Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20240 and BWI76_RS20245 are separated by 46 nucleotides BWI76_RS20245 and BWI76_RS20250 overlap by 8 nucleotides BWI76_RS20250 and BWI76_RS20255 overlap by 4 nucleotides
BWI76_RS20240: BWI76_RS20240 - phosphate acetyltransferase, at 4,241,656 to 4,243,800
_RS20240
BWI76_RS20245: BWI76_RS20245 - transketolase subunit B, at 4,243,847 to 4,244,800
_RS20245
BWI76_RS20250: BWI76_RS20250 - hypothetical protein, at 4,244,793 to 4,245,623
_RS20250
BWI76_RS20255: BWI76_RS20255 - PTS ascorbate transporter subunit IIC, at 4,245,620 to 4,247,011
_RS20255
Position (kb) 4243
4244
4245 Strain fitness (log2 ratio) -1
0
1 at 4242.998 kb on + strand, within BWI76_RS20240 at 4243.710 kb on + strand at 4243.750 kb on + strand at 4243.928 kb on - strand at 4243.942 kb on + strand at 4243.976 kb on + strand, within BWI76_RS20245 at 4244.137 kb on - strand, within BWI76_RS20245 at 4244.455 kb on + strand, within BWI76_RS20245 at 4244.507 kb on - strand, within BWI76_RS20245 at 4244.668 kb on + strand, within BWI76_RS20245 at 4244.742 kb on + strand at 4244.791 kb on + strand at 4244.901 kb on + strand, within BWI76_RS20250 at 4245.094 kb on - strand, within BWI76_RS20250 at 4245.094 kb on - strand, within BWI76_RS20250 at 4245.290 kb on + strand, within BWI76_RS20250 at 4245.355 kb on - strand, within BWI76_RS20250 at 4245.434 kb on + strand, within BWI76_RS20250 at 4245.462 kb on - strand, within BWI76_RS20250 at 4245.633 kb on + strand at 4245.638 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,242,998 + BWI76_RS20240 0.63 +0.5 4,243,710 + -0.8 4,243,750 + +1.6 4,243,928 - +0.4 4,243,942 + -1.0 4,243,976 + BWI76_RS20245 0.14 +0.5 4,244,137 - BWI76_RS20245 0.30 +0.6 4,244,455 + BWI76_RS20245 0.64 +1.0 4,244,507 - BWI76_RS20245 0.69 -0.1 4,244,668 + BWI76_RS20245 0.86 +1.0 4,244,742 + -0.1 4,244,791 + +0.2 4,244,901 + BWI76_RS20250 0.13 -0.3 4,245,094 - BWI76_RS20250 0.36 +0.1 4,245,094 - BWI76_RS20250 0.36 +0.2 4,245,290 + BWI76_RS20250 0.60 -1.3 4,245,355 - BWI76_RS20250 0.68 +0.7 4,245,434 + BWI76_RS20250 0.77 -0.5 4,245,462 - BWI76_RS20250 0.81 -1.0 4,245,633 + +0.6 4,245,638 + -1.2
Or see this region's nucleotide sequence