Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07960 and MMP_RS07965 are separated by 21 nucleotides MMP_RS07965 and MMP_RS07970 overlap by 4 nucleotides MMP_RS07970 and MMP_RS07975 are separated by 138 nucleotides
MMP_RS07960: MMP_RS07960 - sugar phosphate isomerase/epimerase, at 1,502,975 to 1,503,781
_RS07960
MMP_RS07965: MMP_RS07965 - NADPH-dependent F420 reductase, at 1,503,803 to 1,504,474
_RS07965
MMP_RS07970: MMP_RS07970 - hypothetical protein, at 1,504,471 to 1,504,692
_RS07970
MMP_RS07975: MMP_RS07975 - signal recognition particle protein Srp54, at 1,504,831 to 1,506,183
_RS07975
Position (kb)
1504
1505 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1503.494 kb on + strand, within MMP_RS07960 at 1503.680 kb on + strand, within MMP_RS07960 at 1503.683 kb on + strand, within MMP_RS07960 at 1503.686 kb on - strand, within MMP_RS07960 at 1503.686 kb on - strand, within MMP_RS07960 at 1503.687 kb on + strand, within MMP_RS07960 at 1503.759 kb on + strand at 1504.038 kb on + strand at 1504.092 kb on - strand at 1504.465 kb on + strand at 1504.472 kb on - strand at 1504.548 kb on - strand, within MMP_RS07970 at 1504.577 kb on + strand, within MMP_RS07970 at 1504.683 kb on - strand at 1504.724 kb on + strand at 1504.755 kb on + strand at 1504.781 kb on + strand at 1504.817 kb on + strand at 1504.817 kb on + strand at 1504.841 kb on - strand at 1504.841 kb on - strand at 1504.842 kb on + strand at 1505.287 kb on - strand at 1505.287 kb on - strand at 1505.341 kb on - strand at 1505.342 kb on + strand at 1505.368 kb on - strand at 1505.437 kb on - strand at 1505.630 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,503,494 + MMP_RS07960 0.64 +0.3 1,503,680 + MMP_RS07960 0.87 +1.5 1,503,683 + MMP_RS07960 0.88 +0.4 1,503,686 - MMP_RS07960 0.88 +0.3 1,503,686 - MMP_RS07960 0.88 +1.1 1,503,687 + MMP_RS07960 0.88 +0.5 1,503,759 + -0.1 1,504,038 + -1.2 1,504,092 - -2.5 1,504,465 + +1.1 1,504,472 - +0.7 1,504,548 - MMP_RS07970 0.35 +0.5 1,504,577 + MMP_RS07970 0.48 +0.5 1,504,683 - -1.6 1,504,724 + +0.3 1,504,755 + +0.8 1,504,781 + +0.9 1,504,817 + -0.1 1,504,817 + +1.6 1,504,841 - +0.1 1,504,841 - +0.7 1,504,842 + +0.3 1,505,287 - -2.1 1,505,287 - -2.1 1,505,341 - -1.5 1,505,342 + -2.9 1,505,368 - -1.1 1,505,437 - -2.8 1,505,630 + -4.0
Or see this region's nucleotide sequence