Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05820 and MMP_RS05825 are separated by 31 nucleotides MMP_RS05825 and MMP_RS05830 are separated by 41 nucleotides MMP_RS05830 and MMP_RS05835 are separated by 114 nucleotides
MMP_RS05820: MMP_RS05820 - RNA-binding transcriptional accessory protein, at 1,116,933 to 1,119,074
_RS05820
MMP_RS05825: MMP_RS05825 - peptidyl-prolyl cis-trans isomerase, at 1,119,106 to 1,119,717
_RS05825
MMP_RS05830: MMP_RS05830 - peptidyl-prolyl cis-trans isomerase, at 1,119,759 to 1,120,247
_RS05830
MMP_RS05835: MMP_RS05835 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,120,362 to 1,120,760
_RS05835
Position (kb)
1119
1120 Strain fitness (log2 ratio)
-1
0
1 at 1118.160 kb on - strand, within MMP_RS05820 at 1118.207 kb on + strand, within MMP_RS05820 at 1118.415 kb on - strand, within MMP_RS05820 at 1118.415 kb on - strand, within MMP_RS05820 at 1118.429 kb on - strand, within MMP_RS05820 at 1118.513 kb on - strand, within MMP_RS05820 at 1118.633 kb on - strand, within MMP_RS05820 at 1118.634 kb on + strand, within MMP_RS05820 at 1118.642 kb on + strand, within MMP_RS05820 at 1118.903 kb on - strand at 1118.963 kb on - strand at 1118.964 kb on + strand at 1118.983 kb on - strand at 1118.983 kb on - strand at 1119.095 kb on - strand at 1119.099 kb on - strand at 1119.100 kb on + strand at 1119.145 kb on - strand at 1119.145 kb on - strand at 1119.285 kb on + strand, within MMP_RS05825 at 1119.539 kb on + strand, within MMP_RS05825 at 1119.608 kb on + strand, within MMP_RS05825 at 1119.649 kb on + strand, within MMP_RS05825 at 1119.786 kb on - strand at 1119.787 kb on + strand at 1119.941 kb on - strand, within MMP_RS05830 at 1119.973 kb on + strand, within MMP_RS05830 at 1120.111 kb on + strand, within MMP_RS05830 at 1120.113 kb on - strand, within MMP_RS05830 at 1120.247 kb on - strand at 1120.566 kb on - strand, within MMP_RS05835
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,118,160 - MMP_RS05820 0.57 +1.3 1,118,207 + MMP_RS05820 0.59 +0.9 1,118,415 - MMP_RS05820 0.69 +0.4 1,118,415 - MMP_RS05820 0.69 +0.1 1,118,429 - MMP_RS05820 0.70 +0.4 1,118,513 - MMP_RS05820 0.74 -0.1 1,118,633 - MMP_RS05820 0.79 +0.3 1,118,634 + MMP_RS05820 0.79 +0.0 1,118,642 + MMP_RS05820 0.80 +0.1 1,118,903 - -0.9 1,118,963 - -0.1 1,118,964 + +0.8 1,118,983 - +0.3 1,118,983 - -0.0 1,119,095 - -0.9 1,119,099 - +0.7 1,119,100 + +0.6 1,119,145 - +0.8 1,119,145 - +0.7 1,119,285 + MMP_RS05825 0.29 +0.2 1,119,539 + MMP_RS05825 0.71 +0.5 1,119,608 + MMP_RS05825 0.82 +0.6 1,119,649 + MMP_RS05825 0.89 +0.3 1,119,786 - -0.1 1,119,787 + -0.4 1,119,941 - MMP_RS05830 0.37 -0.2 1,119,973 + MMP_RS05830 0.44 -0.6 1,120,111 + MMP_RS05830 0.72 +0.4 1,120,113 - MMP_RS05830 0.72 +1.3 1,120,247 - -1.3 1,120,566 - MMP_RS05835 0.51 +0.4
Or see this region's nucleotide sequence