Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02900 and MMP_RS02905 are separated by 9 nucleotides MMP_RS02905 and MMP_RS02910 are separated by 170 nucleotides MMP_RS02910 and MMP_RS02915 are separated by 34 nucleotides MMP_RS02915 and MMP_RS02920 are separated by 77 nucleotides
MMP_RS02900: MMP_RS02900 - imidazoleglycerol-phosphate dehydratase HisB, at 552,862 to 553,434
_RS02900
MMP_RS02905: MMP_RS02905 - 2-thiouracil desulfurase family protein, at 553,444 to 553,992
_RS02905
MMP_RS02910: MMP_RS02910 - ABC transporter substrate-binding protein, at 554,163 to 554,957
_RS02910
MMP_RS02915: MMP_RS02915 - amino acid ABC transporter permease, at 554,992 to 555,651
_RS02915
MMP_RS02920: MMP_RS02920 - DMT family transporter, at 555,729 to 556,619
_RS02920
Position (kb) 554
555 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 553.313 kb on - strand, within MMP_RS02900 at 553.530 kb on - strand, within MMP_RS02905 at 553.717 kb on + strand, within MMP_RS02905 at 553.737 kb on + strand, within MMP_RS02905 at 553.746 kb on + strand, within MMP_RS02905 at 554.018 kb on - strand at 554.055 kb on - strand at 554.066 kb on - strand at 554.118 kb on - strand at 554.171 kb on - strand at 554.252 kb on - strand, within MMP_RS02910 at 554.294 kb on + strand, within MMP_RS02910 at 554.472 kb on - strand, within MMP_RS02910 at 554.678 kb on + strand, within MMP_RS02910 at 554.700 kb on - strand, within MMP_RS02910 at 554.766 kb on - strand, within MMP_RS02910 at 555.152 kb on - strand, within MMP_RS02915 at 555.164 kb on - strand, within MMP_RS02915 at 555.211 kb on - strand, within MMP_RS02915 at 555.423 kb on - strand, within MMP_RS02915 at 555.683 kb on - strand at 555.684 kb on + strand at 555.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 553,313 - MMP_RS02900 0.79 -3.8 553,530 - MMP_RS02905 0.16 -0.4 553,717 + MMP_RS02905 0.50 +0.8 553,737 + MMP_RS02905 0.53 -0.5 553,746 + MMP_RS02905 0.55 -0.0 554,018 - -1.8 554,055 - -0.8 554,066 - -0.6 554,118 - -0.4 554,171 - -1.8 554,252 - MMP_RS02910 0.11 -0.3 554,294 + MMP_RS02910 0.16 +0.1 554,472 - MMP_RS02910 0.39 +0.7 554,678 + MMP_RS02910 0.65 -0.0 554,700 - MMP_RS02910 0.68 +1.1 554,766 - MMP_RS02910 0.76 +0.8 555,152 - MMP_RS02915 0.24 -0.1 555,164 - MMP_RS02915 0.26 -0.4 555,211 - MMP_RS02915 0.33 +0.0 555,423 - MMP_RS02915 0.65 +0.0 555,683 - -0.3 555,684 + +0.9 555,699 - +0.2
Or see this region's nucleotide sequence