Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01130 and MMP_RS01135 are separated by 526 nucleotides MMP_RS01135 and MMP_RS01140 are separated by 9 nucleotides MMP_RS01140 and MMP_RS01145 are separated by 9 nucleotides
MMP_RS01130: MMP_RS01130 - flavoprotein, at 211,471 to 212,001
_RS01130
MMP_RS01135: MMP_RS01135 - molybdate ABC transporter substrate-binding protein, at 212,528 to 213,331
_RS01135
MMP_RS01140: MMP_RS01140 - molybdate ABC transporter permease subunit, at 213,341 to 214,006
_RS01140
MMP_RS01145: MMP_RS01145 - ABC transporter ATP-binding protein, at 214,016 to 215,158
_RS01145
Position (kb) 212
213
214 Strain fitness (log2 ratio) -1
0
1
2 at 211.678 kb on - strand, within MMP_RS01130 at 211.678 kb on - strand, within MMP_RS01130 at 211.746 kb on - strand, within MMP_RS01130 at 211.747 kb on + strand, within MMP_RS01130 at 211.770 kb on - strand, within MMP_RS01130 at 211.773 kb on + strand, within MMP_RS01130 at 211.806 kb on - strand, within MMP_RS01130 at 211.807 kb on + strand, within MMP_RS01130 at 211.880 kb on - strand, within MMP_RS01130 at 212.008 kb on - strand at 212.008 kb on - strand at 212.009 kb on + strand at 212.012 kb on - strand at 212.013 kb on + strand at 212.017 kb on + strand at 212.018 kb on - strand at 212.129 kb on - strand at 212.129 kb on - strand at 212.129 kb on - strand at 212.177 kb on - strand at 212.281 kb on - strand at 212.286 kb on - strand at 212.454 kb on - strand at 212.454 kb on - strand at 212.454 kb on - strand at 212.457 kb on + strand at 212.457 kb on + strand at 212.512 kb on + strand at 212.512 kb on + strand at 212.512 kb on + strand at 212.514 kb on + strand at 212.551 kb on + strand at 212.812 kb on - strand, within MMP_RS01135 at 212.823 kb on - strand, within MMP_RS01135 at 212.830 kb on + strand, within MMP_RS01135 at 212.830 kb on + strand, within MMP_RS01135 at 212.830 kb on + strand, within MMP_RS01135 at 212.926 kb on - strand, within MMP_RS01135 at 212.927 kb on + strand, within MMP_RS01135 at 212.927 kb on + strand, within MMP_RS01135 at 212.944 kb on + strand, within MMP_RS01135 at 212.977 kb on - strand, within MMP_RS01135 at 213.129 kb on - strand, within MMP_RS01135 at 213.132 kb on + strand, within MMP_RS01135 at 213.132 kb on + strand, within MMP_RS01135 at 213.132 kb on + strand, within MMP_RS01135 at 213.175 kb on - strand, within MMP_RS01135 at 213.176 kb on + strand, within MMP_RS01135 at 213.176 kb on + strand, within MMP_RS01135 at 213.177 kb on - strand, within MMP_RS01135 at 213.177 kb on - strand, within MMP_RS01135 at 213.178 kb on + strand, within MMP_RS01135 at 213.206 kb on + strand, within MMP_RS01135 at 213.206 kb on + strand, within MMP_RS01135 at 213.217 kb on + strand, within MMP_RS01135 at 213.217 kb on + strand, within MMP_RS01135 at 213.220 kb on - strand, within MMP_RS01135 at 213.220 kb on - strand, within MMP_RS01135 at 213.269 kb on - strand at 213.270 kb on + strand at 213.336 kb on + strand at 213.354 kb on - strand at 213.355 kb on + strand at 213.355 kb on + strand at 213.366 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.388 kb on - strand at 213.390 kb on - strand at 213.395 kb on + strand at 213.395 kb on + strand at 213.395 kb on + strand at 213.480 kb on - strand, within MMP_RS01140 at 213.480 kb on - strand, within MMP_RS01140 at 213.597 kb on - strand, within MMP_RS01140 at 213.934 kb on + strand, within MMP_RS01140 at 213.949 kb on + strand at 214.004 kb on - strand at 214.016 kb on + strand at 214.114 kb on + strand at 214.262 kb on + strand, within MMP_RS01145 at 214.305 kb on + strand, within MMP_RS01145
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 211,678 - MMP_RS01130 0.39 +0.3 211,678 - MMP_RS01130 0.39 +0.2 211,746 - MMP_RS01130 0.52 +0.5 211,747 + MMP_RS01130 0.52 -0.5 211,770 - MMP_RS01130 0.56 +0.1 211,773 + MMP_RS01130 0.57 +0.4 211,806 - MMP_RS01130 0.63 +1.2 211,807 + MMP_RS01130 0.63 +0.4 211,880 - MMP_RS01130 0.77 +0.4 212,008 - +0.2 212,008 - -0.5 212,009 + -0.4 212,012 - +0.4 212,013 + +1.8 212,017 + +1.0 212,018 - +0.2 212,129 - -0.1 212,129 - -0.4 212,129 - +0.1 212,177 - -0.3 212,281 - +1.3 212,286 - +0.2 212,454 - +1.0 212,454 - -0.6 212,454 - -0.5 212,457 + +1.1 212,457 + +2.1 212,512 + -0.5 212,512 + +1.2 212,512 + +0.2 212,514 + -0.2 212,551 + -1.1 212,812 - MMP_RS01135 0.35 +0.9 212,823 - MMP_RS01135 0.37 +0.2 212,830 + MMP_RS01135 0.38 +0.9 212,830 + MMP_RS01135 0.38 +2.1 212,830 + MMP_RS01135 0.38 +0.5 212,926 - MMP_RS01135 0.50 +0.3 212,927 + MMP_RS01135 0.50 +0.5 212,927 + MMP_RS01135 0.50 +1.1 212,944 + MMP_RS01135 0.52 +0.9 212,977 - MMP_RS01135 0.56 +0.5 213,129 - MMP_RS01135 0.75 +1.6 213,132 + MMP_RS01135 0.75 +0.0 213,132 + MMP_RS01135 0.75 +1.3 213,132 + MMP_RS01135 0.75 +1.4 213,175 - MMP_RS01135 0.80 -0.2 213,176 + MMP_RS01135 0.81 +0.1 213,176 + MMP_RS01135 0.81 -0.1 213,177 - MMP_RS01135 0.81 +0.3 213,177 - MMP_RS01135 0.81 +1.1 213,178 + MMP_RS01135 0.81 +1.6 213,206 + MMP_RS01135 0.84 +1.1 213,206 + MMP_RS01135 0.84 +0.7 213,217 + MMP_RS01135 0.86 +0.7 213,217 + MMP_RS01135 0.86 +0.6 213,220 - MMP_RS01135 0.86 +1.3 213,220 - MMP_RS01135 0.86 +1.0 213,269 - +0.3 213,270 + +1.1 213,336 + +0.5 213,354 - -1.0 213,355 + +0.7 213,355 + +0.2 213,366 - +0.9 213,370 - -0.2 213,370 - -0.3 213,370 - +0.1 213,370 - -1.2 213,370 - +1.0 213,388 - -0.5 213,390 - +0.6 213,395 + +1.0 213,395 + -0.3 213,395 + -0.0 213,480 - MMP_RS01140 0.21 +0.1 213,480 - MMP_RS01140 0.21 +0.4 213,597 - MMP_RS01140 0.38 -1.7 213,934 + MMP_RS01140 0.89 +0.3 213,949 + -0.0 214,004 - +1.6 214,016 + +0.7 214,114 + +0.4 214,262 + MMP_RS01145 0.22 -0.3 214,305 + MMP_RS01145 0.25 +0.6
Or see this region's nucleotide sequence