Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18220 and BWI76_RS18225 are separated by 362 nucleotides BWI76_RS18225 and BWI76_RS18230 are separated by 28 nucleotides BWI76_RS18230 and BWI76_RS18235 overlap by 26 nucleotides BWI76_RS18235 and BWI76_RS18240 are separated by 159 nucleotides
BWI76_RS18220: BWI76_RS18220 - MFS transporter, at 3,785,287 to 3,786,870
_RS18220
BWI76_RS18225: BWI76_RS18225 - universal stress protein C, at 3,787,233 to 3,787,661
_RS18225
BWI76_RS18230: BWI76_RS18230 - trehalose-6-phosphate synthase, at 3,787,690 to 3,789,114
_RS18230
BWI76_RS18235: BWI76_RS18235 - trehalose-phosphatase, at 3,789,089 to 3,789,892
_RS18235
BWI76_RS18240: BWI76_RS18240 - L-arabinose ABC transporter permease AraH, at 3,790,052 to 3,791,032
_RS18240
Position (kb)
3787
3788
3789
3790 Strain fitness (log2 ratio)
-1
0
1 at 3787.542 kb on + strand, within BWI76_RS18225 at 3787.542 kb on + strand, within BWI76_RS18225 at 3787.567 kb on + strand, within BWI76_RS18225 at 3787.672 kb on + strand at 3788.197 kb on + strand, within BWI76_RS18230 at 3788.323 kb on - strand, within BWI76_RS18230 at 3788.400 kb on + strand, within BWI76_RS18230 at 3788.412 kb on + strand, within BWI76_RS18230 at 3789.109 kb on + strand at 3789.429 kb on + strand, within BWI76_RS18235 at 3789.542 kb on - strand, within BWI76_RS18235 at 3789.645 kb on - strand, within BWI76_RS18235 at 3789.754 kb on + strand, within BWI76_RS18235 at 3789.811 kb on - strand, within BWI76_RS18235 at 3789.961 kb on - strand at 3790.092 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,787,542 + BWI76_RS18225 0.72 +0.9 3,787,542 + BWI76_RS18225 0.72 +0.2 3,787,567 + BWI76_RS18225 0.78 -0.8 3,787,672 + -0.4 3,788,197 + BWI76_RS18230 0.36 +0.1 3,788,323 - BWI76_RS18230 0.44 +1.6 3,788,400 + BWI76_RS18230 0.50 +0.9 3,788,412 + BWI76_RS18230 0.51 -0.2 3,789,109 + -0.3 3,789,429 + BWI76_RS18235 0.42 +0.3 3,789,542 - BWI76_RS18235 0.56 -1.4 3,789,645 - BWI76_RS18235 0.69 -0.8 3,789,754 + BWI76_RS18235 0.83 -0.1 3,789,811 - BWI76_RS18235 0.90 +1.0 3,789,961 - +0.6 3,790,092 - -0.1
Or see this region's nucleotide sequence