Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02795 and MMP_RS02800 are separated by 230 nucleotides MMP_RS02800 and MMP_RS02805 overlap by 14 nucleotides MMP_RS02805 and MMP_RS02810 are separated by 157 nucleotides
MMP_RS02795: MMP_RS02795 - sulfate permease, at 533,633 to 535,291
_RS02795
MMP_RS02800: MMP_RS02800 - nucleotidyltransferase domain-containing protein, at 535,522 to 536,139
_RS02800
MMP_RS02805: MMP_RS02805 - HEPN domain-containing protein, at 536,126 to 536,635
_RS02805
MMP_RS02810: MMP_RS02810 - hypothetical protein, at 536,793 to 538,262
_RS02810
Position (kb) 536
537 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 535.244 kb on - strand at 535.272 kb on + strand at 535.292 kb on + strand at 535.293 kb on - strand at 535.294 kb on + strand at 535.294 kb on + strand at 535.330 kb on - strand at 535.356 kb on - strand at 535.377 kb on - strand at 535.379 kb on - strand at 535.381 kb on - strand at 535.381 kb on - strand at 535.382 kb on + strand at 535.382 kb on + strand at 535.382 kb on + strand at 535.382 kb on + strand at 535.385 kb on - strand at 535.421 kb on - strand at 535.422 kb on + strand at 535.424 kb on + strand at 535.424 kb on + strand at 535.427 kb on - strand at 535.428 kb on + strand at 535.428 kb on + strand at 535.436 kb on + strand at 535.496 kb on - strand at 535.496 kb on - strand at 535.496 kb on - strand at 535.496 kb on - strand at 535.497 kb on + strand at 535.497 kb on + strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.499 kb on + strand at 535.499 kb on + strand at 535.499 kb on + strand at 535.499 kb on + strand at 535.502 kb on - strand at 535.509 kb on + strand at 535.541 kb on - strand at 535.541 kb on - strand at 535.542 kb on + strand at 535.542 kb on + strand at 535.543 kb on - strand at 535.552 kb on - strand at 535.553 kb on + strand at 535.572 kb on - strand at 535.577 kb on - strand at 535.693 kb on - strand, within MMP_RS02800 at 535.693 kb on - strand, within MMP_RS02800 at 535.696 kb on - strand, within MMP_RS02800 at 535.696 kb on - strand, within MMP_RS02800 at 535.697 kb on + strand, within MMP_RS02800 at 535.697 kb on + strand, within MMP_RS02800 at 535.697 kb on + strand, within MMP_RS02800 at 535.998 kb on + strand, within MMP_RS02800 at 536.013 kb on + strand, within MMP_RS02800 at 536.060 kb on - strand, within MMP_RS02800 at 536.144 kb on - strand at 536.318 kb on + strand, within MMP_RS02805 at 536.714 kb on - strand at 536.742 kb on - strand at 536.761 kb on - strand at 536.761 kb on - strand at 536.761 kb on - strand at 536.773 kb on + strand at 536.848 kb on - strand at 536.850 kb on - strand at 536.851 kb on + strand at 537.199 kb on - strand, within MMP_RS02810 at 537.239 kb on + strand, within MMP_RS02810 at 537.242 kb on + strand, within MMP_RS02810 at 537.243 kb on - strand, within MMP_RS02810 at 537.272 kb on - strand, within MMP_RS02810 at 537.273 kb on + strand, within MMP_RS02810 at 537.275 kb on + strand, within MMP_RS02810 at 537.277 kb on + strand, within MMP_RS02810 at 537.277 kb on + strand, within MMP_RS02810 at 537.314 kb on + strand, within MMP_RS02810 at 537.406 kb on - strand, within MMP_RS02810 at 537.450 kb on - strand, within MMP_RS02810 at 537.450 kb on - strand, within MMP_RS02810
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 535,244 - +0.8 535,272 + +0.4 535,292 + +0.5 535,293 - +1.7 535,294 + -0.1 535,294 + -0.1 535,330 - +0.9 535,356 - +0.5 535,377 - -0.4 535,379 - +0.5 535,381 - +0.2 535,381 - +0.6 535,382 + +0.9 535,382 + +0.0 535,382 + +0.2 535,382 + +0.2 535,385 - +0.9 535,421 - +0.3 535,422 + +0.0 535,424 + -0.3 535,424 + +0.5 535,427 - +0.0 535,428 + +0.3 535,428 + -0.3 535,436 + +0.5 535,496 - -2.3 535,496 - -1.1 535,496 - -1.0 535,496 - -1.8 535,497 + -1.2 535,497 + -1.5 535,498 - -2.6 535,498 - -3.1 535,498 - -3.7 535,498 - -2.4 535,498 - -4.4 535,498 - -1.5 535,498 - -2.9 535,499 + -1.9 535,499 + -2.0 535,499 + -3.2 535,499 + -5.7 535,502 - -2.5 535,509 + -2.5 535,541 - -3.5 535,541 - -3.4 535,542 + -3.3 535,542 + -1.9 535,543 - -2.5 535,552 - -3.0 535,553 + -2.9 535,572 - -2.2 535,577 - -1.8 535,693 - MMP_RS02800 0.28 -3.1 535,693 - MMP_RS02800 0.28 -2.6 535,696 - MMP_RS02800 0.28 -3.5 535,696 - MMP_RS02800 0.28 -1.5 535,697 + MMP_RS02800 0.28 -3.0 535,697 + MMP_RS02800 0.28 -2.8 535,697 + MMP_RS02800 0.28 -2.5 535,998 + MMP_RS02800 0.77 -2.6 536,013 + MMP_RS02800 0.79 -3.2 536,060 - MMP_RS02800 0.87 -0.5 536,144 - +0.7 536,318 + MMP_RS02805 0.38 +0.6 536,714 - +1.5 536,742 - -0.3 536,761 - +0.6 536,761 - +0.8 536,761 - +0.5 536,773 + -0.4 536,848 - +0.4 536,850 - +1.3 536,851 + +0.6 537,199 - MMP_RS02810 0.28 +1.5 537,239 + MMP_RS02810 0.30 -0.3 537,242 + MMP_RS02810 0.31 -0.5 537,243 - MMP_RS02810 0.31 +0.4 537,272 - MMP_RS02810 0.33 +0.7 537,273 + MMP_RS02810 0.33 +0.1 537,275 + MMP_RS02810 0.33 +0.9 537,277 + MMP_RS02810 0.33 +0.1 537,277 + MMP_RS02810 0.33 +1.6 537,314 + MMP_RS02810 0.35 +0.8 537,406 - MMP_RS02810 0.42 +0.5 537,450 - MMP_RS02810 0.45 +0.4 537,450 - MMP_RS02810 0.45 +0.3
Or see this region's nucleotide sequence