Experiment: H2 N-free with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00985 and MMP_RS00990 are separated by 112 nucleotides MMP_RS00990 and MMP_RS00995 are separated by 135 nucleotides MMP_RS00995 and MMP_RS01000 are separated by 9 nucleotides
MMP_RS00985: MMP_RS00985 - CDC48 family AAA ATPase, at 183,704 to 186,070
_RS00985
MMP_RS00990: MMP_RS00990 - hypothetical protein, at 186,183 to 186,542
_RS00990
MMP_RS00995: MMP_RS00995 - phosphoribosylformylglycinamidine synthase I, at 186,678 to 187,487
_RS00995
MMP_RS01000: MMP_RS01000 - AIR synthase related protein, at 187,497 to 190,466
_RS01000
Position (kb) 186
187 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 185.544 kb on - strand, within MMP_RS00985 at 185.545 kb on + strand, within MMP_RS00985 at 185.711 kb on - strand, within MMP_RS00985 at 185.885 kb on - strand at 186.029 kb on - strand at 186.174 kb on - strand at 186.415 kb on - strand, within MMP_RS00990 at 186.505 kb on - strand, within MMP_RS00990 at 186.560 kb on - strand at 186.574 kb on + strand at 186.625 kb on + strand at 186.683 kb on - strand at 187.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Glycine remove 185,544 - MMP_RS00985 0.78 -2.5 185,545 + MMP_RS00985 0.78 -3.8 185,711 - MMP_RS00985 0.85 -1.4 185,885 - +0.1 186,029 - -2.4 186,174 - +0.0 186,415 - MMP_RS00990 0.64 +1.3 186,505 - MMP_RS00990 0.89 +0.2 186,560 - +0.3 186,574 + +0.6 186,625 + -0.0 186,683 - -2.5 187,170 - -1.2
Or see this region's nucleotide sequence