Strain Fitness in Methanococcus maripaludis S2 around MMP_RS06300

Experiment: H2 N-free with 10mM Glycine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS06295 and MMP_RS06300 are separated by 289 nucleotidesMMP_RS06300 and MMP_RS06305 are separated by 115 nucleotidesMMP_RS06305 and MMP_RS06310 are separated by 19 nucleotides MMP_RS06295: MMP_RS06295 - methanogenesis marker 14 protein, at 1,211,914 to 1,213,392 _RS06295 MMP_RS06300: MMP_RS06300 - ABC transporter substrate-binding protein, at 1,213,682 to 1,214,446 _RS06300 MMP_RS06305: MMP_RS06305 - transporter substrate-binding domain-containing protein, at 1,214,562 to 1,215,326 _RS06305 MMP_RS06310: MMP_RS06310 - NAD(P)/FAD-dependent oxidoreductase, at 1,215,346 to 1,216,503 _RS06310 Position (kb) 1213 1214 1215Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1212.791 kb on + strand, within MMP_RS06295at 1212.947 kb on + strand, within MMP_RS06295at 1213.158 kb on - strand, within MMP_RS06295at 1213.174 kb on - strand, within MMP_RS06295at 1213.174 kb on - strand, within MMP_RS06295at 1213.394 kb on - strandat 1213.408 kb on + strandat 1213.413 kb on - strandat 1213.476 kb on - strandat 1213.476 kb on - strandat 1213.477 kb on + strandat 1213.614 kb on + strandat 1213.618 kb on + strandat 1213.628 kb on + strandat 1213.629 kb on - strandat 1213.630 kb on + strandat 1213.640 kb on - strandat 1213.647 kb on - strandat 1213.648 kb on + strandat 1213.651 kb on + strandat 1213.651 kb on + strandat 1213.654 kb on + strandat 1213.708 kb on + strandat 1213.747 kb on - strandat 1213.748 kb on + strandat 1213.779 kb on - strand, within MMP_RS06300at 1213.833 kb on + strand, within MMP_RS06300at 1214.030 kb on - strand, within MMP_RS06300at 1214.030 kb on - strand, within MMP_RS06300at 1214.130 kb on + strand, within MMP_RS06300at 1214.130 kb on + strand, within MMP_RS06300at 1214.187 kb on + strand, within MMP_RS06300at 1214.217 kb on - strand, within MMP_RS06300at 1214.218 kb on + strand, within MMP_RS06300at 1214.220 kb on + strand, within MMP_RS06300at 1214.230 kb on - strand, within MMP_RS06300at 1214.242 kb on + strand, within MMP_RS06300at 1214.539 kb on + strandat 1214.539 kb on + strandat 1214.546 kb on + strandat 1214.678 kb on + strand, within MMP_RS06305at 1214.703 kb on - strand, within MMP_RS06305at 1214.704 kb on + strand, within MMP_RS06305at 1214.847 kb on + strand, within MMP_RS06305at 1215.060 kb on - strand, within MMP_RS06305at 1215.159 kb on + strand, within MMP_RS06305at 1215.175 kb on + strand, within MMP_RS06305at 1215.218 kb on - strand, within MMP_RS06305at 1215.258 kb on + strandat 1215.259 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine
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1,212,791 + MMP_RS06295 0.59 -3.6
1,212,947 + MMP_RS06295 0.70 -1.9
1,213,158 - MMP_RS06295 0.84 -3.1
1,213,174 - MMP_RS06295 0.85 -2.9
1,213,174 - MMP_RS06295 0.85 -5.4
1,213,394 - -3.0
1,213,408 + -1.6
1,213,413 - +0.1
1,213,476 - -0.1
1,213,476 - -0.0
1,213,477 + +0.3
1,213,614 + -0.0
1,213,618 + -0.3
1,213,628 + +0.5
1,213,629 - +0.2
1,213,630 + +0.2
1,213,640 - -0.0
1,213,647 - +0.5
1,213,648 + +0.9
1,213,651 + +0.2
1,213,651 + +0.2
1,213,654 + +0.5
1,213,708 + -0.8
1,213,747 - -0.2
1,213,748 + +0.5
1,213,779 - MMP_RS06300 0.13 +0.9
1,213,833 + MMP_RS06300 0.20 +0.3
1,214,030 - MMP_RS06300 0.45 +1.0
1,214,030 - MMP_RS06300 0.45 +0.8
1,214,130 + MMP_RS06300 0.59 +0.1
1,214,130 + MMP_RS06300 0.59 +0.5
1,214,187 + MMP_RS06300 0.66 -0.7
1,214,217 - MMP_RS06300 0.70 +0.7
1,214,218 + MMP_RS06300 0.70 +0.4
1,214,220 + MMP_RS06300 0.70 +0.8
1,214,230 - MMP_RS06300 0.72 +0.1
1,214,242 + MMP_RS06300 0.73 +1.7
1,214,539 + +0.8
1,214,539 + -0.3
1,214,546 + -0.5
1,214,678 + MMP_RS06305 0.15 +0.8
1,214,703 - MMP_RS06305 0.18 -0.5
1,214,704 + MMP_RS06305 0.19 -0.0
1,214,847 + MMP_RS06305 0.37 -0.1
1,215,060 - MMP_RS06305 0.65 +0.9
1,215,159 + MMP_RS06305 0.78 +0.5
1,215,175 + MMP_RS06305 0.80 +0.1
1,215,218 - MMP_RS06305 0.86 +0.8
1,215,258 + +0.2
1,215,259 - +0.9

Or see this region's nucleotide sequence