Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01645 and MMP_RS01650 are separated by 156 nucleotides MMP_RS01650 and MMP_RS01655 are separated by 9 nucleotides MMP_RS01655 and MMP_RS01660 are separated by 124 nucleotides MMP_RS01660 and MMP_RS01665 are separated by 54 nucleotides MMP_RS01665 and MMP_RS01670 are separated by 20 nucleotides MMP_RS01670 and MMP_RS01675 are separated by 8 nucleotides
MMP_RS01645: MMP_RS01645 - SWIM zinc finger family protein, at 305,126 to 305,815
_RS01645
MMP_RS01650: MMP_RS01650 - sulfurtransferase TusA family protein, at 305,972 to 306,184
_RS01650
MMP_RS01655: MMP_RS01655 - DsrE family protein, at 306,194 to 306,538
_RS01655
MMP_RS01660: MMP_RS01660 - hypothetical protein, at 306,663 to 307,247
_RS01660
MMP_RS01665: MMP_RS01665 - DUF2111 domain-containing protein, at 307,302 to 307,685
_RS01665
MMP_RS01670: MMP_RS01670 - methanogenesis marker 5 protein, at 307,706 to 308,146
_RS01670
MMP_RS01675: MMP_RS01675 - hypothetical protein, at 308,155 to 308,610
_RS01675
Position (kb) 306
307
308 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 305.773 kb on - strand at 305.792 kb on - strand at 305.792 kb on - strand at 305.884 kb on + strand at 306.118 kb on - strand, within MMP_RS01650 at 306.169 kb on + strand at 306.174 kb on - strand at 306.210 kb on - strand at 306.323 kb on - strand, within MMP_RS01655 at 306.365 kb on - strand, within MMP_RS01655 at 306.499 kb on + strand, within MMP_RS01655 at 306.521 kb on + strand at 306.545 kb on - strand at 306.558 kb on - strand at 306.590 kb on + strand at 306.603 kb on - strand at 306.617 kb on - strand at 306.622 kb on - strand at 306.636 kb on - strand at 306.636 kb on - strand at 306.641 kb on - strand at 306.647 kb on + strand at 306.663 kb on + strand at 306.663 kb on + strand at 306.744 kb on - strand, within MMP_RS01660 at 306.745 kb on + strand, within MMP_RS01660 at 306.756 kb on + strand, within MMP_RS01660 at 306.999 kb on + strand, within MMP_RS01660 at 307.249 kb on - strand at 307.263 kb on + strand at 307.272 kb on - strand at 307.272 kb on - strand at 307.273 kb on + strand at 307.273 kb on + strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.277 kb on + strand at 307.277 kb on + strand at 307.277 kb on + strand at 307.288 kb on - strand at 307.288 kb on - strand at 307.289 kb on + strand at 307.337 kb on + strand at 307.351 kb on + strand, within MMP_RS01665 at 307.355 kb on - strand, within MMP_RS01665 at 307.363 kb on - strand, within MMP_RS01665 at 307.363 kb on - strand, within MMP_RS01665 at 307.450 kb on + strand, within MMP_RS01665 at 307.609 kb on - strand, within MMP_RS01665 at 307.684 kb on + strand at 308.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 305,773 - -0.8 305,792 - -0.5 305,792 - +0.2 305,884 + -0.5 306,118 - MMP_RS01650 0.69 +1.1 306,169 + -0.1 306,174 - +0.8 306,210 - +1.6 306,323 - MMP_RS01655 0.37 -0.3 306,365 - MMP_RS01655 0.50 -1.1 306,499 + MMP_RS01655 0.88 +0.1 306,521 + +1.1 306,545 - +0.7 306,558 - +0.9 306,590 + +0.6 306,603 - +1.1 306,617 - +1.2 306,622 - -0.8 306,636 - +0.3 306,636 - -0.4 306,641 - +0.6 306,647 + -1.2 306,663 + -0.4 306,663 + +0.4 306,744 - MMP_RS01660 0.14 +1.1 306,745 + MMP_RS01660 0.14 -0.6 306,756 + MMP_RS01660 0.16 +0.4 306,999 + MMP_RS01660 0.57 +1.0 307,249 - -0.4 307,263 + -0.3 307,272 - -1.3 307,272 - -1.7 307,273 + +0.2 307,273 + +0.7 307,276 - -2.5 307,276 - -3.6 307,276 - -2.9 307,276 - -2.8 307,276 - -3.9 307,276 - -2.2 307,277 + -1.0 307,277 + +0.3 307,277 + -1.4 307,288 - -2.2 307,288 - -1.6 307,289 + -0.1 307,337 + +0.6 307,351 + MMP_RS01665 0.13 -0.4 307,355 - MMP_RS01665 0.14 -3.4 307,363 - MMP_RS01665 0.16 -0.9 307,363 - MMP_RS01665 0.16 -2.1 307,450 + MMP_RS01665 0.39 -0.1 307,609 - MMP_RS01665 0.80 -0.8 307,684 + +0.4 308,172 - -0.1
Or see this region's nucleotide sequence