Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06870

Experiment: H2 N-free with 10mM Glycine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06855 and MMP_RS06860 are separated by 47 nucleotidesMMP_RS06860 and MMP_RS06865 are separated by 25 nucleotidesMMP_RS06865 and MMP_RS06870 are separated by 96 nucleotidesMMP_RS06870 and MMP_RS06875 are separated by 38 nucleotides MMP_RS06855: MMP_RS06855 - hypothetical protein, at 1,309,768 to 1,310,703 _RS06855 MMP_RS06860: MMP_RS06860 - 4Fe-4S binding protein, at 1,310,751 to 1,310,939 _RS06860 MMP_RS06865: MMP_RS06865 - HypC/HybG/HupF family hydrogenase formation chaperone, at 1,310,965 to 1,311,231 _RS06865 MMP_RS06870: MMP_RS06870 - DedA family protein, at 1,311,328 to 1,311,801 _RS06870 MMP_RS06875: MMP_RS06875 - FAD-dependent oxidoreductase, at 1,311,840 to 1,312,994 _RS06875 Position (kb) 1311 1312Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1310.374 kb on + strand, within MMP_RS06855at 1310.402 kb on - strand, within MMP_RS06855at 1310.519 kb on + strand, within MMP_RS06855at 1310.521 kb on - strand, within MMP_RS06855at 1310.591 kb on - strand, within MMP_RS06855at 1310.592 kb on + strand, within MMP_RS06855at 1310.632 kb on + strandat 1310.691 kb on - strandat 1310.721 kb on - strandat 1310.722 kb on + strandat 1310.723 kb on - strandat 1310.723 kb on - strandat 1310.724 kb on + strandat 1310.730 kb on - strandat 1310.851 kb on - strand, within MMP_RS06860at 1310.881 kb on - strand, within MMP_RS06860at 1310.937 kb on - strandat 1310.941 kb on - strandat 1311.007 kb on - strand, within MMP_RS06865at 1311.098 kb on - strand, within MMP_RS06865at 1311.099 kb on + strand, within MMP_RS06865at 1311.209 kb on + strandat 1311.258 kb on - strandat 1311.267 kb on - strandat 1311.273 kb on - strandat 1311.295 kb on + strandat 1311.343 kb on + strandat 1311.435 kb on - strand, within MMP_RS06870at 1311.480 kb on - strand, within MMP_RS06870at 1311.517 kb on + strand, within MMP_RS06870at 1311.543 kb on + strand, within MMP_RS06870at 1311.556 kb on + strand, within MMP_RS06870at 1311.556 kb on + strand, within MMP_RS06870at 1311.602 kb on - strand, within MMP_RS06870at 1311.608 kb on + strand, within MMP_RS06870at 1311.765 kb on - strandat 1311.765 kb on - strandat 1311.769 kb on + strandat 1311.802 kb on + strandat 1311.806 kb on - strandat 1311.826 kb on + strandat 1311.841 kb on - strandat 1311.913 kb on + strandat 1311.917 kb on + strandat 1311.958 kb on + strand, within MMP_RS06875at 1312.100 kb on - strand, within MMP_RS06875at 1312.119 kb on + strand, within MMP_RS06875at 1312.161 kb on + strand, within MMP_RS06875at 1312.172 kb on - strand, within MMP_RS06875at 1312.326 kb on + strand, within MMP_RS06875at 1312.462 kb on - strand, within MMP_RS06875at 1312.783 kb on + strand, within MMP_RS06875at 1312.792 kb on + strand, within MMP_RS06875at 1312.792 kb on + strand, within MMP_RS06875

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine
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1,310,374 + MMP_RS06855 0.65 -1.4
1,310,402 - MMP_RS06855 0.68 -2.3
1,310,519 + MMP_RS06855 0.80 -2.0
1,310,521 - MMP_RS06855 0.80 -2.3
1,310,591 - MMP_RS06855 0.88 -3.1
1,310,592 + MMP_RS06855 0.88 -2.1
1,310,632 + -2.0
1,310,691 - -1.3
1,310,721 - +0.1
1,310,722 + +0.2
1,310,723 - +1.1
1,310,723 - +0.7
1,310,724 + -0.3
1,310,730 - -0.1
1,310,851 - MMP_RS06860 0.53 +1.1
1,310,881 - MMP_RS06860 0.69 +0.8
1,310,937 - +0.4
1,310,941 - +1.1
1,311,007 - MMP_RS06865 0.16 +0.2
1,311,098 - MMP_RS06865 0.50 -2.4
1,311,099 + MMP_RS06865 0.50 -0.7
1,311,209 + -3.2
1,311,258 - -2.4
1,311,267 - +0.8
1,311,273 - +0.7
1,311,295 + +0.2
1,311,343 + -0.6
1,311,435 - MMP_RS06870 0.23 +1.1
1,311,480 - MMP_RS06870 0.32 +0.6
1,311,517 + MMP_RS06870 0.40 +0.8
1,311,543 + MMP_RS06870 0.45 +0.4
1,311,556 + MMP_RS06870 0.48 +1.8
1,311,556 + MMP_RS06870 0.48 +1.5
1,311,602 - MMP_RS06870 0.58 +1.0
1,311,608 + MMP_RS06870 0.59 +0.4
1,311,765 - +1.1
1,311,765 - +1.0
1,311,769 + +0.3
1,311,802 + +0.7
1,311,806 - +0.3
1,311,826 + +1.2
1,311,841 - -0.2
1,311,913 + +0.8
1,311,917 + +1.0
1,311,958 + MMP_RS06875 0.10 -1.0
1,312,100 - MMP_RS06875 0.23 +0.6
1,312,119 + MMP_RS06875 0.24 +1.7
1,312,161 + MMP_RS06875 0.28 -0.2
1,312,172 - MMP_RS06875 0.29 -0.3
1,312,326 + MMP_RS06875 0.42 +0.3
1,312,462 - MMP_RS06875 0.54 -1.5
1,312,783 + MMP_RS06875 0.82 +0.8
1,312,792 + MMP_RS06875 0.82 +0.6
1,312,792 + MMP_RS06875 0.82 -2.1

Or see this region's nucleotide sequence