Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19885 and BWI76_RS19890 are separated by 76 nucleotides BWI76_RS19890 and BWI76_RS19895 overlap by 1 nucleotides
BWI76_RS19885: BWI76_RS19885 - multidrug ABC transporter permease/ATP-binding protein, at 4,158,393 to 4,160,036
_RS19885
BWI76_RS19890: BWI76_RS19890 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 4,160,113 to 4,160,763
_RS19890
BWI76_RS19895: BWI76_RS19895 - bifunctional transcriptional regulator/O6-methylguanine-DNA methyltransferase, at 4,160,763 to 4,161,827
_RS19895
Position (kb)
4160
4161 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4159.167 kb on + strand, within BWI76_RS19885 at 4159.190 kb on - strand, within BWI76_RS19885 at 4159.909 kb on + strand at 4159.943 kb on + strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.951 kb on - strand at 4159.963 kb on - strand at 4160.078 kb on - strand at 4160.201 kb on - strand, within BWI76_RS19890 at 4160.349 kb on + strand, within BWI76_RS19890 at 4160.604 kb on - strand, within BWI76_RS19890 at 4160.724 kb on + strand at 4161.169 kb on + strand, within BWI76_RS19895 at 4161.170 kb on - strand, within BWI76_RS19895 at 4161.170 kb on - strand, within BWI76_RS19895
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,159,167 + BWI76_RS19885 0.47 +0.3 4,159,190 - BWI76_RS19885 0.48 +0.2 4,159,909 + +0.7 4,159,943 + -0.1 4,159,951 - +0.0 4,159,951 - -0.3 4,159,951 - +0.3 4,159,963 - -0.3 4,160,078 - -0.4 4,160,201 - BWI76_RS19890 0.14 +3.2 4,160,349 + BWI76_RS19890 0.36 +0.2 4,160,604 - BWI76_RS19890 0.75 +0.1 4,160,724 + +1.2 4,161,169 + BWI76_RS19895 0.38 -0.5 4,161,170 - BWI76_RS19895 0.38 -0.1 4,161,170 - BWI76_RS19895 0.38 +0.3
Or see this region's nucleotide sequence