Experiment: MoLS4 with L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serA and DVU0340 are separated by 142 nucleotides DVU0340 and kdsB are separated by 9 nucleotides kdsB and DVU0342 are separated by 312 nucleotides
DVU0339: serA - D-isomer specific 2-hydroxyacid dehydrogenase family protein (TIGR), at 384,768 to 385,673
serA
DVU0340: DVU0340 - acetyltransferase, CysE/LacA/LpxA/NodL family (TIGR), at 385,816 to 386,409
DVU0340
DVU0341: kdsB - 3-deoxy-D-manno-octulosonate cytidylyltransferase (TIGR), at 386,419 to 387,189
kdsB
DVU0342: DVU0342 - NAD-dependent epimerase/dehydratase family protein (TIGR), at 387,502 to 388,416
DVU0342
Position (kb) 386
387
388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 385.431 kb on + strand, within serA at 385.431 kb on + strand, within serA at 385.431 kb on + strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.530 kb on + strand, within serA at 385.617 kb on + strand at 385.625 kb on - strand at 385.625 kb on - strand at 385.686 kb on + strand at 385.760 kb on + strand at 385.761 kb on + strand at 385.796 kb on - strand at 385.801 kb on - strand at 385.803 kb on + strand at 385.821 kb on + strand at 385.879 kb on - strand, within DVU0340 at 385.879 kb on - strand, within DVU0340 at 385.907 kb on + strand, within DVU0340 at 385.950 kb on - strand, within DVU0340 at 386.159 kb on + strand, within DVU0340 at 386.167 kb on + strand, within DVU0340 at 386.167 kb on - strand, within DVU0340 at 386.191 kb on - strand, within DVU0340 at 386.230 kb on + strand, within DVU0340 at 386.305 kb on - strand, within DVU0340 at 386.333 kb on + strand, within DVU0340 at 386.356 kb on - strand at 386.372 kb on + strand at 386.374 kb on + strand at 386.382 kb on - strand at 387.217 kb on + strand at 387.217 kb on + strand at 387.253 kb on - strand at 387.311 kb on - strand at 387.334 kb on - strand at 387.426 kb on - strand at 387.520 kb on - strand at 387.528 kb on + strand at 387.551 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Threonine (N) remove 385,431 + serA DVU0339 0.73 +0.2 385,431 + serA DVU0339 0.73 -1.4 385,431 + serA DVU0339 0.73 +0.5 385,439 - serA DVU0339 0.74 +0.0 385,439 - serA DVU0339 0.74 -1.0 385,439 - serA DVU0339 0.74 -0.1 385,439 - serA DVU0339 0.74 +0.3 385,439 - serA DVU0339 0.74 -0.4 385,530 + serA DVU0339 0.84 +1.5 385,617 + +0.2 385,625 - +1.4 385,625 - +0.1 385,686 + -0.5 385,760 + -1.1 385,761 + -0.2 385,796 - -1.0 385,801 - -0.5 385,803 + -3.7 385,821 + +0.8 385,879 - DVU0340 0.11 -0.8 385,879 - DVU0340 0.11 -0.1 385,907 + DVU0340 0.15 +2.3 385,950 - DVU0340 0.23 -0.6 386,159 + DVU0340 0.58 +2.5 386,167 + DVU0340 0.59 +3.2 386,167 - DVU0340 0.59 +2.1 386,191 - DVU0340 0.63 +0.9 386,230 + DVU0340 0.70 -0.5 386,305 - DVU0340 0.82 +1.4 386,333 + DVU0340 0.87 -1.4 386,356 - -0.7 386,372 + +1.8 386,374 + -1.9 386,382 - -0.7 387,217 + +1.9 387,217 + +0.3 387,253 - -1.1 387,311 - -0.7 387,334 - -0.6 387,426 - -0.3 387,520 - +0.2 387,528 + -1.0 387,551 - -0.7
Or see this region's nucleotide sequence