Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16150 and BWI76_RS16155 overlap by 4 nucleotides BWI76_RS16155 and BWI76_RS16160 are separated by 101 nucleotides BWI76_RS16160 and BWI76_RS16165 are separated by 166 nucleotides
BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290
_RS16150
BWI76_RS16155: BWI76_RS16155 - hypothetical protein, at 3,351,287 to 3,351,850
_RS16155
BWI76_RS16160: BWI76_RS16160 - LysR family transcriptional regulator, at 3,351,952 to 3,352,860
_RS16160
BWI76_RS16165: BWI76_RS16165 - peptidase M20, at 3,353,027 to 3,354,337
_RS16165
Position (kb)
3351
3352
3353 Strain fitness (log2 ratio)
-1
0
1 at 3351.301 kb on - strand at 3351.925 kb on + strand at 3351.929 kb on - strand at 3351.978 kb on - strand at 3352.211 kb on + strand, within BWI76_RS16160 at 3353.122 kb on + strand at 3353.211 kb on + strand, within BWI76_RS16165 at 3353.271 kb on - strand, within BWI76_RS16165 at 3353.544 kb on - strand, within BWI76_RS16165 at 3353.690 kb on + strand, within BWI76_RS16165 at 3353.833 kb on - strand, within BWI76_RS16165
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,351,301 - -0.6 3,351,925 + -0.2 3,351,929 - +0.3 3,351,978 - -0.0 3,352,211 + BWI76_RS16160 0.28 +0.3 3,353,122 + +0.9 3,353,211 + BWI76_RS16165 0.14 +0.0 3,353,271 - BWI76_RS16165 0.19 -0.1 3,353,544 - BWI76_RS16165 0.39 +0.5 3,353,690 + BWI76_RS16165 0.51 -0.2 3,353,833 - BWI76_RS16165 0.61 -0.4
Or see this region's nucleotide sequence