Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23000 and BWI76_RS23005 are separated by 74 nucleotides BWI76_RS23005 and BWI76_RS23010 overlap by 7 nucleotides
BWI76_RS23000: BWI76_RS23000 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,798,846 to 4,799,901
_RS23000
BWI76_RS23005: BWI76_RS23005 - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 4,799,976 to 4,800,521
_RS23005
BWI76_RS23010: BWI76_RS23010 - cobyric acid synthase CobQ, at 4,800,515 to 4,802,038
_RS23010
Position (kb)
4799
4800
4801 Strain fitness (log2 ratio)
-2
-1
0
1 at 4799.001 kb on + strand, within BWI76_RS23000 at 4799.051 kb on - strand, within BWI76_RS23000 at 4799.933 kb on - strand at 4800.011 kb on + strand at 4800.013 kb on - strand at 4800.126 kb on - strand, within BWI76_RS23005 at 4800.368 kb on - strand, within BWI76_RS23005 at 4800.650 kb on + strand at 4800.697 kb on + strand, within BWI76_RS23010 at 4800.891 kb on + strand, within BWI76_RS23010 at 4800.920 kb on - strand, within BWI76_RS23010 at 4800.938 kb on - strand, within BWI76_RS23010 at 4801.437 kb on - strand, within BWI76_RS23010 at 4801.464 kb on + strand, within BWI76_RS23010 at 4801.464 kb on + strand, within BWI76_RS23010 at 4801.472 kb on - strand, within BWI76_RS23010 at 4801.472 kb on - strand, within BWI76_RS23010 at 4801.472 kb on - strand, within BWI76_RS23010
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,799,001 + BWI76_RS23000 0.15 -0.1 4,799,051 - BWI76_RS23000 0.19 +0.1 4,799,933 - -0.4 4,800,011 + -0.4 4,800,013 - -0.1 4,800,126 - BWI76_RS23005 0.27 +0.4 4,800,368 - BWI76_RS23005 0.72 +0.6 4,800,650 + +0.5 4,800,697 + BWI76_RS23010 0.12 +0.1 4,800,891 + BWI76_RS23010 0.25 -1.9 4,800,920 - BWI76_RS23010 0.27 -2.0 4,800,938 - BWI76_RS23010 0.28 -0.5 4,801,437 - BWI76_RS23010 0.60 +0.1 4,801,464 + BWI76_RS23010 0.62 +0.3 4,801,464 + BWI76_RS23010 0.62 -0.3 4,801,472 - BWI76_RS23010 0.63 +0.1 4,801,472 - BWI76_RS23010 0.63 -0.1 4,801,472 - BWI76_RS23010 0.63 +0.6
Or see this region's nucleotide sequence