIGR), at 411,461 to 412,240
DVU0362
DVU0363: pabB - para-aminobenzoate synthase, component I (TIGR), at 412,237 to 413,241
pabB
DVU0364: pabA - para-aminobenzoate/anthranilate synthase glutamine amidotransferase (TIGR), at 413,780 to 414,334
pabA
Position (kb) 412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 411.373 kb on + strand, within ilvH at 411.389 kb on - strand at 411.409 kb on + strand at 411.532 kb on - strand at 411.565 kb on - strand, within DVU0362 at 411.626 kb on - strand, within DVU0362 at 411.626 kb on - strand, within DVU0362 at 411.631 kb on - strand, within DVU0362 at 411.642 kb on + strand, within DVU0362 at 411.649 kb on - strand, within DVU0362 at 411.683 kb on - strand, within DVU0362 at 411.708 kb on - strand, within DVU0362 at 411.727 kb on - strand, within DVU0362 at 411.915 kb on + strand, within DVU0362 at 411.938 kb on + strand, within DVU0362 at 411.996 kb on - strand, within DVU0362 at 412.062 kb on - strand, within DVU0362 at 412.093 kb on - strand, within DVU0362 at 412.134 kb on + strand, within DVU0362 at 412.146 kb on + strand, within DVU0362 at 412.344 kb on + strand, within pabB at 412.360 kb on + strand, within pabB at 412.360 kb on + strand, within pabB at 412.368 kb on - strand, within pabB at 412.368 kb on - strand, within pabB at 412.485 kb on + strand, within pabB at 412.493 kb on - strand, within pabB at 412.503 kb on - strand, within pabB at 412.528 kb on - strand, within pabB at 412.590 kb on - strand, within pabB at 412.602 kb on + strand, within pabB at 412.664 kb on + strand, within pabB at 412.738 kb on + strand, within pabB at 412.738 kb on - strand, within pabB at 412.770 kb on + strand, within pabB at 412.778 kb on - strand, within pabB at 412.778 kb on - strand, within pabB at 412.787 kb on + strand, within pabB at 412.828 kb on + strand, within pabB at 412.834 kb on - strand, within pabB at 412.901 kb on - strand, within pabB at 412.980 kb on + strand, within pabB at 412.983 kb on - strand, within pabB at 412.996 kb on - strand, within pabB at 412.996 kb on - strand, within pabB at 413.002 kb on + strand, within pabB at 413.019 kb on + strand, within pabB at 413.027 kb on - strand, within pabB at 413.094 kb on - strand, within pabB at 413.097 kb on + strand, within pabB at 413.128 kb on + strand, within pabB at 413.205 kb on + strand at 413.264 kb on + strand at 413.268 kb on - strand at 413.306 kb on - strand at 413.430 kb on - strand at 413.523 kb on - strand at 413.531 kb on - strand at 413.549 kb on - strand at 413.570 kb on + strand at 413.577 kb on + strand at 413.578 kb on - strand at 413.578 kb on - strand at 413.587 kb on - strand at 413.641 kb on + strand at 413.680 kb on + strand at 413.709 kb on - strand at 413.719 kb on - strand at 413.755 kb on + strand at 413.796 kb on - strand at 413.811 kb on - strand at 413.858 kb on - strand, within pabA at 413.935 kb on + strand, within pabA at 413.964 kb on + strand, within pabA at 413.977 kb on - strand, within pabA at 413.990 kb on - strand, within pabA at 414.007 kb on - strand, within pabA at 414.012 kb on + strand, within pabA at 414.018 kb on + strand, within pabA at 414.020 kb on - strand, within pabA at 414.103 kb on + strand, within pabA at 414.149 kb on - strand, within pabA at 414.186 kb on - strand, within pabA at 414.224 kb on + strand, within pabA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Threonine (N) remove 411,373 + ilvH DVU0361 0.88 -2.2 411,389 - +2.2 411,409 + -0.6 411,532 - -0.1 411,565 - DVU0362 0.13 +0.8 411,626 - DVU0362 0.21 -0.9 411,626 - DVU0362 0.21 -0.3 411,631 - DVU0362 0.22 -1.4 411,642 + DVU0362 0.23 +1.8 411,649 - DVU0362 0.24 -1.0 411,683 - DVU0362 0.28 -0.6 411,708 - DVU0362 0.32 -1.0 411,727 - DVU0362 0.34 -1.7 411,915 + DVU0362 0.58 -0.1 411,938 + DVU0362 0.61 -2.0 411,996 - DVU0362 0.69 -0.2 412,062 - DVU0362 0.77 -1.9 412,093 - DVU0362 0.81 -1.1 412,134 + DVU0362 0.86 +3.8 412,146 + DVU0362 0.88 +0.0 412,344 + pabB DVU0363 0.11 -0.6 412,360 + pabB DVU0363 0.12 -1.4 412,360 + pabB DVU0363 0.12 -0.1 412,368 - pabB DVU0363 0.13 +0.4 412,368 - pabB DVU0363 0.13 +0.7 412,485 + pabB DVU0363 0.25 +2.8 412,493 - pabB DVU0363 0.25 +1.0 412,503 - pabB DVU0363 0.26 -0.2 412,528 - pabB DVU0363 0.29 -0.2 412,590 - pabB DVU0363 0.35 -2.3 412,602 + pabB DVU0363 0.36 -0.5 412,664 + pabB DVU0363 0.42 -1.0 412,738 + pabB DVU0363 0.50 +1.0 412,738 - pabB DVU0363 0.50 +0.3 412,770 + pabB DVU0363 0.53 +0.4 412,778 - pabB DVU0363 0.54 +0.6 412,778 - pabB DVU0363 0.54 -1.2 412,787 + pabB DVU0363 0.55 -3.0 412,828 + pabB DVU0363 0.59 -0.3 412,834 - pabB DVU0363 0.59 -0.5 412,901 - pabB DVU0363 0.66 -0.5 412,980 + pabB DVU0363 0.74 -1.4 412,983 - pabB DVU0363 0.74 -0.5 412,996 - pabB DVU0363 0.76 -0.6 412,996 - pabB DVU0363 0.76 -0.6 413,002 + pabB DVU0363 0.76 +0.2 413,019 + pabB DVU0363 0.78 -1.0 413,027 - pabB DVU0363 0.79 -0.8 413,094 - pabB DVU0363 0.85 +1.3 413,097 + pabB DVU0363 0.86 -0.1 413,128 + pabB DVU0363 0.89 -0.4 413,205 + -0.6 413,264 + -0.6 413,268 - -1.4 413,306 - +1.0 413,430 - -0.3 413,523 - -1.4 413,531 - +0.8 413,549 - -1.3 413,570 + +0.8 413,577 + -2.3 413,578 - +0.2 413,578 - +3.5 413,587 - +0.4 413,641 + -1.6 413,680 + -1.1 413,709 - +0.4 413,719 - +1.0 413,755 + -0.3 413,796 - -1.8 413,811 - -0.9 413,858 - pabA DVU0364 0.14 -1.6 413,935 + pabA DVU0364 0.28 +3.3 413,964 + pabA DVU0364 0.33 +1.8 413,977 - pabA DVU0364 0.35 +0.0 413,990 - pabA DVU0364 0.38 +0.2 414,007 - pabA DVU0364 0.41 -0.9 414,012 + pabA DVU0364 0.42 -0.9 414,018 + pabA DVU0364 0.43 +4.0 414,020 - pabA DVU0364 0.43 -0.5 414,103 + pabA DVU0364 0.58 -2.7 414,149 - pabA DVU0364 0.66 -1.0 414,186 - pabA DVU0364 0.73 -1.0 414,224 + pabA DVU0364 0.80 -0.7
Or see this region's nucleotide sequence