Experiment: H2 N-free with 10mM L-Asparagine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02720 and MMP_RS02725 are separated by 10 nucleotides MMP_RS02725 and MMP_RS02730 are separated by 123 nucleotides MMP_RS02730 and MMP_RS02735 are separated by 27 nucleotides MMP_RS02735 and MMP_RS02740 are separated by 80 nucleotides
MMP_RS02720: MMP_RS02720 - molybdate ABC transporter substrate-binding protein, at 520,134 to 520,910
_RS02720
MMP_RS02725: MMP_RS02725 - molybdate ABC transporter permease subunit, at 520,921 to 521,586
_RS02725
MMP_RS02730: MMP_RS02730 - ModD protein, at 521,710 to 522,561
_RS02730
MMP_RS02735: MMP_RS02735 - MBL fold metallo-hydrolase, at 522,589 to 523,119
_RS02735
MMP_RS02740: MMP_RS02740 - hypothetical protein, at 523,200 to 523,469
_RS02740
Position (kb) 521
522
523 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 520.711 kb on - strand, within MMP_RS02720 at 520.852 kb on + strand at 520.950 kb on - strand at 520.971 kb on + strand at 521.004 kb on - strand, within MMP_RS02725 at 521.004 kb on - strand, within MMP_RS02725 at 521.063 kb on - strand, within MMP_RS02725 at 521.064 kb on + strand, within MMP_RS02725 at 521.096 kb on - strand, within MMP_RS02725 at 521.105 kb on - strand, within MMP_RS02725 at 521.291 kb on + strand, within MMP_RS02725 at 521.369 kb on - strand, within MMP_RS02725 at 521.377 kb on + strand, within MMP_RS02725 at 521.463 kb on - strand, within MMP_RS02725 at 521.511 kb on + strand, within MMP_RS02725 at 521.524 kb on + strand at 521.546 kb on - strand at 521.546 kb on - strand at 521.547 kb on + strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.549 kb on + strand at 521.549 kb on + strand at 521.549 kb on + strand at 521.553 kb on + strand at 521.572 kb on + strand at 521.621 kb on + strand at 521.621 kb on + strand at 521.621 kb on + strand at 521.623 kb on + strand at 521.635 kb on - strand at 521.635 kb on - strand at 521.636 kb on + strand at 521.636 kb on + strand at 521.636 kb on + strand at 521.672 kb on - strand at 521.675 kb on - strand at 521.676 kb on + strand at 521.680 kb on + strand at 521.709 kb on - strand at 521.710 kb on + strand at 521.710 kb on + strand at 521.719 kb on - strand at 521.719 kb on - strand at 521.825 kb on + strand, within MMP_RS02730 at 521.876 kb on - strand, within MMP_RS02730 at 521.876 kb on - strand, within MMP_RS02730 at 521.877 kb on + strand, within MMP_RS02730 at 522.134 kb on - strand, within MMP_RS02730 at 522.143 kb on - strand, within MMP_RS02730 at 522.189 kb on - strand, within MMP_RS02730 at 522.189 kb on - strand, within MMP_RS02730 at 522.189 kb on - strand, within MMP_RS02730 at 522.190 kb on + strand, within MMP_RS02730 at 522.190 kb on + strand, within MMP_RS02730 at 522.197 kb on - strand, within MMP_RS02730 at 522.461 kb on - strand, within MMP_RS02730 at 522.469 kb on - strand, within MMP_RS02730 at 522.542 kb on - strand at 522.563 kb on - strand at 522.583 kb on - strand at 522.586 kb on + strand at 522.719 kb on + strand, within MMP_RS02735 at 522.759 kb on + strand, within MMP_RS02735 at 522.759 kb on + strand, within MMP_RS02735 at 522.811 kb on + strand, within MMP_RS02735 at 522.833 kb on + strand, within MMP_RS02735 at 522.922 kb on - strand, within MMP_RS02735 at 522.931 kb on - strand, within MMP_RS02735 at 523.013 kb on + strand, within MMP_RS02735 at 523.174 kb on + strand at 523.186 kb on - strand at 523.191 kb on - strand at 523.299 kb on - strand, within MMP_RS02740 at 523.368 kb on - strand, within MMP_RS02740 at 523.397 kb on - strand, within MMP_RS02740 at 523.414 kb on + strand, within MMP_RS02740 at 523.515 kb on - strand at 523.515 kb on - strand at 523.517 kb on - strand at 523.520 kb on - strand at 523.521 kb on + strand at 523.523 kb on + strand at 523.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Asparagine remove 520,711 - MMP_RS02720 0.74 -0.4 520,852 + -0.3 520,950 - +0.7 520,971 + +0.4 521,004 - MMP_RS02725 0.12 +0.4 521,004 - MMP_RS02725 0.12 +0.7 521,063 - MMP_RS02725 0.21 -1.0 521,064 + MMP_RS02725 0.21 -0.5 521,096 - MMP_RS02725 0.26 -0.6 521,105 - MMP_RS02725 0.28 +0.2 521,291 + MMP_RS02725 0.56 -0.7 521,369 - MMP_RS02725 0.67 -0.2 521,377 + MMP_RS02725 0.68 +0.2 521,463 - MMP_RS02725 0.81 -1.0 521,511 + MMP_RS02725 0.89 -0.9 521,524 + +0.3 521,546 - -0.7 521,546 - +0.3 521,547 + +0.7 521,548 - -0.6 521,548 - +0.8 521,548 - +0.1 521,548 - +0.1 521,548 - -0.1 521,549 + +0.3 521,549 + -0.1 521,549 + -0.0 521,553 + +0.5 521,572 + +0.9 521,621 + -1.4 521,621 + -0.4 521,621 + -0.9 521,623 + -1.4 521,635 - -3.7 521,635 - -3.1 521,636 + -1.0 521,636 + -2.1 521,636 + +0.1 521,672 - -3.1 521,675 - -2.2 521,676 + -1.5 521,680 + -0.4 521,709 - -3.3 521,710 + -3.0 521,710 + -2.7 521,719 - -4.0 521,719 - -4.3 521,825 + MMP_RS02730 0.13 -3.1 521,876 - MMP_RS02730 0.19 -3.5 521,876 - MMP_RS02730 0.19 -3.3 521,877 + MMP_RS02730 0.20 -2.9 522,134 - MMP_RS02730 0.50 -3.6 522,143 - MMP_RS02730 0.51 -3.2 522,189 - MMP_RS02730 0.56 -4.4 522,189 - MMP_RS02730 0.56 -1.8 522,189 - MMP_RS02730 0.56 -3.8 522,190 + MMP_RS02730 0.56 -3.5 522,190 + MMP_RS02730 0.56 -2.5 522,197 - MMP_RS02730 0.57 -3.0 522,461 - MMP_RS02730 0.88 -3.7 522,469 - MMP_RS02730 0.89 -3.3 522,542 - -2.7 522,563 - -0.2 522,583 - -0.4 522,586 + +1.1 522,719 + MMP_RS02735 0.24 +0.2 522,759 + MMP_RS02735 0.32 +0.9 522,759 + MMP_RS02735 0.32 -0.8 522,811 + MMP_RS02735 0.42 +1.1 522,833 + MMP_RS02735 0.46 -0.1 522,922 - MMP_RS02735 0.63 -0.8 522,931 - MMP_RS02735 0.64 +1.0 523,013 + MMP_RS02735 0.80 +0.7 523,174 + -0.1 523,186 - +0.2 523,191 - +0.8 523,299 - MMP_RS02740 0.37 +0.8 523,368 - MMP_RS02740 0.62 +0.4 523,397 - MMP_RS02740 0.73 -0.4 523,414 + MMP_RS02740 0.79 -0.1 523,515 - +0.2 523,515 - +0.3 523,517 - +0.6 523,520 - +0.0 523,521 + +0.0 523,523 + -0.4 523,525 - +0.9
Or see this region's nucleotide sequence