Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01270 and MMP_RS01275 are separated by 1 nucleotides MMP_RS01275 and MMP_RS01280 are separated by 125 nucleotides MMP_RS01280 and MMP_RS01285 overlap by 1 nucleotides MMP_RS01285 and MMP_RS01290 are separated by 33 nucleotides
MMP_RS01270: MMP_RS01270 - prepilin peptidase, at 239,930 to 240,970
_RS01270
MMP_RS01275: MMP_RS01275 - class III signal peptide-containing protein, at 240,972 to 241,361
_RS01275
MMP_RS01280: MMP_RS01280 - DUF515 domain-containing protein, at 241,487 to 242,635
_RS01280
MMP_RS01285: MMP_RS01285 - hypothetical protein, at 242,635 to 243,528
_RS01285
MMP_RS01290: MMP_RS01290 - class III signal peptide-containing protein, at 243,562 to 243,960
_RS01290
Position (kb)
241
242
243 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 240.510 kb on + strand, within MMP_RS01270 at 240.514 kb on + strand, within MMP_RS01270 at 240.566 kb on - strand, within MMP_RS01270 at 240.601 kb on + strand, within MMP_RS01270 at 240.666 kb on - strand, within MMP_RS01270 at 240.683 kb on + strand, within MMP_RS01270 at 240.688 kb on + strand, within MMP_RS01270 at 240.705 kb on - strand, within MMP_RS01270 at 240.724 kb on - strand, within MMP_RS01270 at 240.884 kb on + strand at 240.884 kb on + strand at 240.934 kb on + strand at 240.969 kb on + strand at 241.047 kb on + strand, within MMP_RS01275 at 241.221 kb on + strand, within MMP_RS01275 at 241.232 kb on - strand, within MMP_RS01275 at 241.235 kb on + strand, within MMP_RS01275 at 241.243 kb on + strand, within MMP_RS01275 at 241.244 kb on - strand, within MMP_RS01275 at 241.383 kb on + strand at 241.400 kb on - strand at 241.407 kb on - strand at 241.408 kb on + strand at 241.414 kb on + strand at 241.429 kb on - strand at 241.429 kb on - strand at 241.429 kb on - strand at 241.430 kb on + strand at 241.430 kb on + strand at 241.431 kb on - strand at 241.435 kb on - strand at 241.451 kb on - strand at 241.465 kb on + strand at 241.486 kb on - strand at 241.502 kb on - strand at 241.502 kb on - strand at 241.511 kb on + strand at 241.691 kb on + strand, within MMP_RS01280 at 241.742 kb on - strand, within MMP_RS01280 at 241.743 kb on + strand, within MMP_RS01280 at 241.743 kb on + strand, within MMP_RS01280 at 241.755 kb on - strand, within MMP_RS01280 at 241.822 kb on + strand, within MMP_RS01280 at 241.829 kb on - strand, within MMP_RS01280 at 241.829 kb on - strand, within MMP_RS01280 at 241.832 kb on + strand, within MMP_RS01280 at 241.862 kb on + strand, within MMP_RS01280 at 241.862 kb on + strand, within MMP_RS01280 at 241.866 kb on + strand, within MMP_RS01280 at 241.874 kb on - strand, within MMP_RS01280 at 242.036 kb on - strand, within MMP_RS01280 at 242.042 kb on - strand, within MMP_RS01280 at 242.043 kb on + strand, within MMP_RS01280 at 242.043 kb on + strand, within MMP_RS01280 at 242.067 kb on + strand, within MMP_RS01280 at 242.067 kb on + strand, within MMP_RS01280 at 242.152 kb on - strand, within MMP_RS01280 at 242.230 kb on + strand, within MMP_RS01280 at 242.309 kb on - strand, within MMP_RS01280 at 242.390 kb on - strand, within MMP_RS01280 at 242.446 kb on - strand, within MMP_RS01280 at 242.495 kb on + strand, within MMP_RS01280 at 242.518 kb on + strand, within MMP_RS01280 at 242.547 kb on - strand at 242.547 kb on - strand at 242.548 kb on + strand at 242.606 kb on - strand at 242.630 kb on + strand at 242.652 kb on + strand at 242.656 kb on - strand at 242.656 kb on - strand at 242.721 kb on - strand at 242.722 kb on + strand at 242.734 kb on - strand, within MMP_RS01285 at 242.758 kb on + strand, within MMP_RS01285 at 242.892 kb on - strand, within MMP_RS01285 at 243.008 kb on - strand, within MMP_RS01285 at 243.009 kb on + strand, within MMP_RS01285 at 243.011 kb on - strand, within MMP_RS01285 at 243.015 kb on - strand, within MMP_RS01285 at 243.072 kb on + strand, within MMP_RS01285 at 243.114 kb on + strand, within MMP_RS01285 at 243.114 kb on + strand, within MMP_RS01285 at 243.188 kb on - strand, within MMP_RS01285 at 243.239 kb on - strand, within MMP_RS01285 at 243.292 kb on + strand, within MMP_RS01285 at 243.292 kb on + strand, within MMP_RS01285 at 243.292 kb on + strand, within MMP_RS01285 at 243.393 kb on + strand, within MMP_RS01285 at 243.437 kb on + strand, within MMP_RS01285 at 243.439 kb on + strand at 243.442 kb on + strand at 243.532 kb on + strand at 243.558 kb on - strand at 243.629 kb on - strand, within MMP_RS01290
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 240,510 + MMP_RS01270 0.56 +0.9 240,514 + MMP_RS01270 0.56 +0.2 240,566 - MMP_RS01270 0.61 -2.9 240,601 + MMP_RS01270 0.64 +0.3 240,666 - MMP_RS01270 0.71 -0.7 240,683 + MMP_RS01270 0.72 +0.5 240,688 + MMP_RS01270 0.73 +0.5 240,705 - MMP_RS01270 0.74 -0.3 240,724 - MMP_RS01270 0.76 +1.3 240,884 + -0.3 240,884 + +0.1 240,934 + -0.4 240,969 + +1.0 241,047 + MMP_RS01275 0.19 +0.4 241,221 + MMP_RS01275 0.64 -0.0 241,232 - MMP_RS01275 0.67 +0.7 241,235 + MMP_RS01275 0.67 +0.1 241,243 + MMP_RS01275 0.69 +0.3 241,244 - MMP_RS01275 0.70 +0.8 241,383 + +0.7 241,400 - +0.5 241,407 - +1.0 241,408 + +1.1 241,414 + +0.8 241,429 - +0.1 241,429 - +0.8 241,429 - +0.9 241,430 + +1.0 241,430 + -0.7 241,431 - +0.3 241,435 - -0.0 241,451 - +0.5 241,465 + +0.6 241,486 - +0.0 241,502 - +0.9 241,502 - -0.3 241,511 + +1.7 241,691 + MMP_RS01280 0.18 +0.4 241,742 - MMP_RS01280 0.22 +1.4 241,743 + MMP_RS01280 0.22 +0.7 241,743 + MMP_RS01280 0.22 +1.2 241,755 - MMP_RS01280 0.23 +1.1 241,822 + MMP_RS01280 0.29 -0.3 241,829 - MMP_RS01280 0.30 +0.7 241,829 - MMP_RS01280 0.30 -0.7 241,832 + MMP_RS01280 0.30 +0.4 241,862 + MMP_RS01280 0.33 +0.6 241,862 + MMP_RS01280 0.33 +1.3 241,866 + MMP_RS01280 0.33 +0.1 241,874 - MMP_RS01280 0.34 -0.1 242,036 - MMP_RS01280 0.48 +0.4 242,042 - MMP_RS01280 0.48 +0.2 242,043 + MMP_RS01280 0.48 -0.0 242,043 + MMP_RS01280 0.48 +0.4 242,067 + MMP_RS01280 0.50 +1.2 242,067 + MMP_RS01280 0.50 +1.5 242,152 - MMP_RS01280 0.58 +0.4 242,230 + MMP_RS01280 0.65 +1.0 242,309 - MMP_RS01280 0.72 +0.2 242,390 - MMP_RS01280 0.79 +1.1 242,446 - MMP_RS01280 0.83 +0.6 242,495 + MMP_RS01280 0.88 +0.2 242,518 + MMP_RS01280 0.90 +0.0 242,547 - +0.7 242,547 - +0.1 242,548 + +0.9 242,606 - +0.6 242,630 + -0.5 242,652 + +1.0 242,656 - -0.9 242,656 - +0.6 242,721 - +0.1 242,722 + -0.5 242,734 - MMP_RS01285 0.11 +0.8 242,758 + MMP_RS01285 0.14 +0.3 242,892 - MMP_RS01285 0.29 -0.9 243,008 - MMP_RS01285 0.42 -0.5 243,009 + MMP_RS01285 0.42 -0.8 243,011 - MMP_RS01285 0.42 +0.1 243,015 - MMP_RS01285 0.43 +0.6 243,072 + MMP_RS01285 0.49 -0.1 243,114 + MMP_RS01285 0.54 +0.3 243,114 + MMP_RS01285 0.54 -0.3 243,188 - MMP_RS01285 0.62 -0.0 243,239 - MMP_RS01285 0.68 +0.0 243,292 + MMP_RS01285 0.73 +0.0 243,292 + MMP_RS01285 0.73 +0.3 243,292 + MMP_RS01285 0.73 +1.0 243,393 + MMP_RS01285 0.85 -0.2 243,437 + MMP_RS01285 0.90 +0.7 243,439 + +1.3 243,442 + -1.0 243,532 + +0.6 243,558 - -0.2 243,629 - MMP_RS01290 0.17 +1.0
Or see this region's nucleotide sequence