Experiment: H2 N-free with 10mM L-Asparagine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02225 and MMP_RS02230 are separated by 142 nucleotides MMP_RS02230 and MMP_RS08940 are separated by 33 nucleotides MMP_RS08940 and MMP_RS02235 are separated by 195 nucleotides
MMP_RS02225: MMP_RS02225 - carbohydrate kinase family protein, at 419,415 to 420,323
_RS02225
MMP_RS02230: MMP_RS02230 - sodium-dependent transporter, at 420,466 to 421,977
_RS02230
MMP_RS08940: MMP_RS08940 - MetS family NSS transporter small subunit, at 422,011 to 422,091
_RS08940
MMP_RS02235: MMP_RS02235 - CBS domain-containing protein, at 422,287 to 423,252
_RS02235
Position (kb) 420
421
422 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 419.594 kb on + strand, within MMP_RS02225 at 419.640 kb on - strand, within MMP_RS02225 at 419.812 kb on - strand, within MMP_RS02225 at 419.813 kb on + strand, within MMP_RS02225 at 419.914 kb on - strand, within MMP_RS02225 at 419.967 kb on + strand, within MMP_RS02225 at 420.047 kb on + strand, within MMP_RS02225 at 420.080 kb on + strand, within MMP_RS02225 at 420.081 kb on - strand, within MMP_RS02225 at 420.352 kb on - strand at 420.408 kb on + strand at 420.414 kb on + strand at 420.414 kb on + strand at 420.750 kb on + strand, within MMP_RS02230 at 420.792 kb on + strand, within MMP_RS02230 at 420.793 kb on - strand, within MMP_RS02230 at 420.855 kb on - strand, within MMP_RS02230 at 420.898 kb on + strand, within MMP_RS02230 at 420.930 kb on + strand, within MMP_RS02230 at 420.943 kb on - strand, within MMP_RS02230 at 420.943 kb on - strand, within MMP_RS02230 at 420.943 kb on - strand, within MMP_RS02230 at 421.078 kb on - strand, within MMP_RS02230 at 421.107 kb on + strand, within MMP_RS02230 at 421.116 kb on - strand, within MMP_RS02230 at 421.326 kb on - strand, within MMP_RS02230 at 421.359 kb on + strand, within MMP_RS02230 at 421.409 kb on - strand, within MMP_RS02230 at 421.410 kb on + strand, within MMP_RS02230 at 421.410 kb on + strand, within MMP_RS02230 at 421.422 kb on + strand, within MMP_RS02230 at 421.615 kb on - strand, within MMP_RS02230 at 421.784 kb on + strand, within MMP_RS02230 at 421.983 kb on - strand at 422.009 kb on - strand at 422.010 kb on + strand at 422.036 kb on - strand, within MMP_RS08940 at 422.089 kb on - strand at 422.090 kb on + strand at 422.092 kb on - strand at 422.093 kb on + strand at 422.160 kb on - strand at 422.163 kb on + strand at 422.181 kb on - strand at 422.227 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.232 kb on - strand at 422.233 kb on + strand at 422.233 kb on + strand at 422.234 kb on - strand at 422.235 kb on + strand at 422.241 kb on - strand at 422.291 kb on - strand at 422.292 kb on + strand at 422.321 kb on - strand at 422.323 kb on - strand at 422.323 kb on - strand at 422.324 kb on + strand at 422.324 kb on + strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.352 kb on - strand at 422.356 kb on - strand at 422.356 kb on - strand at 422.357 kb on + strand at 422.357 kb on + strand at 422.360 kb on - strand at 422.368 kb on - strand at 422.368 kb on - strand at 422.372 kb on - strand at 422.376 kb on - strand at 422.376 kb on - strand at 422.456 kb on - strand, within MMP_RS02235 at 422.456 kb on - strand, within MMP_RS02235 at 422.457 kb on + strand, within MMP_RS02235 at 422.471 kb on - strand, within MMP_RS02235 at 422.472 kb on + strand, within MMP_RS02235 at 422.518 kb on + strand, within MMP_RS02235 at 422.554 kb on + strand, within MMP_RS02235 at 422.623 kb on + strand, within MMP_RS02235 at 422.651 kb on - strand, within MMP_RS02235 at 422.794 kb on + strand, within MMP_RS02235 at 422.829 kb on - strand, within MMP_RS02235 at 422.863 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Asparagine remove 419,594 + MMP_RS02225 0.20 -0.4 419,640 - MMP_RS02225 0.25 -0.3 419,812 - MMP_RS02225 0.44 +0.0 419,813 + MMP_RS02225 0.44 -1.9 419,914 - MMP_RS02225 0.55 -1.3 419,967 + MMP_RS02225 0.61 +0.3 420,047 + MMP_RS02225 0.70 +0.3 420,080 + MMP_RS02225 0.73 -0.0 420,081 - MMP_RS02225 0.73 -0.5 420,352 - -0.2 420,408 + -1.2 420,414 + -1.3 420,414 + -2.1 420,750 + MMP_RS02230 0.19 -1.7 420,792 + MMP_RS02230 0.22 -0.8 420,793 - MMP_RS02230 0.22 -1.8 420,855 - MMP_RS02230 0.26 -0.4 420,898 + MMP_RS02230 0.29 -1.2 420,930 + MMP_RS02230 0.31 -0.0 420,943 - MMP_RS02230 0.32 -1.0 420,943 - MMP_RS02230 0.32 -1.1 420,943 - MMP_RS02230 0.32 -1.2 421,078 - MMP_RS02230 0.40 -1.5 421,107 + MMP_RS02230 0.42 -0.6 421,116 - MMP_RS02230 0.43 -0.7 421,326 - MMP_RS02230 0.57 -1.2 421,359 + MMP_RS02230 0.59 -1.1 421,409 - MMP_RS02230 0.62 -1.4 421,410 + MMP_RS02230 0.62 -3.3 421,410 + MMP_RS02230 0.62 -1.6 421,422 + MMP_RS02230 0.63 -1.5 421,615 - MMP_RS02230 0.76 -2.6 421,784 + MMP_RS02230 0.87 -1.9 421,983 - +0.9 422,009 - +0.9 422,010 + +0.9 422,036 - MMP_RS08940 0.31 -0.5 422,089 - +0.2 422,090 + +0.5 422,092 - -0.3 422,093 + -0.2 422,160 - -0.3 422,163 + +0.8 422,181 - +0.0 422,227 - -0.7 422,232 - -1.5 422,232 - -0.6 422,232 - -0.2 422,232 - -0.6 422,232 - -1.6 422,233 + -0.1 422,233 + +0.0 422,234 - -0.7 422,235 + -1.3 422,241 - -1.7 422,291 - -0.3 422,292 + -0.7 422,321 - -0.9 422,323 - -0.6 422,323 - -1.3 422,324 + -0.7 422,324 + -1.1 422,325 - -1.1 422,325 - -1.2 422,325 - -1.2 422,326 + -1.7 422,326 + -1.3 422,326 + -0.2 422,352 - -1.4 422,356 - -1.2 422,356 - -1.0 422,357 + -1.4 422,357 + -1.1 422,360 - -1.4 422,368 - +0.1 422,368 - -0.9 422,372 - +0.0 422,376 - -0.8 422,376 - -1.6 422,456 - MMP_RS02235 0.17 -0.6 422,456 - MMP_RS02235 0.17 -1.0 422,457 + MMP_RS02235 0.18 -1.9 422,471 - MMP_RS02235 0.19 -0.4 422,472 + MMP_RS02235 0.19 -1.6 422,518 + MMP_RS02235 0.24 -2.1 422,554 + MMP_RS02235 0.28 -1.2 422,623 + MMP_RS02235 0.35 -0.7 422,651 - MMP_RS02235 0.38 -0.5 422,794 + MMP_RS02235 0.52 -0.4 422,829 - MMP_RS02235 0.56 -1.0 422,863 - MMP_RS02235 0.60 -1.1 422,938 - MMP_RS02235 0.67 -0.5 422,938 - MMP_RS02235 0.67 -0.5
Or see this region's nucleotide sequence