Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00930 and PGA1_c00940 overlap by 8 nucleotides PGA1_c00940 and PGA1_c00950 overlap by 4 nucleotides PGA1_c00950 and PGA1_c00960 overlap by 4 nucleotides PGA1_c00960 and PGA1_c00970 are separated by 121 nucleotides
PGA1_c00930: PGA1_c00930 - ribonuclease H, at 89,612 to 90,079
_c00930
PGA1_c00940: PGA1_c00940 - putative methyltransferase, at 90,072 to 90,671
_c00940
PGA1_c00950: PGA1_c00950 - Protein of unknown function (DUF3429)., at 90,668 to 91,120
_c00950
PGA1_c00960: PGA1_c00960 - lysE type translocator-like protein, at 91,117 to 91,734
_c00960
PGA1_c00970: PGA1_c00970 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase IspH, at 91,856 to 92,806
_c00970
Position (kb) 90
91
92 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 89.826 kb on - strand, within PGA1_c00930 at 90.097 kb on - strand at 90.112 kb on - strand at 90.136 kb on + strand, within PGA1_c00940 at 90.142 kb on + strand, within PGA1_c00940 at 90.144 kb on - strand, within PGA1_c00940 at 90.205 kb on + strand, within PGA1_c00940 at 90.205 kb on + strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.215 kb on + strand, within PGA1_c00940 at 90.215 kb on + strand, within PGA1_c00940 at 90.215 kb on + strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.240 kb on - strand, within PGA1_c00940 at 90.249 kb on + strand, within PGA1_c00940 at 90.259 kb on + strand, within PGA1_c00940 at 90.259 kb on + strand, within PGA1_c00940 at 90.287 kb on - strand, within PGA1_c00940 at 90.351 kb on + strand, within PGA1_c00940 at 90.367 kb on - strand, within PGA1_c00940 at 90.397 kb on - strand, within PGA1_c00940 at 90.408 kb on + strand, within PGA1_c00940 at 90.473 kb on + strand, within PGA1_c00940 at 90.497 kb on - strand, within PGA1_c00940 at 90.499 kb on + strand, within PGA1_c00940 at 90.507 kb on - strand, within PGA1_c00940 at 90.530 kb on - strand, within PGA1_c00940 at 90.559 kb on - strand, within PGA1_c00940 at 90.601 kb on + strand, within PGA1_c00940 at 90.674 kb on - strand at 90.677 kb on - strand at 90.681 kb on + strand at 90.686 kb on + strand at 90.689 kb on - strand at 90.689 kb on - strand at 90.711 kb on - strand at 90.738 kb on - strand, within PGA1_c00950 at 90.773 kb on + strand, within PGA1_c00950 at 90.773 kb on + strand, within PGA1_c00950 at 90.781 kb on - strand, within PGA1_c00950 at 90.824 kb on + strand, within PGA1_c00950 at 90.824 kb on + strand, within PGA1_c00950 at 90.824 kb on + strand, within PGA1_c00950 at 90.824 kb on + strand, within PGA1_c00950 at 90.832 kb on - strand, within PGA1_c00950 at 90.878 kb on + strand, within PGA1_c00950 at 90.878 kb on + strand, within PGA1_c00950 at 90.879 kb on + strand, within PGA1_c00950 at 90.963 kb on + strand, within PGA1_c00950 at 90.971 kb on - strand, within PGA1_c00950 at 91.025 kb on - strand, within PGA1_c00950 at 91.025 kb on - strand, within PGA1_c00950 at 91.032 kb on + strand, within PGA1_c00950 at 91.040 kb on - strand, within PGA1_c00950 at 91.051 kb on + strand, within PGA1_c00950 at 91.056 kb on + strand, within PGA1_c00950 at 91.077 kb on - strand at 91.077 kb on - strand at 91.079 kb on + strand at 91.123 kb on - strand at 91.238 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.264 kb on + strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.272 kb on - strand, within PGA1_c00960 at 91.284 kb on + strand, within PGA1_c00960 at 91.368 kb on + strand, within PGA1_c00960 at 91.368 kb on + strand, within PGA1_c00960 at 91.402 kb on - strand, within PGA1_c00960 at 91.523 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.536 kb on + strand, within PGA1_c00960 at 91.538 kb on + strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.544 kb on - strand, within PGA1_c00960 at 91.551 kb on - strand, within PGA1_c00960 at 91.551 kb on - strand, within PGA1_c00960 at 91.554 kb on - strand, within PGA1_c00960 at 91.554 kb on - strand, within PGA1_c00960 at 91.632 kb on - strand, within PGA1_c00960 at 91.639 kb on + strand, within PGA1_c00960 at 91.644 kb on + strand, within PGA1_c00960 at 91.644 kb on + strand, within PGA1_c00960 at 91.656 kb on + strand, within PGA1_c00960 at 91.664 kb on + strand, within PGA1_c00960 at 91.664 kb on + strand, within PGA1_c00960 at 91.674 kb on + strand at 91.674 kb on + strand at 91.674 kb on + strand at 91.674 kb on + strand at 91.677 kb on - strand at 91.693 kb on + strand at 91.719 kb on - strand at 91.758 kb on + strand at 91.758 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.768 kb on + strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.776 kb on - strand at 91.794 kb on + strand at 91.820 kb on + strand at 91.830 kb on - strand at 91.830 kb on - strand at 91.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 89,826 - PGA1_c00930 0.46 +0.2 90,097 - +1.2 90,112 - -1.4 90,136 + PGA1_c00940 0.11 +1.4 90,142 + PGA1_c00940 0.12 -2.1 90,144 - PGA1_c00940 0.12 +1.7 90,205 + PGA1_c00940 0.22 +1.4 90,205 + PGA1_c00940 0.22 -2.3 90,213 - PGA1_c00940 0.23 +1.8 90,213 - PGA1_c00940 0.23 -1.3 90,213 - PGA1_c00940 0.23 +1.4 90,213 - PGA1_c00940 0.23 -1.8 90,213 - PGA1_c00940 0.23 -3.8 90,213 - PGA1_c00940 0.23 -0.1 90,213 - PGA1_c00940 0.23 +0.7 90,213 - PGA1_c00940 0.23 +2.8 90,215 + PGA1_c00940 0.24 +0.0 90,215 + PGA1_c00940 0.24 +1.5 90,215 + PGA1_c00940 0.24 +2.2 90,223 - PGA1_c00940 0.25 +1.9 90,223 - PGA1_c00940 0.25 +3.7 90,223 - PGA1_c00940 0.25 -2.8 90,223 - PGA1_c00940 0.25 +3.2 90,223 - PGA1_c00940 0.25 -2.2 90,240 - PGA1_c00940 0.28 +2.0 90,249 + PGA1_c00940 0.29 +2.8 90,259 + PGA1_c00940 0.31 -2.5 90,259 + PGA1_c00940 0.31 +2.6 90,287 - PGA1_c00940 0.36 -2.1 90,351 + PGA1_c00940 0.47 -2.9 90,367 - PGA1_c00940 0.49 -2.7 90,397 - PGA1_c00940 0.54 -2.7 90,408 + PGA1_c00940 0.56 -2.3 90,473 + PGA1_c00940 0.67 +2.8 90,497 - PGA1_c00940 0.71 +0.7 90,499 + PGA1_c00940 0.71 -1.3 90,507 - PGA1_c00940 0.72 -0.4 90,530 - PGA1_c00940 0.76 -1.4 90,559 - PGA1_c00940 0.81 -1.6 90,601 + PGA1_c00940 0.88 -0.8 90,674 - -2.4 90,677 - -0.7 90,681 + +2.3 90,686 + +1.9 90,689 - +1.1 90,689 - -0.0 90,711 - +0.9 90,738 - PGA1_c00950 0.15 +2.2 90,773 + PGA1_c00950 0.23 +2.9 90,773 + PGA1_c00950 0.23 +0.5 90,781 - PGA1_c00950 0.25 +2.3 90,824 + PGA1_c00950 0.34 -2.3 90,824 + PGA1_c00950 0.34 -2.2 90,824 + PGA1_c00950 0.34 +2.7 90,824 + PGA1_c00950 0.34 -1.2 90,832 - PGA1_c00950 0.36 +0.3 90,878 + PGA1_c00950 0.46 +1.8 90,878 + PGA1_c00950 0.46 -2.2 90,879 + PGA1_c00950 0.47 +1.9 90,963 + PGA1_c00950 0.65 -1.3 90,971 - PGA1_c00950 0.67 -1.4 91,025 - PGA1_c00950 0.79 -1.6 91,025 - PGA1_c00950 0.79 +1.2 91,032 + PGA1_c00950 0.80 +3.4 91,040 - PGA1_c00950 0.82 +1.2 91,051 + PGA1_c00950 0.85 +2.7 91,056 + PGA1_c00950 0.86 -1.1 91,077 - +2.6 91,077 - +3.5 91,079 + +2.9 91,123 - +1.4 91,238 + PGA1_c00960 0.20 +1.2 91,264 + PGA1_c00960 0.24 -2.9 91,264 + PGA1_c00960 0.24 -0.2 91,264 + PGA1_c00960 0.24 -2.8 91,264 + PGA1_c00960 0.24 -2.6 91,264 + PGA1_c00960 0.24 +0.4 91,272 - PGA1_c00960 0.25 -0.7 91,272 - PGA1_c00960 0.25 -2.2 91,272 - PGA1_c00960 0.25 +0.8 91,272 - PGA1_c00960 0.25 -0.7 91,272 - PGA1_c00960 0.25 -2.4 91,272 - PGA1_c00960 0.25 -1.2 91,272 - PGA1_c00960 0.25 +1.9 91,272 - PGA1_c00960 0.25 -2.6 91,272 - PGA1_c00960 0.25 -0.8 91,272 - PGA1_c00960 0.25 +2.7 91,284 + PGA1_c00960 0.27 -1.0 91,368 + PGA1_c00960 0.41 -2.2 91,368 + PGA1_c00960 0.41 -0.8 91,402 - PGA1_c00960 0.46 +0.8 91,523 + PGA1_c00960 0.66 +0.2 91,536 + PGA1_c00960 0.68 +2.5 91,536 + PGA1_c00960 0.68 -2.2 91,536 + PGA1_c00960 0.68 -0.4 91,536 + PGA1_c00960 0.68 +2.5 91,536 + PGA1_c00960 0.68 -1.5 91,536 + PGA1_c00960 0.68 +1.9 91,536 + PGA1_c00960 0.68 +1.5 91,536 + PGA1_c00960 0.68 -1.9 91,536 + PGA1_c00960 0.68 -2.8 91,538 + PGA1_c00960 0.68 +1.8 91,544 - PGA1_c00960 0.69 -3.2 91,544 - PGA1_c00960 0.69 +1.1 91,544 - PGA1_c00960 0.69 +2.6 91,544 - PGA1_c00960 0.69 -0.4 91,544 - PGA1_c00960 0.69 -1.1 91,551 - PGA1_c00960 0.70 -0.6 91,551 - PGA1_c00960 0.70 +2.8 91,554 - PGA1_c00960 0.71 -2.0 91,554 - PGA1_c00960 0.71 -2.4 91,632 - PGA1_c00960 0.83 -1.7 91,639 + PGA1_c00960 0.84 +1.3 91,644 + PGA1_c00960 0.85 -1.2 91,644 + PGA1_c00960 0.85 +0.9 91,656 + PGA1_c00960 0.87 -0.8 91,664 + PGA1_c00960 0.89 -2.3 91,664 + PGA1_c00960 0.89 -1.2 91,674 + -1.7 91,674 + +0.7 91,674 + +0.5 91,674 + +1.6 91,677 - -0.9 91,693 + -2.3 91,719 - +3.9 91,758 + -2.2 91,758 + -0.6 91,768 + +1.2 91,768 + +1.5 91,768 + -1.4 91,768 + +2.9 91,768 + +1.1 91,768 + -0.7 91,768 + -1.2 91,768 + +1.4 91,776 - +2.5 91,776 - -2.4 91,776 - +1.8 91,776 - +1.4 91,776 - -1.9 91,776 - -1.5 91,776 - -2.5 91,776 - -0.3 91,776 - -1.1 91,776 - -1.8 91,776 - +1.6 91,776 - -1.5 91,776 - +3.8 91,776 - -1.0 91,776 - -1.2 91,794 + -1.4 91,820 + -2.0 91,830 - +1.6 91,830 - -2.2 91,847 - +1.6
Or see this region's nucleotide sequence