Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02280 and MMP_RS02285 are separated by 54 nucleotides MMP_RS02285 and MMP_RS02290 are separated by 62 nucleotides MMP_RS02290 and MMP_RS02295 are separated by 170 nucleotides MMP_RS02295 and MMP_RS02300 are separated by 18 nucleotides
MMP_RS02280: MMP_RS02280 - DUF211 domain-containing protein, at 430,621 to 430,914
_RS02280
MMP_RS02285: MMP_RS02285 - RNase P subunit p30 family protein, at 430,969 to 431,667
_RS02285
MMP_RS02290: MMP_RS02290 - MBL fold metallo-hydrolase, at 431,730 to 433,031
_RS02290
MMP_RS02295: MMP_RS02295 - TatD family hydrolase, at 433,202 to 433,963
_RS02295
MMP_RS02300: MMP_RS02300 - NAD(P)H-dependent oxidoreductase, at 433,982 to 434,476
_RS02300
Position (kb) 431
432
433
434 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 430.819 kb on + strand, within MMP_RS02280 at 430.906 kb on + strand at 430.906 kb on + strand at 430.906 kb on + strand at 430.909 kb on - strand at 430.938 kb on - strand at 430.941 kb on - strand at 431.138 kb on + strand at 431.391 kb on + strand at 431.409 kb on - strand at 431.500 kb on + strand at 431.504 kb on + strand at 431.755 kb on + strand at 431.832 kb on - strand at 431.947 kb on + strand, within MMP_RS02290 at 432.147 kb on + strand, within MMP_RS02290 at 432.167 kb on - strand, within MMP_RS02290 at 432.190 kb on + strand, within MMP_RS02290 at 432.242 kb on + strand, within MMP_RS02290 at 432.286 kb on - strand, within MMP_RS02290 at 432.318 kb on - strand, within MMP_RS02290 at 432.454 kb on - strand, within MMP_RS02290 at 432.959 kb on - strand at 433.063 kb on - strand at 433.064 kb on + strand at 433.077 kb on - strand at 433.079 kb on - strand at 433.090 kb on + strand at 433.102 kb on + strand at 433.131 kb on - strand at 433.403 kb on + strand, within MMP_RS02295 at 433.635 kb on - strand, within MMP_RS02295 at 433.677 kb on - strand, within MMP_RS02295 at 433.707 kb on - strand, within MMP_RS02295 at 433.800 kb on + strand, within MMP_RS02295 at 433.825 kb on - strand, within MMP_RS02295 at 433.826 kb on + strand, within MMP_RS02295 at 433.831 kb on - strand, within MMP_RS02295 at 433.831 kb on - strand, within MMP_RS02295 at 433.832 kb on + strand, within MMP_RS02295 at 433.852 kb on - strand, within MMP_RS02295 at 433.852 kb on - strand, within MMP_RS02295 at 433.853 kb on + strand, within MMP_RS02295 at 433.854 kb on - strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.855 kb on + strand, within MMP_RS02295 at 433.930 kb on - strand at 433.933 kb on - strand at 433.933 kb on - strand at 433.947 kb on - strand at 433.961 kb on - strand at 433.979 kb on - strand at 433.981 kb on - strand at 434.007 kb on + strand at 434.007 kb on + strand at 434.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 430,819 + MMP_RS02280 0.67 -0.5 430,906 + -0.2 430,906 + -0.6 430,906 + -2.5 430,909 - -1.4 430,938 - -1.2 430,941 - -1.6 431,138 + -3.2 431,391 + -0.6 431,409 - -3.1 431,500 + -0.9 431,504 + -2.7 431,755 + +0.6 431,832 - +0.5 431,947 + MMP_RS02290 0.17 +1.5 432,147 + MMP_RS02290 0.32 +1.6 432,167 - MMP_RS02290 0.34 +1.8 432,190 + MMP_RS02290 0.35 +1.6 432,242 + MMP_RS02290 0.39 +1.2 432,286 - MMP_RS02290 0.43 +2.3 432,318 - MMP_RS02290 0.45 +1.4 432,454 - MMP_RS02290 0.56 +1.4 432,959 - +0.9 433,063 - +1.7 433,064 + +1.5 433,077 - +0.6 433,079 - +1.2 433,090 + -1.7 433,102 + +0.3 433,131 - +0.9 433,403 + MMP_RS02295 0.26 +0.3 433,635 - MMP_RS02295 0.57 -0.9 433,677 - MMP_RS02295 0.62 +0.5 433,707 - MMP_RS02295 0.66 -0.2 433,800 + MMP_RS02295 0.78 -0.4 433,825 - MMP_RS02295 0.82 +0.8 433,826 + MMP_RS02295 0.82 +0.3 433,831 - MMP_RS02295 0.83 -0.4 433,831 - MMP_RS02295 0.83 +0.4 433,832 + MMP_RS02295 0.83 -0.2 433,852 - MMP_RS02295 0.85 -0.6 433,852 - MMP_RS02295 0.85 +0.0 433,853 + MMP_RS02295 0.85 -0.7 433,854 - MMP_RS02295 0.86 -0.1 433,855 + MMP_RS02295 0.86 +1.0 433,855 + MMP_RS02295 0.86 +0.6 433,855 + MMP_RS02295 0.86 -0.3 433,855 + MMP_RS02295 0.86 +0.2 433,855 + MMP_RS02295 0.86 +0.2 433,930 - +1.2 433,933 - -0.2 433,933 - -0.5 433,947 - +0.2 433,961 - +0.8 433,979 - -2.1 433,981 - +0.5 434,007 + -1.1 434,007 + +0.1 434,009 - +1.1
Or see this region's nucleotide sequence