Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09530 and PGA1_c09540 are separated by 27 nucleotides PGA1_c09540 and PGA1_c09550 are separated by 331 nucleotides
PGA1_c09530: PGA1_c09530 - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase MurF, at 977,137 to 978,549
_c09530
PGA1_c09540: PGA1_c09540 - phospho-N-acetylmuramoyl-pentapeptide- transferase MraY, at 978,577 to 979,659
_c09540
PGA1_c09550: PGA1_c09550 - Glutaminase (EC 3.5.1.2) <small>(from data)</small>, at 979,991 to 980,905
_c09550
Position (kb) 978
979
980 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 979.653 kb on + strand at 979.840 kb on + strand at 979.840 kb on + strand at 979.924 kb on + strand at 979.924 kb on + strand at 979.932 kb on - strand at 979.937 kb on + strand at 979.938 kb on - strand at 979.938 kb on - strand at 980.066 kb on + strand at 980.074 kb on - strand at 980.113 kb on + strand, within PGA1_c09550 at 980.206 kb on - strand, within PGA1_c09550 at 980.238 kb on - strand, within PGA1_c09550 at 980.291 kb on - strand, within PGA1_c09550 at 980.358 kb on + strand, within PGA1_c09550 at 980.358 kb on + strand, within PGA1_c09550 at 980.400 kb on + strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.487 kb on - strand, within PGA1_c09550 at 980.515 kb on + strand, within PGA1_c09550 at 980.515 kb on + strand, within PGA1_c09550 at 980.520 kb on + strand, within PGA1_c09550 at 980.536 kb on + strand, within PGA1_c09550 at 980.544 kb on + strand, within PGA1_c09550 at 980.556 kb on - strand, within PGA1_c09550 at 980.623 kb on - strand, within PGA1_c09550 at 980.635 kb on - strand, within PGA1_c09550
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 979,653 + -0.3 979,840 + +1.0 979,840 + +1.5 979,924 + -2.8 979,924 + -2.1 979,932 - +1.5 979,937 + +2.7 979,938 - +1.6 979,938 - +1.0 980,066 + +1.7 980,074 - +1.5 980,113 + PGA1_c09550 0.13 -1.0 980,206 - PGA1_c09550 0.23 +3.0 980,238 - PGA1_c09550 0.27 -2.6 980,291 - PGA1_c09550 0.33 +1.2 980,358 + PGA1_c09550 0.40 -2.3 980,358 + PGA1_c09550 0.40 -1.6 980,400 + PGA1_c09550 0.45 -0.1 980,408 - PGA1_c09550 0.46 +1.4 980,408 - PGA1_c09550 0.46 -1.8 980,408 - PGA1_c09550 0.46 +1.5 980,408 - PGA1_c09550 0.46 -0.9 980,487 - PGA1_c09550 0.54 +2.7 980,515 + PGA1_c09550 0.57 -2.3 980,515 + PGA1_c09550 0.57 -0.8 980,520 + PGA1_c09550 0.58 -1.6 980,536 + PGA1_c09550 0.60 -2.3 980,544 + PGA1_c09550 0.60 -2.1 980,556 - PGA1_c09550 0.62 +2.1 980,623 - PGA1_c09550 0.69 +1.1 980,635 - PGA1_c09550 0.70 -1.9
Or see this region's nucleotide sequence