Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06315 and MMP_RS06320 are separated by 22 nucleotides MMP_RS06320 and MMP_RS06325 are separated by 94 nucleotides MMP_RS06325 and MMP_RS06330 are separated by 72 nucleotides
MMP_RS06315: MMP_RS06315 - cobalt-precorrin-7 (C(5))-methyltransferase, at 1,216,616 to 1,217,242
_RS06315
MMP_RS06320: MMP_RS06320 - hypothetical protein, at 1,217,265 to 1,217,903
_RS06320
MMP_RS06325: MMP_RS06325 - hypothetical protein, at 1,217,998 to 1,218,300
_RS06325
MMP_RS06330: MMP_RS06330 - nucleotidyltransferase domain-containing protein, at 1,218,373 to 1,219,431
_RS06330
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1216.998 kb on - strand, within MMP_RS06315 at 1216.999 kb on + strand, within MMP_RS06315 at 1217.233 kb on - strand at 1217.236 kb on + strand at 1217.386 kb on - strand, within MMP_RS06320 at 1217.391 kb on + strand, within MMP_RS06320 at 1217.396 kb on - strand, within MMP_RS06320 at 1217.396 kb on - strand, within MMP_RS06320 at 1217.397 kb on + strand, within MMP_RS06320 at 1217.401 kb on - strand, within MMP_RS06320 at 1217.472 kb on - strand, within MMP_RS06320 at 1217.612 kb on - strand, within MMP_RS06320 at 1217.826 kb on - strand, within MMP_RS06320 at 1217.864 kb on - strand at 1217.901 kb on - strand at 1217.963 kb on - strand at 1217.966 kb on - strand at 1217.992 kb on - strand at 1217.993 kb on + strand at 1218.004 kb on + strand at 1218.005 kb on - strand at 1218.070 kb on + strand, within MMP_RS06325 at 1218.313 kb on - strand at 1218.314 kb on + strand at 1218.316 kb on + strand at 1218.316 kb on + strand at 1218.319 kb on - strand at 1218.320 kb on + strand at 1218.320 kb on + strand at 1218.384 kb on - strand at 1218.476 kb on - strand at 1218.536 kb on - strand at 1219.097 kb on - strand at 1219.123 kb on - strand at 1219.291 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,216,998 - MMP_RS06315 0.61 -2.4 1,216,999 + MMP_RS06315 0.61 -3.8 1,217,233 - -4.0 1,217,236 + -2.5 1,217,386 - MMP_RS06320 0.19 +0.1 1,217,391 + MMP_RS06320 0.20 +1.7 1,217,396 - MMP_RS06320 0.21 -0.4 1,217,396 - MMP_RS06320 0.21 +0.6 1,217,397 + MMP_RS06320 0.21 -1.3 1,217,401 - MMP_RS06320 0.21 +0.3 1,217,472 - MMP_RS06320 0.32 +0.4 1,217,612 - MMP_RS06320 0.54 +1.0 1,217,826 - MMP_RS06320 0.88 +0.3 1,217,864 - +0.9 1,217,901 - +1.3 1,217,963 - -1.6 1,217,966 - +0.3 1,217,992 - -1.6 1,217,993 + +0.4 1,218,004 + +0.8 1,218,005 - -2.4 1,218,070 + MMP_RS06325 0.24 +0.7 1,218,313 - +0.1 1,218,314 + -0.6 1,218,316 + +0.5 1,218,316 + -0.5 1,218,319 - -0.7 1,218,320 + +0.1 1,218,320 + +0.1 1,218,384 - -4.0 1,218,476 - -2.8 1,218,536 - -3.9 1,219,097 - -6.1 1,219,123 - -4.4 1,219,291 - -3.7
Or see this region's nucleotide sequence