Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19825 and BWI76_RS19830 are separated by 80 nucleotides BWI76_RS19830 and BWI76_RS19835 are separated by 50 nucleotides BWI76_RS19835 and BWI76_RS19840 are separated by 179 nucleotides BWI76_RS19840 and BWI76_RS19845 are separated by 19 nucleotides
BWI76_RS19825: BWI76_RS19825 - DNA mismatch repair protein MutT, at 4,146,193 to 4,146,786
_RS19825
BWI76_RS19830: BWI76_RS19830 - DeoR family transcriptional regulator, at 4,146,867 to 4,147,625
_RS19830
BWI76_RS19835: BWI76_RS19835 - nucleoid-associated protein YejK, at 4,147,676 to 4,148,683
_RS19835
BWI76_RS19840: BWI76_RS19840 - hypothetical protein, at 4,148,863 to 4,149,090
_RS19840
BWI76_RS19845: BWI76_RS19845 - phosphatase, at 4,149,110 to 4,150,870
_RS19845
Position (kb)
4147
4148
4149 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4146.691 kb on + strand, within BWI76_RS19825 at 4147.569 kb on - strand at 4147.625 kb on + strand at 4147.628 kb on - strand at 4147.628 kb on - strand at 4147.717 kb on + strand at 4147.977 kb on + strand, within BWI76_RS19835 at 4147.995 kb on - strand, within BWI76_RS19835 at 4148.466 kb on + strand, within BWI76_RS19835 at 4148.466 kb on + strand, within BWI76_RS19835 at 4148.474 kb on - strand, within BWI76_RS19835 at 4148.829 kb on - strand at 4148.885 kb on + strand at 4148.948 kb on + strand, within BWI76_RS19840
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,146,691 + BWI76_RS19825 0.84 -0.6 4,147,569 - -3.5 4,147,625 + +0.5 4,147,628 - -0.4 4,147,628 - +0.6 4,147,717 + -0.7 4,147,977 + BWI76_RS19835 0.30 +1.7 4,147,995 - BWI76_RS19835 0.32 +0.2 4,148,466 + BWI76_RS19835 0.78 +0.8 4,148,466 + BWI76_RS19835 0.78 -0.1 4,148,474 - BWI76_RS19835 0.79 +0.2 4,148,829 - -0.0 4,148,885 + -0.9 4,148,948 + BWI76_RS19840 0.37 -3.3
Or see this region's nucleotide sequence