Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02290 and MMP_RS02295 are separated by 170 nucleotides MMP_RS02295 and MMP_RS02300 are separated by 18 nucleotides MMP_RS02300 and MMP_RS02305 are separated by 38 nucleotides MMP_RS02305 and MMP_RS02310 are separated by 266 nucleotides
MMP_RS02290: MMP_RS02290 - MBL fold metallo-hydrolase, at 431,730 to 433,031
_RS02290
MMP_RS02295: MMP_RS02295 - TatD family hydrolase, at 433,202 to 433,963
_RS02295
MMP_RS02300: MMP_RS02300 - NAD(P)H-dependent oxidoreductase, at 433,982 to 434,476
_RS02300
MMP_RS02305: MMP_RS02305 - DUF378 domain-containing protein, at 434,515 to 434,763
_RS02305
MMP_RS02310: MMP_RS02310 - RimK-like ATPgrasp N-terminal domain-containing protein, at 435,030 to 435,635
_RS02310
Position (kb) 433
434
435 Strain fitness (log2 ratio) -2
-1
0
1 at 433.063 kb on - strand at 433.064 kb on + strand at 433.077 kb on - strand at 433.079 kb on - strand at 433.090 kb on + strand at 433.102 kb on + strand at 433.131 kb on - strand at 433.403 kb on + strand, within MMP_RS02295 at 433.635 kb on - strand, within MMP_RS02295 at 433.677 kb on - strand, within MMP_RS02295 at 433.707 kb on - strand, within MMP_RS02295 at 433.800 kb on + strand, within MMP_RS02295 at 433.825 kb on - strand, within MMP_RS02295 at 433.826 kb on + strand, within MMP_RS02295 at 433.831 kb on - strand, within MMP_RS02295 at 433.831 kb on - strand, within MMP_RS02295 at 433.832 kb on + strand, within MMP_RS02295 at 433.852 kb on - strand, within MMP_RS02295 at 433.852 kb on - strand, within MMP_RS02295 at 433.853 kb on + strand, within MMP_RS02295 at 433.854 kb on - strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.930 kb on - strand at 433.933 kb on - strand at 433.933 kb on - strand at 433.947 kb on - strand at 433.961 kb on - strand at 433.979 kb on - strand at 433.981 kb on - strand at 434.007 kb on + strand at 434.007 kb on + strand at 434.009 kb on - strand at 434.033 kb on - strand, within MMP_RS02300 at 434.199 kb on + strand, within MMP_RS02300 at 434.207 kb on + strand, within MMP_RS02300 at 434.208 kb on - strand, within MMP_RS02300 at 434.332 kb on - strand, within MMP_RS02300 at 434.449 kb on - strand at 434.449 kb on - strand at 434.456 kb on - strand at 434.457 kb on + strand at 434.457 kb on + strand at 434.474 kb on - strand at 434.532 kb on + strand at 434.535 kb on + strand at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.543 kb on - strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.544 kb on + strand, within MMP_RS02305 at 434.572 kb on - strand, within MMP_RS02305 at 434.653 kb on + strand, within MMP_RS02305 at 434.665 kb on - strand, within MMP_RS02305 at 434.679 kb on - strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.680 kb on + strand, within MMP_RS02305 at 434.728 kb on + strand, within MMP_RS02305 at 434.803 kb on - strand at 434.803 kb on - strand at 435.312 kb on - strand, within MMP_RS02310
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 433,063 - +1.7 433,064 + +1.5 433,077 - +0.6 433,079 - +1.2 433,090 + -1.7 433,102 + +0.3 433,131 - +0.9 433,403 + MMP_RS02295 0.26 +0.3 433,635 - MMP_RS02295 0.57 -0.9 433,677 - MMP_RS02295 0.62 +0.5 433,707 - MMP_RS02295 0.66 -0.2 433,800 + MMP_RS02295 0.78 -0.4 433,825 - MMP_RS02295 0.82 +0.8 433,826 + MMP_RS02295 0.82 +0.3 433,831 - MMP_RS02295 0.83 -0.4 433,831 - MMP_RS02295 0.83 +0.4 433,832 + MMP_RS02295 0.83 -0.2 433,852 - MMP_RS02295 0.85 -0.6 433,852 - MMP_RS02295 0.85 +0.0 433,853 + MMP_RS02295 0.85 -0.7 433,854 - MMP_RS02295 0.86 -0.1 433,855 + MMP_RS02295 0.86 +1.0 433,855 + MMP_RS02295 0.86 +0.6 433,855 + MMP_RS02295 0.86 -0.3 433,855 + MMP_RS02295 0.86 +0.2 433,855 + MMP_RS02295 0.86 +0.2 433,930 - +1.2 433,933 - -0.2 433,933 - -0.5 433,947 - +0.2 433,961 - +0.8 433,979 - -2.1 433,981 - +0.5 434,007 + -1.1 434,007 + +0.1 434,009 - +1.1 434,033 - MMP_RS02300 0.10 +0.9 434,199 + MMP_RS02300 0.44 +0.2 434,207 + MMP_RS02300 0.45 +0.6 434,208 - MMP_RS02300 0.46 +1.0 434,332 - MMP_RS02300 0.71 +0.5 434,449 - +0.2 434,449 - -0.0 434,456 - +0.9 434,457 + -1.2 434,457 + -0.1 434,474 - +0.9 434,532 + +0.1 434,535 + +0.2 434,543 - MMP_RS02305 0.11 -0.3 434,543 - MMP_RS02305 0.11 +0.7 434,543 - MMP_RS02305 0.11 +0.1 434,543 - MMP_RS02305 0.11 +0.7 434,543 - MMP_RS02305 0.11 -1.4 434,543 - MMP_RS02305 0.11 +0.6 434,543 - MMP_RS02305 0.11 +0.6 434,543 - MMP_RS02305 0.11 -0.1 434,543 - MMP_RS02305 0.11 +0.9 434,544 + MMP_RS02305 0.12 -0.3 434,544 + MMP_RS02305 0.12 +0.5 434,544 + MMP_RS02305 0.12 -0.4 434,572 - MMP_RS02305 0.23 +0.4 434,653 + MMP_RS02305 0.55 -0.8 434,665 - MMP_RS02305 0.60 -0.3 434,679 - MMP_RS02305 0.66 -0.1 434,680 + MMP_RS02305 0.66 +1.1 434,680 + MMP_RS02305 0.66 +0.7 434,728 + MMP_RS02305 0.86 +0.2 434,803 - +0.5 434,803 - +0.1 435,312 - MMP_RS02310 0.47 +0.5
Or see this region's nucleotide sequence