Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02040 and MMP_RS02045 are separated by 213 nucleotides MMP_RS02045 and MMP_RS02050 are separated by 86 nucleotides MMP_RS02050 and MMP_RS02055 are separated by 114 nucleotides MMP_RS02055 and MMP_RS02060 are separated by 105 nucleotides
MMP_RS02040: MMP_RS02040 - S-layer protein, at 381,781 to 383,508
_RS02040
MMP_RS02045: MMP_RS02045 - tRNA-Met, at 383,722 to 383,800
_RS02045
MMP_RS02050: MMP_RS02050 - tRNA-Met, at 383,887 to 383,965
_RS02050
MMP_RS02055: MMP_RS02055 - DUF2116 family Zn-ribbon domain-containing protein, at 384,080 to 384,262
_RS02055
MMP_RS02060: MMP_RS02060 - UMP kinase, at 384,368 to 385,045
_RS02060
Position (kb)
383
384 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 383.511 kb on + strand at 383.622 kb on - strand at 383.623 kb on + strand at 383.690 kb on - strand at 383.695 kb on + strand at 383.707 kb on - strand at 383.792 kb on - strand, within MMP_RS02045 at 383.822 kb on - strand at 384.007 kb on - strand at 384.096 kb on - strand at 384.097 kb on + strand at 384.097 kb on + strand at 384.306 kb on - strand at 384.307 kb on + strand at 384.310 kb on - strand at 384.354 kb on + strand at 384.740 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 383,511 + -0.9 383,622 - +0.8 383,623 + +0.8 383,690 - -0.5 383,695 + -0.7 383,707 - +0.3 383,792 - MMP_RS02045 0.89 +1.1 383,822 - -0.1 384,007 - -2.9 384,096 - +0.5 384,097 + +0.9 384,097 + +0.3 384,306 - -0.6 384,307 + -0.4 384,310 - -0.1 384,354 + +0.9 384,740 + -0.4
Or see this region's nucleotide sequence