Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16215 and BWI76_RS16220 overlap by 23 nucleotides BWI76_RS16220 and BWI76_RS16225 overlap by 1 nucleotides BWI76_RS16225 and BWI76_RS16230 are separated by 36 nucleotides BWI76_RS16230 and BWI76_RS16235 overlap by 4 nucleotides
BWI76_RS16215: BWI76_RS16215 - hypothetical protein, at 3,364,338 to 3,365,288
_RS16215
BWI76_RS16220: BWI76_RS16220 - hypothetical protein, at 3,365,266 to 3,365,468
_RS16220
BWI76_RS16225: BWI76_RS16225 - arginine:ornithine antiporter, at 3,365,468 to 3,366,850
_RS16225
BWI76_RS16230: BWI76_RS16230 - dihydromonapterin reductase, at 3,366,887 to 3,367,612
_RS16230
BWI76_RS16235: BWI76_RS16235 - membrane protein, at 3,367,609 to 3,367,944
_RS16235
Position (kb)
3365
3366
3367 Strain fitness (log2 ratio)
-1
0
1 at 3364.581 kb on - strand, within BWI76_RS16215 at 3364.813 kb on + strand, within BWI76_RS16215 at 3364.835 kb on + strand, within BWI76_RS16215 at 3365.240 kb on + strand at 3365.251 kb on - strand at 3365.402 kb on - strand, within BWI76_RS16220 at 3365.522 kb on - strand at 3365.686 kb on - strand at 3365.958 kb on - strand, within BWI76_RS16225 at 3366.062 kb on - strand, within BWI76_RS16225 at 3366.087 kb on - strand, within BWI76_RS16225 at 3366.160 kb on - strand, within BWI76_RS16225 at 3366.356 kb on + strand, within BWI76_RS16225 at 3366.356 kb on + strand, within BWI76_RS16225 at 3366.552 kb on + strand, within BWI76_RS16225 at 3367.111 kb on + strand, within BWI76_RS16230 at 3367.342 kb on - strand, within BWI76_RS16230 at 3367.527 kb on + strand at 3367.557 kb on - strand at 3367.647 kb on + strand, within BWI76_RS16235 at 3367.654 kb on + strand, within BWI76_RS16235 at 3367.816 kb on - strand, within BWI76_RS16235
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,364,581 - BWI76_RS16215 0.26 +0.4 3,364,813 + BWI76_RS16215 0.50 +0.1 3,364,835 + BWI76_RS16215 0.52 -0.5 3,365,240 + +0.1 3,365,251 - +0.3 3,365,402 - BWI76_RS16220 0.67 -0.0 3,365,522 - -0.7 3,365,686 - -1.0 3,365,958 - BWI76_RS16225 0.35 +1.1 3,366,062 - BWI76_RS16225 0.43 +0.4 3,366,087 - BWI76_RS16225 0.45 +0.5 3,366,160 - BWI76_RS16225 0.50 +0.4 3,366,356 + BWI76_RS16225 0.64 +0.2 3,366,356 + BWI76_RS16225 0.64 +0.4 3,366,552 + BWI76_RS16225 0.78 +0.5 3,367,111 + BWI76_RS16230 0.31 -0.5 3,367,342 - BWI76_RS16230 0.63 +0.5 3,367,527 + +0.4 3,367,557 - +0.5 3,367,647 + BWI76_RS16235 0.11 +0.1 3,367,654 + BWI76_RS16235 0.13 -0.7 3,367,816 - BWI76_RS16235 0.62 -0.3
Or see this region's nucleotide sequence