Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15320 and BWI76_RS15325 are separated by 9 nucleotides BWI76_RS15325 and BWI76_RS15330 overlap by 1 nucleotides BWI76_RS15330 and BWI76_RS15335 overlap by 1 nucleotides
BWI76_RS15320: BWI76_RS15320 - ABC transporter ATP-binding protein, at 3,208,339 to 3,209,037
_RS15320
BWI76_RS15325: BWI76_RS15325 - ABC transporter ATP-binding protein, at 3,209,047 to 3,209,844
_RS15325
BWI76_RS15330: BWI76_RS15330 - urea ABC transporter permease subunit UrtC, at 3,209,844 to 3,210,917
_RS15330
BWI76_RS15335: BWI76_RS15335 - branched-chain amino acid ABC transporter permease, at 3,210,917 to 3,212,491
_RS15335
Position (kb)
3209
3210
3211 Strain fitness (log2 ratio)
-1
0
1 at 3209.976 kb on + strand, within BWI76_RS15330 at 3209.976 kb on + strand, within BWI76_RS15330 at 3209.979 kb on - strand, within BWI76_RS15330 at 3210.145 kb on + strand, within BWI76_RS15330 at 3210.155 kb on + strand, within BWI76_RS15330 at 3210.155 kb on + strand at 3210.415 kb on + strand, within BWI76_RS15330 at 3210.925 kb on - strand at 3211.111 kb on + strand at 3211.144 kb on + strand, within BWI76_RS15335 at 3211.850 kb on + strand, within BWI76_RS15335 at 3211.885 kb on - strand, within BWI76_RS15335
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,209,976 + BWI76_RS15330 0.12 +0.3 3,209,976 + BWI76_RS15330 0.12 +1.2 3,209,979 - BWI76_RS15330 0.13 +0.6 3,210,145 + BWI76_RS15330 0.28 -1.5 3,210,155 + BWI76_RS15330 0.29 +0.7 3,210,155 + +1.0 3,210,415 + BWI76_RS15330 0.53 +0.0 3,210,925 - +0.2 3,211,111 + +0.0 3,211,144 + BWI76_RS15335 0.14 +0.1 3,211,850 + BWI76_RS15335 0.59 -0.3 3,211,885 - BWI76_RS15335 0.61 -0.7
Or see this region's nucleotide sequence