Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06855 and MMP_RS06860 are separated by 47 nucleotides MMP_RS06860 and MMP_RS06865 are separated by 25 nucleotides MMP_RS06865 and MMP_RS06870 are separated by 96 nucleotides MMP_RS06870 and MMP_RS06875 are separated by 38 nucleotides
MMP_RS06855: MMP_RS06855 - hypothetical protein, at 1,309,768 to 1,310,703
_RS06855
MMP_RS06860: MMP_RS06860 - 4Fe-4S binding protein, at 1,310,751 to 1,310,939
_RS06860
MMP_RS06865: MMP_RS06865 - HypC/HybG/HupF family hydrogenase formation chaperone, at 1,310,965 to 1,311,231
_RS06865
MMP_RS06870: MMP_RS06870 - DedA family protein, at 1,311,328 to 1,311,801
_RS06870
MMP_RS06875: MMP_RS06875 - FAD-dependent oxidoreductase, at 1,311,840 to 1,312,994
_RS06875
Position (kb)
1311
1312 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1310.374 kb on + strand, within MMP_RS06855 at 1310.402 kb on - strand, within MMP_RS06855 at 1310.519 kb on + strand, within MMP_RS06855 at 1310.521 kb on - strand, within MMP_RS06855 at 1310.591 kb on - strand, within MMP_RS06855 at 1310.592 kb on + strand, within MMP_RS06855 at 1310.632 kb on + strand at 1310.691 kb on - strand at 1310.721 kb on - strand at 1310.722 kb on + strand at 1310.723 kb on - strand at 1310.723 kb on - strand at 1310.724 kb on + strand at 1310.730 kb on - strand at 1310.851 kb on - strand, within MMP_RS06860 at 1310.881 kb on - strand, within MMP_RS06860 at 1310.937 kb on - strand at 1310.941 kb on - strand at 1311.007 kb on - strand, within MMP_RS06865 at 1311.098 kb on - strand, within MMP_RS06865 at 1311.099 kb on + strand, within MMP_RS06865 at 1311.209 kb on + strand at 1311.258 kb on - strand at 1311.267 kb on - strand at 1311.273 kb on - strand at 1311.295 kb on + strand at 1311.343 kb on + strand at 1311.435 kb on - strand, within MMP_RS06870 at 1311.480 kb on - strand, within MMP_RS06870 at 1311.517 kb on + strand, within MMP_RS06870 at 1311.543 kb on + strand, within MMP_RS06870 at 1311.556 kb on + strand, within MMP_RS06870 at 1311.556 kb on + strand, within MMP_RS06870 at 1311.602 kb on - strand, within MMP_RS06870 at 1311.608 kb on + strand, within MMP_RS06870 at 1311.765 kb on - strand at 1311.765 kb on - strand at 1311.769 kb on + strand at 1311.802 kb on + strand at 1311.806 kb on - strand at 1311.826 kb on + strand at 1311.841 kb on - strand at 1311.913 kb on + strand at 1311.917 kb on + strand at 1311.958 kb on + strand, within MMP_RS06875 at 1312.100 kb on - strand, within MMP_RS06875 at 1312.119 kb on + strand, within MMP_RS06875 at 1312.161 kb on + strand, within MMP_RS06875 at 1312.172 kb on - strand, within MMP_RS06875 at 1312.326 kb on + strand, within MMP_RS06875 at 1312.462 kb on - strand, within MMP_RS06875 at 1312.783 kb on + strand, within MMP_RS06875 at 1312.792 kb on + strand, within MMP_RS06875 at 1312.792 kb on + strand, within MMP_RS06875
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,310,374 + MMP_RS06855 0.65 -1.4 1,310,402 - MMP_RS06855 0.68 -2.3 1,310,519 + MMP_RS06855 0.80 -2.0 1,310,521 - MMP_RS06855 0.80 -2.3 1,310,591 - MMP_RS06855 0.88 -3.1 1,310,592 + MMP_RS06855 0.88 -2.1 1,310,632 + -2.0 1,310,691 - -1.3 1,310,721 - +0.1 1,310,722 + +0.2 1,310,723 - +1.1 1,310,723 - +0.7 1,310,724 + -0.3 1,310,730 - -0.1 1,310,851 - MMP_RS06860 0.53 +1.1 1,310,881 - MMP_RS06860 0.69 +0.8 1,310,937 - +0.4 1,310,941 - +1.1 1,311,007 - MMP_RS06865 0.16 +0.2 1,311,098 - MMP_RS06865 0.50 -2.4 1,311,099 + MMP_RS06865 0.50 -0.7 1,311,209 + -3.2 1,311,258 - -2.4 1,311,267 - +0.8 1,311,273 - +0.7 1,311,295 + +0.2 1,311,343 + -0.6 1,311,435 - MMP_RS06870 0.23 +1.1 1,311,480 - MMP_RS06870 0.32 +0.6 1,311,517 + MMP_RS06870 0.40 +0.8 1,311,543 + MMP_RS06870 0.45 +0.4 1,311,556 + MMP_RS06870 0.48 +1.8 1,311,556 + MMP_RS06870 0.48 +1.5 1,311,602 - MMP_RS06870 0.58 +1.0 1,311,608 + MMP_RS06870 0.59 +0.4 1,311,765 - +1.1 1,311,765 - +1.0 1,311,769 + +0.3 1,311,802 + +0.7 1,311,806 - +0.3 1,311,826 + +1.2 1,311,841 - -0.2 1,311,913 + +0.8 1,311,917 + +1.0 1,311,958 + MMP_RS06875 0.10 -1.0 1,312,100 - MMP_RS06875 0.23 +0.6 1,312,119 + MMP_RS06875 0.24 +1.7 1,312,161 + MMP_RS06875 0.28 -0.2 1,312,172 - MMP_RS06875 0.29 -0.3 1,312,326 + MMP_RS06875 0.42 +0.3 1,312,462 - MMP_RS06875 0.54 -1.5 1,312,783 + MMP_RS06875 0.82 +0.8 1,312,792 + MMP_RS06875 0.82 +0.6 1,312,792 + MMP_RS06875 0.82 -2.1
Or see this region's nucleotide sequence