Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c08520 and PGA1_c08530 overlap by 4 nucleotides PGA1_c08530 and PGA1_c08540 are separated by 259 nucleotides PGA1_c08540 and PGA1_c08550 are separated by 115 nucleotides PGA1_c08550 and PGA1_c08560 are separated by 238 nucleotides
PGA1_c08520: PGA1_c08520 - hypothetical protein, at 879,321 to 879,797
_c08520
PGA1_c08530: PGA1_c08530 - Predicted integral membrane protein, at 879,794 to 880,273
_c08530
PGA1_c08540: PGA1_c08540 - response regulator PleD, at 880,533 to 881,963
_c08540
PGA1_c08550: PGA1_c08550 - Protein of unknown function (DUF3572)., at 882,079 to 882,363
_c08550
PGA1_c08560: PGA1_c08560 - hydrolase, HAD-superfamily, at 882,602 to 883,291
_c08560
Position (kb) 880
881
882 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 879.560 kb on + strand, within PGA1_c08520 at 880.473 kb on - strand at 880.632 kb on + strand at 880.678 kb on + strand, within PGA1_c08540 at 880.678 kb on + strand, within PGA1_c08540 at 880.800 kb on + strand, within PGA1_c08540 at 880.841 kb on - strand, within PGA1_c08540 at 880.903 kb on + strand, within PGA1_c08540 at 880.903 kb on + strand, within PGA1_c08540 at 880.936 kb on + strand, within PGA1_c08540 at 880.944 kb on - strand, within PGA1_c08540 at 880.953 kb on - strand, within PGA1_c08540 at 881.006 kb on + strand, within PGA1_c08540 at 881.025 kb on - strand, within PGA1_c08540 at 881.110 kb on + strand, within PGA1_c08540 at 881.145 kb on - strand, within PGA1_c08540 at 881.201 kb on - strand, within PGA1_c08540 at 881.382 kb on + strand, within PGA1_c08540 at 881.396 kb on - strand, within PGA1_c08540 at 881.398 kb on + strand, within PGA1_c08540 at 881.490 kb on + strand, within PGA1_c08540 at 881.505 kb on + strand, within PGA1_c08540 at 881.513 kb on - strand, within PGA1_c08540 at 881.513 kb on - strand, within PGA1_c08540 at 881.518 kb on - strand, within PGA1_c08540 at 881.530 kb on - strand, within PGA1_c08540 at 881.543 kb on + strand, within PGA1_c08540 at 881.543 kb on + strand, within PGA1_c08540 at 881.546 kb on - strand, within PGA1_c08540 at 881.546 kb on - strand, within PGA1_c08540 at 881.814 kb on - strand, within PGA1_c08540 at 881.832 kb on + strand at 881.871 kb on - strand at 881.900 kb on + strand at 881.908 kb on - strand at 881.908 kb on - strand at 881.925 kb on + strand at 881.933 kb on - strand at 881.948 kb on + strand at 881.948 kb on + strand at 881.955 kb on + strand at 881.956 kb on - strand at 881.964 kb on - strand at 881.967 kb on + strand at 881.971 kb on - strand at 881.971 kb on - strand at 881.972 kb on + strand at 881.972 kb on + strand at 881.979 kb on - strand at 881.980 kb on - strand at 882.016 kb on + strand at 882.066 kb on - strand at 882.113 kb on - strand, within PGA1_c08550 at 882.179 kb on + strand, within PGA1_c08550 at 882.188 kb on + strand, within PGA1_c08550 at 882.348 kb on + strand at 882.391 kb on + strand at 882.398 kb on + strand at 882.408 kb on + strand at 882.472 kb on - strand at 882.525 kb on + strand at 882.539 kb on + strand at 882.560 kb on - strand at 882.567 kb on + strand at 882.567 kb on + strand at 882.575 kb on - strand at 882.575 kb on - strand at 882.575 kb on - strand at 882.592 kb on + strand at 882.593 kb on + strand at 882.606 kb on - strand at 882.639 kb on - strand at 882.668 kb on + strand at 882.692 kb on - strand, within PGA1_c08560 at 882.849 kb on - strand, within PGA1_c08560 at 882.898 kb on + strand, within PGA1_c08560 at 882.898 kb on + strand, within PGA1_c08560 at 882.946 kb on + strand, within PGA1_c08560 at 882.952 kb on + strand, within PGA1_c08560 at 882.960 kb on + strand, within PGA1_c08560 at 882.960 kb on - strand, within PGA1_c08560
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 879,560 + PGA1_c08520 0.50 -1.4 880,473 - +0.7 880,632 + -1.2 880,678 + PGA1_c08540 0.10 -0.4 880,678 + PGA1_c08540 0.10 -1.5 880,800 + PGA1_c08540 0.19 +0.9 880,841 - PGA1_c08540 0.22 -2.0 880,903 + PGA1_c08540 0.26 -2.4 880,903 + PGA1_c08540 0.26 -2.9 880,936 + PGA1_c08540 0.28 -0.1 880,944 - PGA1_c08540 0.29 -2.9 880,953 - PGA1_c08540 0.29 +2.6 881,006 + PGA1_c08540 0.33 -2.0 881,025 - PGA1_c08540 0.34 -3.5 881,110 + PGA1_c08540 0.40 +1.3 881,145 - PGA1_c08540 0.43 -2.3 881,201 - PGA1_c08540 0.47 -1.3 881,382 + PGA1_c08540 0.59 -1.7 881,396 - PGA1_c08540 0.60 -0.5 881,398 + PGA1_c08540 0.60 +2.3 881,490 + PGA1_c08540 0.67 +0.3 881,505 + PGA1_c08540 0.68 -1.2 881,513 - PGA1_c08540 0.68 +2.2 881,513 - PGA1_c08540 0.68 +2.7 881,518 - PGA1_c08540 0.69 +2.9 881,530 - PGA1_c08540 0.70 +0.7 881,543 + PGA1_c08540 0.71 +3.9 881,543 + PGA1_c08540 0.71 -0.6 881,546 - PGA1_c08540 0.71 +1.0 881,546 - PGA1_c08540 0.71 -2.5 881,814 - PGA1_c08540 0.90 -2.1 881,832 + +0.7 881,871 - -1.1 881,900 + +2.4 881,908 - +2.5 881,908 - -0.6 881,925 + -2.6 881,933 - +2.3 881,948 + -2.1 881,948 + +0.7 881,955 + +1.6 881,956 - -3.1 881,964 - +1.3 881,967 + +1.4 881,971 - +1.5 881,971 - -2.7 881,972 + +2.8 881,972 + +1.4 881,979 - -0.1 881,980 - +1.7 882,016 + -1.5 882,066 - -0.3 882,113 - PGA1_c08550 0.12 +0.9 882,179 + PGA1_c08550 0.35 -3.1 882,188 + PGA1_c08550 0.38 -1.7 882,348 + +0.8 882,391 + -0.9 882,398 + +1.9 882,408 + -2.1 882,472 - -1.7 882,525 + +0.4 882,539 + +1.2 882,560 - +2.2 882,567 + -0.3 882,567 + -0.8 882,575 - +2.6 882,575 - -2.6 882,575 - +2.4 882,592 + +0.4 882,593 + -1.6 882,606 - -1.3 882,639 - +0.3 882,668 + -1.7 882,692 - PGA1_c08560 0.13 +2.4 882,849 - PGA1_c08560 0.36 -1.4 882,898 + PGA1_c08560 0.43 +2.2 882,898 + PGA1_c08560 0.43 +0.8 882,946 + PGA1_c08560 0.50 -0.1 882,952 + PGA1_c08560 0.51 -1.9 882,960 + PGA1_c08560 0.52 -1.1 882,960 - PGA1_c08560 0.52 +1.4
Or see this region's nucleotide sequence