Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20205 and BWI76_RS20210 are separated by 87 nucleotides BWI76_RS20210 and BWI76_RS20215 are separated by 53 nucleotides
BWI76_RS20205: BWI76_RS20205 - aminotransferase AlaT, at 4,234,466 to 4,235,683
_RS20205
BWI76_RS20210: BWI76_RS20210 - 5'-deoxynucleotidase, at 4,235,771 to 4,236,370
_RS20210
BWI76_RS20215: BWI76_RS20215 - transcriptional regulator, at 4,236,424 to 4,238,256
_RS20215
Position (kb)
4235
4236
4237 Strain fitness (log2 ratio)
-2
-1
0
1 at 4234.908 kb on + strand, within BWI76_RS20205 at 4235.130 kb on - strand, within BWI76_RS20205 at 4235.207 kb on + strand, within BWI76_RS20205 at 4235.291 kb on - strand, within BWI76_RS20205 at 4235.390 kb on - strand, within BWI76_RS20205 at 4235.442 kb on - strand, within BWI76_RS20205 at 4235.459 kb on + strand, within BWI76_RS20205 at 4235.459 kb on + strand, within BWI76_RS20205 at 4235.757 kb on + strand at 4235.757 kb on + strand at 4235.872 kb on - strand, within BWI76_RS20210 at 4235.935 kb on + strand, within BWI76_RS20210 at 4235.943 kb on - strand, within BWI76_RS20210 at 4235.943 kb on - strand, within BWI76_RS20210 at 4235.943 kb on - strand, within BWI76_RS20210 at 4236.053 kb on - strand, within BWI76_RS20210 at 4236.285 kb on + strand, within BWI76_RS20210 at 4236.290 kb on + strand, within BWI76_RS20210 at 4236.390 kb on + strand at 4236.458 kb on + strand at 4236.619 kb on - strand, within BWI76_RS20215 at 4236.684 kb on - strand, within BWI76_RS20215 at 4236.859 kb on + strand, within BWI76_RS20215 at 4237.010 kb on - strand, within BWI76_RS20215 at 4237.167 kb on - strand, within BWI76_RS20215 at 4237.265 kb on + strand, within BWI76_RS20215 at 4237.357 kb on - strand, within BWI76_RS20215
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,234,908 + BWI76_RS20205 0.36 -1.8 4,235,130 - BWI76_RS20205 0.55 +0.2 4,235,207 + BWI76_RS20205 0.61 -0.3 4,235,291 - BWI76_RS20205 0.68 -1.0 4,235,390 - BWI76_RS20205 0.76 +1.0 4,235,442 - BWI76_RS20205 0.80 +1.3 4,235,459 + BWI76_RS20205 0.82 +0.0 4,235,459 + BWI76_RS20205 0.82 +0.1 4,235,757 + +1.0 4,235,757 + +0.1 4,235,872 - BWI76_RS20210 0.17 +0.3 4,235,935 + BWI76_RS20210 0.27 -0.6 4,235,943 - BWI76_RS20210 0.29 -0.3 4,235,943 - BWI76_RS20210 0.29 +0.6 4,235,943 - BWI76_RS20210 0.29 +1.1 4,236,053 - BWI76_RS20210 0.47 +1.3 4,236,285 + BWI76_RS20210 0.86 +0.2 4,236,290 + BWI76_RS20210 0.86 +0.2 4,236,390 + -0.1 4,236,458 + -0.5 4,236,619 - BWI76_RS20215 0.11 -0.0 4,236,684 - BWI76_RS20215 0.14 +0.2 4,236,859 + BWI76_RS20215 0.24 +0.5 4,237,010 - BWI76_RS20215 0.32 -0.8 4,237,167 - BWI76_RS20215 0.41 -0.6 4,237,265 + BWI76_RS20215 0.46 +0.0 4,237,357 - BWI76_RS20215 0.51 +0.2
Or see this region's nucleotide sequence