Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05345 and MMP_RS05350 are separated by 21 nucleotides MMP_RS05350 and MMP_RS05355 are separated by 71 nucleotides MMP_RS05355 and MMP_RS05360 are separated by 37 nucleotides MMP_RS05360 and MMP_RS05365 are separated by 81 nucleotides
MMP_RS05345: MMP_RS05345 - hypothetical protein, at 1,025,552 to 1,028,482
_RS05345
MMP_RS05350: MMP_RS05350 - dTMP kinase, at 1,028,504 to 1,029,103
_RS05350
MMP_RS05355: MMP_RS05355 - hypothetical protein, at 1,029,175 to 1,029,588
_RS05355
MMP_RS05360: MMP_RS05360 - hypothetical protein, at 1,029,626 to 1,030,432
_RS05360
MMP_RS05365: MMP_RS05365 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,030,514 to 1,030,993
_RS05365
Position (kb)
1029
1030 Strain fitness (log2 ratio)
-2
-1
0
1 at 1028.176 kb on + strand, within MMP_RS05345 at 1028.330 kb on + strand at 1029.092 kb on + strand at 1029.096 kb on - strand at 1029.106 kb on - strand at 1029.107 kb on + strand at 1029.110 kb on - strand at 1029.110 kb on - strand at 1029.111 kb on + strand at 1029.111 kb on + strand at 1029.120 kb on + strand at 1029.127 kb on + strand at 1029.131 kb on - strand at 1029.143 kb on - strand at 1029.144 kb on + strand at 1029.144 kb on + strand at 1029.148 kb on + strand at 1029.148 kb on + strand at 1029.182 kb on - strand at 1029.183 kb on + strand at 1029.543 kb on + strand, within MMP_RS05355 at 1029.564 kb on + strand at 1029.564 kb on + strand at 1029.578 kb on - strand at 1029.625 kb on - strand at 1029.626 kb on + strand at 1029.626 kb on + strand at 1029.626 kb on + strand at 1029.649 kb on + strand at 1029.652 kb on + strand at 1029.655 kb on - strand at 1029.719 kb on + strand, within MMP_RS05360 at 1029.720 kb on - strand, within MMP_RS05360 at 1029.740 kb on + strand, within MMP_RS05360 at 1029.763 kb on - strand, within MMP_RS05360 at 1029.807 kb on - strand, within MMP_RS05360 at 1030.027 kb on - strand, within MMP_RS05360 at 1030.362 kb on - strand at 1030.363 kb on + strand at 1030.363 kb on + strand at 1030.396 kb on - strand at 1030.399 kb on + strand at 1030.514 kb on + strand at 1030.521 kb on - strand at 1030.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 1,028,176 + MMP_RS05345 0.90 -2.2 1,028,330 + -0.3 1,029,092 + -0.8 1,029,096 - -1.2 1,029,106 - +0.8 1,029,107 + -1.4 1,029,110 - +0.2 1,029,110 - +0.7 1,029,111 + +0.6 1,029,111 + +0.4 1,029,120 + -0.3 1,029,127 + +0.3 1,029,131 - +0.9 1,029,143 - +1.1 1,029,144 + -0.2 1,029,144 + +1.0 1,029,148 + -0.3 1,029,148 + +0.9 1,029,182 - +0.1 1,029,183 + +0.0 1,029,543 + MMP_RS05355 0.89 +0.5 1,029,564 + +0.3 1,029,564 + -0.8 1,029,578 - -0.3 1,029,625 - +1.6 1,029,626 + +0.6 1,029,626 + +0.3 1,029,626 + +0.4 1,029,649 + +0.6 1,029,652 + +0.7 1,029,655 - -1.1 1,029,719 + MMP_RS05360 0.12 -0.1 1,029,720 - MMP_RS05360 0.12 +1.0 1,029,740 + MMP_RS05360 0.14 -0.9 1,029,763 - MMP_RS05360 0.17 +0.1 1,029,807 - MMP_RS05360 0.22 -0.7 1,030,027 - MMP_RS05360 0.50 +0.0 1,030,362 - +0.1 1,030,363 + +0.4 1,030,363 + -1.2 1,030,396 - +0.7 1,030,399 + -0.3 1,030,514 + -0.2 1,030,521 - +1.3 1,030,522 + -1.4
Or see this region's nucleotide sequence