Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07550 and MMP_RS07555 are separated by 54 nucleotides MMP_RS07555 and MMP_RS07560 are separated by 173 nucleotides MMP_RS07560 and MMP_RS07565 are separated by 129 nucleotides MMP_RS07565 and MMP_RS07570 are separated by 101 nucleotides
MMP_RS07550: MMP_RS07550 - L-serine ammonia-lyase iron-sulfur-dependent subunit alpha, at 1,428,750 to 1,429,940
_RS07550
MMP_RS07555: MMP_RS07555 - monovalent cation/H+ antiporter subunit E, at 1,429,995 to 1,430,315
_RS07555
MMP_RS07560: MMP_RS07560 - prefoldin subunit alpha, at 1,430,489 to 1,430,923
_RS07560
MMP_RS07565: MMP_RS07565 - tRNA-Val, at 1,431,053 to 1,431,129
_RS07565
MMP_RS07570: MMP_RS07570 - nucleotidyltransferase family protein, at 1,431,231 to 1,432,280
_RS07570
Position (kb)
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1 at 1429.494 kb on + strand, within MMP_RS07550 at 1429.641 kb on - strand, within MMP_RS07550 at 1429.661 kb on - strand, within MMP_RS07550 at 1429.714 kb on + strand, within MMP_RS07550 at 1429.743 kb on + strand, within MMP_RS07550 at 1429.784 kb on - strand, within MMP_RS07550 at 1429.917 kb on - strand at 1429.940 kb on - strand at 1429.978 kb on + strand at 1430.013 kb on - strand at 1430.017 kb on - strand at 1430.018 kb on + strand at 1430.068 kb on + strand, within MMP_RS07555 at 1430.350 kb on - strand at 1430.354 kb on - strand at 1430.355 kb on + strand at 1430.356 kb on - strand at 1430.356 kb on - strand at 1430.358 kb on - strand at 1430.359 kb on + strand at 1430.359 kb on + strand at 1430.359 kb on + strand at 1430.360 kb on - strand at 1430.360 kb on - strand at 1430.361 kb on + strand at 1430.361 kb on + strand at 1430.364 kb on - strand at 1430.452 kb on - strand at 1430.453 kb on + strand at 1430.456 kb on - strand at 1430.456 kb on - strand at 1430.555 kb on + strand, within MMP_RS07560 at 1430.745 kb on - strand, within MMP_RS07560 at 1430.746 kb on + strand, within MMP_RS07560 at 1430.746 kb on + strand, within MMP_RS07560 at 1430.809 kb on + strand, within MMP_RS07560 at 1430.809 kb on + strand, within MMP_RS07560 at 1430.921 kb on - strand at 1430.973 kb on + strand at 1431.043 kb on + strand at 1431.503 kb on - strand, within MMP_RS07570 at 1431.683 kb on + strand, within MMP_RS07570 at 1431.729 kb on + strand, within MMP_RS07570 at 1431.729 kb on + strand, within MMP_RS07570 at 1431.731 kb on - strand, within MMP_RS07570 at 1431.731 kb on - strand, within MMP_RS07570 at 1431.786 kb on - strand, within MMP_RS07570 at 1431.787 kb on + strand, within MMP_RS07570 at 1431.787 kb on + strand, within MMP_RS07570 at 1431.807 kb on - strand, within MMP_RS07570 at 1431.807 kb on - strand, within MMP_RS07570
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,429,494 + MMP_RS07550 0.62 +0.3 1,429,641 - MMP_RS07550 0.75 -0.0 1,429,661 - MMP_RS07550 0.76 +1.4 1,429,714 + MMP_RS07550 0.81 +0.2 1,429,743 + MMP_RS07550 0.83 +0.6 1,429,784 - MMP_RS07550 0.87 +0.9 1,429,917 - +0.6 1,429,940 - -0.3 1,429,978 + -0.3 1,430,013 - +0.4 1,430,017 - -1.0 1,430,018 + +0.2 1,430,068 + MMP_RS07555 0.23 -1.7 1,430,350 - -0.7 1,430,354 - -0.6 1,430,355 + -1.9 1,430,356 - -0.3 1,430,356 - -0.3 1,430,358 - -0.2 1,430,359 + -0.8 1,430,359 + -1.5 1,430,359 + +0.1 1,430,360 - -0.9 1,430,360 - -0.3 1,430,361 + +0.6 1,430,361 + -0.9 1,430,364 - +0.6 1,430,452 - -0.9 1,430,453 + +0.8 1,430,456 - -0.5 1,430,456 - +0.6 1,430,555 + MMP_RS07560 0.15 +0.2 1,430,745 - MMP_RS07560 0.59 +0.4 1,430,746 + MMP_RS07560 0.59 +0.4 1,430,746 + MMP_RS07560 0.59 +0.2 1,430,809 + MMP_RS07560 0.74 +1.2 1,430,809 + MMP_RS07560 0.74 +1.6 1,430,921 - +0.8 1,430,973 + +0.3 1,431,043 + -0.1 1,431,503 - MMP_RS07570 0.26 -0.0 1,431,683 + MMP_RS07570 0.43 -1.3 1,431,729 + MMP_RS07570 0.47 +0.8 1,431,729 + MMP_RS07570 0.47 -0.7 1,431,731 - MMP_RS07570 0.48 +1.5 1,431,731 - MMP_RS07570 0.48 +1.6 1,431,786 - MMP_RS07570 0.53 -1.3 1,431,787 + MMP_RS07570 0.53 +0.7 1,431,787 + MMP_RS07570 0.53 +0.7 1,431,807 - MMP_RS07570 0.55 -0.4 1,431,807 - MMP_RS07570 0.55 -0.3
Or see this region's nucleotide sequence