Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02730 and MMP_RS02735 are separated by 27 nucleotides MMP_RS02735 and MMP_RS02740 are separated by 80 nucleotides MMP_RS02740 and MMP_RS02745 are separated by 96 nucleotides MMP_RS02745 and MMP_RS02750 are separated by 15 nucleotides
MMP_RS02730: MMP_RS02730 - ModD protein, at 521,710 to 522,561
_RS02730
MMP_RS02735: MMP_RS02735 - MBL fold metallo-hydrolase, at 522,589 to 523,119
_RS02735
MMP_RS02740: MMP_RS02740 - hypothetical protein, at 523,200 to 523,469
_RS02740
MMP_RS02745: MMP_RS02745 - hypothetical protein, at 523,566 to 523,943
_RS02745
MMP_RS02750: MMP_RS02750 - HAD-IC family P-type ATPase, at 523,959 to 526,463
_RS02750
Position (kb) 523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 522.461 kb on - strand, within MMP_RS02730 at 522.469 kb on - strand, within MMP_RS02730 at 522.542 kb on - strand at 522.563 kb on - strand at 522.583 kb on - strand at 522.586 kb on + strand at 522.719 kb on + strand, within MMP_RS02735 at 522.759 kb on + strand, within MMP_RS02735 at 522.759 kb on + strand, within MMP_RS02735 at 522.811 kb on + strand, within MMP_RS02735 at 522.833 kb on + strand, within MMP_RS02735 at 522.922 kb on - strand, within MMP_RS02735 at 522.931 kb on - strand, within MMP_RS02735 at 523.013 kb on + strand, within MMP_RS02735 at 523.174 kb on + strand at 523.186 kb on - strand at 523.191 kb on - strand at 523.299 kb on - strand, within MMP_RS02740 at 523.368 kb on - strand, within MMP_RS02740 at 523.397 kb on - strand, within MMP_RS02740 at 523.414 kb on + strand, within MMP_RS02740 at 523.515 kb on - strand at 523.515 kb on - strand at 523.517 kb on - strand at 523.520 kb on - strand at 523.521 kb on + strand at 523.523 kb on + strand at 523.525 kb on - strand at 523.629 kb on + strand, within MMP_RS02745 at 523.840 kb on + strand, within MMP_RS02745 at 524.212 kb on - strand, within MMP_RS02750 at 524.212 kb on - strand, within MMP_RS02750 at 524.214 kb on - strand, within MMP_RS02750 at 524.289 kb on - strand, within MMP_RS02750 at 524.289 kb on - strand, within MMP_RS02750 at 524.289 kb on - strand, within MMP_RS02750 at 524.290 kb on + strand, within MMP_RS02750 at 524.290 kb on + strand, within MMP_RS02750 at 524.292 kb on - strand, within MMP_RS02750 at 524.292 kb on - strand, within MMP_RS02750 at 524.293 kb on + strand, within MMP_RS02750 at 524.296 kb on - strand, within MMP_RS02750 at 524.302 kb on - strand, within MMP_RS02750 at 524.364 kb on + strand, within MMP_RS02750 at 524.366 kb on + strand, within MMP_RS02750 at 524.410 kb on + strand, within MMP_RS02750 at 524.466 kb on + strand, within MMP_RS02750
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 522,461 - MMP_RS02730 0.88 -1.6 522,469 - MMP_RS02730 0.89 -3.7 522,542 - -2.8 522,563 - +0.4 522,583 - -0.8 522,586 + +1.1 522,719 + MMP_RS02735 0.24 +0.0 522,759 + MMP_RS02735 0.32 +0.6 522,759 + MMP_RS02735 0.32 +0.5 522,811 + MMP_RS02735 0.42 -0.0 522,833 + MMP_RS02735 0.46 -0.3 522,922 - MMP_RS02735 0.63 -1.1 522,931 - MMP_RS02735 0.64 +1.1 523,013 + MMP_RS02735 0.80 -0.6 523,174 + -0.4 523,186 - +0.7 523,191 - +0.9 523,299 - MMP_RS02740 0.37 +1.0 523,368 - MMP_RS02740 0.62 +0.6 523,397 - MMP_RS02740 0.73 +0.3 523,414 + MMP_RS02740 0.79 +0.6 523,515 - -0.3 523,515 - -0.1 523,517 - +0.4 523,520 - -0.1 523,521 + +0.7 523,523 + -0.1 523,525 - +0.1 523,629 + MMP_RS02745 0.17 -0.3 523,840 + MMP_RS02745 0.72 -0.3 524,212 - MMP_RS02750 0.10 -0.0 524,212 - MMP_RS02750 0.10 +0.9 524,214 - MMP_RS02750 0.10 -0.9 524,289 - MMP_RS02750 0.13 -0.8 524,289 - MMP_RS02750 0.13 -2.2 524,289 - MMP_RS02750 0.13 +0.7 524,290 + MMP_RS02750 0.13 +0.9 524,290 + MMP_RS02750 0.13 -0.3 524,292 - MMP_RS02750 0.13 -0.4 524,292 - MMP_RS02750 0.13 +0.3 524,293 + MMP_RS02750 0.13 +0.2 524,296 - MMP_RS02750 0.13 +0.1 524,302 - MMP_RS02750 0.14 +0.2 524,364 + MMP_RS02750 0.16 -0.2 524,366 + MMP_RS02750 0.16 -0.2 524,410 + MMP_RS02750 0.18 +1.4 524,466 + MMP_RS02750 0.20 +0.2
Or see this region's nucleotide sequence