Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06335 and MMP_RS06340 are separated by 20 nucleotides MMP_RS06340 and MMP_RS06345 are separated by 52 nucleotides MMP_RS06345 and MMP_RS06350 are separated by 79 nucleotides MMP_RS06350 and MMP_RS06355 overlap by 8 nucleotides
MMP_RS06335: MMP_RS06335 - HAD family hydrolase, at 1,219,526 to 1,219,909
_RS06335
MMP_RS06340: MMP_RS06340 - phosphoadenosine phosphosulfate reductase family protein, at 1,219,930 to 1,220,820
_RS06340
MMP_RS06345: MMP_RS06345 - formate dehydrogenase accessory sulfurtransferase FdhD, at 1,220,873 to 1,221,574
_RS06345
MMP_RS06350: MMP_RS06350 - HesA/MoeB/ThiF family protein, at 1,221,654 to 1,222,373
_RS06350
MMP_RS06355: MMP_RS06355 - molybdenum cofactor biosynthesis protein MoaE, at 1,222,366 to 1,222,791
_RS06355
Position (kb)
1220
1221
1222 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1219.987 kb on - strand at 1220.075 kb on + strand, within MMP_RS06340 at 1220.173 kb on - strand, within MMP_RS06340 at 1220.211 kb on - strand, within MMP_RS06340 at 1220.326 kb on + strand, within MMP_RS06340 at 1220.352 kb on + strand, within MMP_RS06340 at 1220.359 kb on - strand, within MMP_RS06340 at 1220.404 kb on - strand, within MMP_RS06340 at 1220.455 kb on + strand, within MMP_RS06340 at 1220.460 kb on - strand, within MMP_RS06340 at 1220.523 kb on - strand, within MMP_RS06340 at 1220.524 kb on + strand, within MMP_RS06340 at 1220.548 kb on + strand, within MMP_RS06340 at 1220.714 kb on + strand, within MMP_RS06340 at 1220.761 kb on - strand at 1220.822 kb on - strand at 1220.847 kb on + strand at 1220.857 kb on + strand at 1220.888 kb on - strand at 1220.898 kb on + strand at 1220.907 kb on - strand at 1220.914 kb on - strand at 1221.002 kb on + strand, within MMP_RS06345 at 1221.004 kb on - strand, within MMP_RS06345 at 1221.268 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.334 kb on + strand, within MMP_RS06345 at 1221.334 kb on + strand, within MMP_RS06345 at 1221.334 kb on + strand, within MMP_RS06345 at 1221.336 kb on + strand, within MMP_RS06345 at 1221.351 kb on + strand, within MMP_RS06345 at 1221.356 kb on + strand, within MMP_RS06345 at 1221.384 kb on - strand, within MMP_RS06345 at 1221.384 kb on - strand, within MMP_RS06345 at 1221.543 kb on - strand at 1221.599 kb on + strand at 1221.610 kb on - strand at 1221.610 kb on - strand at 1221.611 kb on + strand at 1221.628 kb on + strand at 1221.630 kb on + strand at 1221.641 kb on - strand at 1221.641 kb on - strand at 1221.642 kb on + strand at 1221.642 kb on + strand at 1221.654 kb on + strand at 1221.654 kb on + strand at 1221.654 kb on + strand at 1221.781 kb on + strand, within MMP_RS06350 at 1221.860 kb on + strand, within MMP_RS06350 at 1221.927 kb on + strand, within MMP_RS06350 at 1222.030 kb on + strand, within MMP_RS06350 at 1222.041 kb on + strand, within MMP_RS06350 at 1222.053 kb on + strand, within MMP_RS06350 at 1222.156 kb on - strand, within MMP_RS06350
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,219,987 - -1.6 1,220,075 + MMP_RS06340 0.16 -1.5 1,220,173 - MMP_RS06340 0.27 -0.7 1,220,211 - MMP_RS06340 0.32 -0.5 1,220,326 + MMP_RS06340 0.44 -2.2 1,220,352 + MMP_RS06340 0.47 -1.2 1,220,359 - MMP_RS06340 0.48 -1.2 1,220,404 - MMP_RS06340 0.53 -0.6 1,220,455 + MMP_RS06340 0.59 -1.2 1,220,460 - MMP_RS06340 0.59 +0.2 1,220,523 - MMP_RS06340 0.67 -3.4 1,220,524 + MMP_RS06340 0.67 -1.3 1,220,548 + MMP_RS06340 0.69 -1.3 1,220,714 + MMP_RS06340 0.88 -0.2 1,220,761 - -0.4 1,220,822 - -2.0 1,220,847 + +0.5 1,220,857 + +0.2 1,220,888 - +0.7 1,220,898 + +1.6 1,220,907 - +0.3 1,220,914 - +0.6 1,221,002 + MMP_RS06345 0.18 +0.3 1,221,004 - MMP_RS06345 0.19 +0.7 1,221,268 - MMP_RS06345 0.56 +0.6 1,221,333 - MMP_RS06345 0.66 -0.2 1,221,333 - MMP_RS06345 0.66 -0.5 1,221,333 - MMP_RS06345 0.66 +0.5 1,221,333 - MMP_RS06345 0.66 +0.9 1,221,333 - MMP_RS06345 0.66 +0.6 1,221,333 - MMP_RS06345 0.66 +1.7 1,221,334 + MMP_RS06345 0.66 +0.8 1,221,334 + MMP_RS06345 0.66 +1.0 1,221,334 + MMP_RS06345 0.66 +1.2 1,221,336 + MMP_RS06345 0.66 +0.4 1,221,351 + MMP_RS06345 0.68 +0.8 1,221,356 + MMP_RS06345 0.69 +0.3 1,221,384 - MMP_RS06345 0.73 +0.9 1,221,384 - MMP_RS06345 0.73 +0.5 1,221,543 - -0.1 1,221,599 + +1.2 1,221,610 - -0.1 1,221,610 - +0.3 1,221,611 + -1.8 1,221,628 + -0.4 1,221,630 + +0.7 1,221,641 - +0.4 1,221,641 - -0.8 1,221,642 + +0.8 1,221,642 + +0.1 1,221,654 + -1.2 1,221,654 + -2.9 1,221,654 + -2.2 1,221,781 + MMP_RS06350 0.18 -3.1 1,221,860 + MMP_RS06350 0.29 -2.7 1,221,927 + MMP_RS06350 0.38 -3.3 1,222,030 + MMP_RS06350 0.52 -4.0 1,222,041 + MMP_RS06350 0.54 -0.3 1,222,053 + MMP_RS06350 0.55 -3.4 1,222,156 - MMP_RS06350 0.70 -3.2
Or see this region's nucleotide sequence