Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08125 and MMP_RS08130 are separated by 119 nucleotides MMP_RS08130 and MMP_RS08135 are separated by 181 nucleotides MMP_RS08135 and MMP_RS08140 are separated by 123 nucleotides
MMP_RS08125: MMP_RS08125 - dihydropteroate synthase-like protein, at 1,529,208 to 1,530,779
_RS08125
MMP_RS08130: MMP_RS08130 - IGHMBP2 family helicase, at 1,530,899 to 1,532,800
_RS08130
MMP_RS08135: MMP_RS08135 - arginine decarboxylase pyruvoyl-dependent, at 1,532,982 to 1,533,476
_RS08135
MMP_RS08140: MMP_RS08140 - adenosylmethionine decarboxylase, at 1,533,600 to 1,533,968
_RS08140
Position (kb)
1530
1531
1532
1533 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1530.003 kb on - strand at 1530.009 kb on + strand at 1530.351 kb on + strand at 1530.842 kb on - strand at 1530.861 kb on - strand at 1530.861 kb on - strand at 1530.861 kb on - strand at 1530.862 kb on + strand at 1530.862 kb on + strand at 1530.862 kb on + strand at 1530.862 kb on + strand at 1530.865 kb on - strand at 1530.865 kb on - strand at 1530.865 kb on - strand at 1530.865 kb on - strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.866 kb on + strand at 1530.867 kb on - strand at 1530.867 kb on - strand at 1530.868 kb on + strand at 1530.868 kb on + strand at 1530.880 kb on - strand at 1530.883 kb on - strand at 1530.883 kb on - strand at 1530.883 kb on - strand at 1531.032 kb on - strand at 1531.033 kb on + strand at 1531.038 kb on - strand at 1531.074 kb on + strand at 1531.074 kb on + strand at 1531.080 kb on - strand at 1531.143 kb on - strand, within MMP_RS08130 at 1531.148 kb on + strand, within MMP_RS08130 at 1531.341 kb on - strand, within MMP_RS08130 at 1531.341 kb on - strand, within MMP_RS08130 at 1531.341 kb on - strand, within MMP_RS08130 at 1531.342 kb on + strand, within MMP_RS08130 at 1531.342 kb on + strand, within MMP_RS08130 at 1531.345 kb on - strand, within MMP_RS08130 at 1531.464 kb on - strand, within MMP_RS08130 at 1531.586 kb on + strand, within MMP_RS08130 at 1531.593 kb on - strand, within MMP_RS08130 at 1531.594 kb on + strand, within MMP_RS08130 at 1531.673 kb on - strand, within MMP_RS08130 at 1531.723 kb on + strand, within MMP_RS08130 at 1532.119 kb on - strand, within MMP_RS08130 at 1532.289 kb on + strand, within MMP_RS08130 at 1532.297 kb on + strand, within MMP_RS08130 at 1532.520 kb on - strand, within MMP_RS08130 at 1532.520 kb on - strand, within MMP_RS08130 at 1532.524 kb on + strand, within MMP_RS08130 at 1532.549 kb on - strand, within MMP_RS08130 at 1532.677 kb on - strand at 1532.722 kb on + strand at 1532.792 kb on + strand at 1533.132 kb on + strand at 1533.479 kb on - strand at 1533.480 kb on + strand at 1533.481 kb on - strand at 1533.483 kb on - strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.500 kb on - strand at 1533.501 kb on + strand at 1533.501 kb on + strand at 1533.501 kb on + strand at 1533.506 kb on + strand at 1533.517 kb on - strand at 1533.518 kb on + strand at 1533.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,530,003 - -1.9 1,530,009 + -2.4 1,530,351 + -2.9 1,530,842 - +0.6 1,530,861 - +0.7 1,530,861 - -1.2 1,530,861 - +0.6 1,530,862 + -0.9 1,530,862 + -1.6 1,530,862 + -1.7 1,530,862 + -1.1 1,530,865 - +1.2 1,530,865 - -0.2 1,530,865 - +0.6 1,530,865 - -0.6 1,530,866 + -0.4 1,530,866 + +0.4 1,530,866 + -0.6 1,530,866 + +0.1 1,530,866 + -1.0 1,530,866 + -1.4 1,530,866 + +0.3 1,530,866 + -1.2 1,530,866 + -0.6 1,530,867 - +0.0 1,530,867 - +0.4 1,530,868 + -1.6 1,530,868 + -1.2 1,530,880 - +1.2 1,530,883 - -0.3 1,530,883 - -0.1 1,530,883 - +0.4 1,531,032 - +1.0 1,531,033 + +0.7 1,531,038 - +1.0 1,531,074 + -0.2 1,531,074 + +0.4 1,531,080 - -1.0 1,531,143 - MMP_RS08130 0.13 +1.0 1,531,148 + MMP_RS08130 0.13 +0.8 1,531,341 - MMP_RS08130 0.23 +0.6 1,531,341 - MMP_RS08130 0.23 +0.1 1,531,341 - MMP_RS08130 0.23 +0.5 1,531,342 + MMP_RS08130 0.23 -0.5 1,531,342 + MMP_RS08130 0.23 +0.4 1,531,345 - MMP_RS08130 0.23 +1.2 1,531,464 - MMP_RS08130 0.30 +0.6 1,531,586 + MMP_RS08130 0.36 +1.0 1,531,593 - MMP_RS08130 0.36 +0.4 1,531,594 + MMP_RS08130 0.37 -0.5 1,531,673 - MMP_RS08130 0.41 +0.4 1,531,723 + MMP_RS08130 0.43 +1.4 1,532,119 - MMP_RS08130 0.64 +0.4 1,532,289 + MMP_RS08130 0.73 +0.2 1,532,297 + MMP_RS08130 0.74 +0.8 1,532,520 - MMP_RS08130 0.85 +0.7 1,532,520 - MMP_RS08130 0.85 +0.6 1,532,524 + MMP_RS08130 0.85 +1.0 1,532,549 - MMP_RS08130 0.87 +0.8 1,532,677 - +1.0 1,532,722 + +0.0 1,532,792 + +0.8 1,533,132 + -1.9 1,533,479 - +1.3 1,533,480 + +0.2 1,533,481 - +0.8 1,533,483 - +1.4 1,533,484 + +1.5 1,533,484 + +1.9 1,533,484 + +1.0 1,533,484 + +0.7 1,533,484 + +1.7 1,533,500 - +1.2 1,533,501 + +0.9 1,533,501 + +1.7 1,533,501 + +1.6 1,533,506 + +0.8 1,533,517 - +0.8 1,533,518 + +1.6 1,533,518 + +1.5
Or see this region's nucleotide sequence