Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00760 and PGA1_c00770 are separated by 158 nucleotides PGA1_c00770 and PGA1_c00780 are separated by 123 nucleotides PGA1_c00780 and PGA1_c00790 are separated by 58 nucleotides PGA1_c00790 and PGA1_c00800 are separated by 63 nucleotides
PGA1_c00760: PGA1_c00760 - putative pyruvate dehydrogenase complex repressor, at 73,699 to 74,469
_c00760
PGA1_c00770: PGA1_c00770 - hypothetical protein, at 74,628 to 74,966
_c00770
PGA1_c00780: PGA1_c00780 - putative chromosome segregation protein SMC, at 75,090 to 78,545
_c00780
PGA1_c00790: PGA1_c00790 - hypothetical protein, at 78,604 to 79,071
_c00790
PGA1_c00800: PGA1_c00800 - hypothetical protein, at 79,135 to 79,524
_c00800
Position (kb)
75
76
77
78
79 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 74.231 kb on + strand, within PGA1_c00760 at 74.246 kb on - strand, within PGA1_c00760 at 74.346 kb on + strand, within PGA1_c00760 at 74.363 kb on + strand, within PGA1_c00760 at 74.364 kb on - strand, within PGA1_c00760 at 74.381 kb on + strand, within PGA1_c00760 at 74.381 kb on + strand, within PGA1_c00760 at 74.381 kb on + strand, within PGA1_c00760 at 74.389 kb on - strand, within PGA1_c00760 at 74.400 kb on + strand at 74.424 kb on + strand at 74.424 kb on + strand at 74.432 kb on - strand at 74.432 kb on - strand at 74.437 kb on + strand at 74.448 kb on + strand at 74.473 kb on - strand at 74.473 kb on - strand at 74.504 kb on - strand at 74.525 kb on - strand at 74.525 kb on - strand at 74.535 kb on + strand at 74.536 kb on - strand at 74.536 kb on - strand at 74.536 kb on - strand at 74.573 kb on + strand at 74.586 kb on - strand at 74.619 kb on + strand at 74.619 kb on + strand at 74.619 kb on + strand at 74.622 kb on + strand at 74.622 kb on - strand at 74.627 kb on - strand at 74.628 kb on - strand at 74.628 kb on - strand at 74.628 kb on - strand at 74.629 kb on + strand at 74.647 kb on - strand at 74.660 kb on - strand at 74.696 kb on - strand, within PGA1_c00770 at 74.710 kb on + strand, within PGA1_c00770 at 74.754 kb on + strand, within PGA1_c00770 at 74.754 kb on - strand, within PGA1_c00770 at 74.762 kb on - strand, within PGA1_c00770 at 74.764 kb on - strand, within PGA1_c00770 at 74.775 kb on - strand, within PGA1_c00770 at 74.801 kb on + strand, within PGA1_c00770 at 74.834 kb on + strand, within PGA1_c00770 at 74.892 kb on - strand, within PGA1_c00770 at 74.892 kb on - strand, within PGA1_c00770 at 74.901 kb on + strand, within PGA1_c00770 at 74.901 kb on + strand, within PGA1_c00770 at 74.909 kb on - strand, within PGA1_c00770 at 74.909 kb on - strand, within PGA1_c00770 at 74.948 kb on + strand at 75.001 kb on - strand at 75.012 kb on + strand at 75.200 kb on + strand at 75.224 kb on - strand at 75.307 kb on - strand at 75.333 kb on - strand at 75.404 kb on - strand at 75.445 kb on + strand, within PGA1_c00780 at 75.445 kb on + strand, within PGA1_c00780 at 75.513 kb on + strand, within PGA1_c00780 at 75.822 kb on - strand, within PGA1_c00780 at 75.829 kb on - strand, within PGA1_c00780 at 75.847 kb on - strand, within PGA1_c00780 at 75.878 kb on - strand, within PGA1_c00780 at 75.981 kb on + strand, within PGA1_c00780 at 76.066 kb on - strand, within PGA1_c00780 at 76.126 kb on + strand, within PGA1_c00780 at 76.126 kb on + strand, within PGA1_c00780 at 76.126 kb on + strand, within PGA1_c00780 at 76.134 kb on - strand, within PGA1_c00780 at 76.153 kb on - strand, within PGA1_c00780 at 76.155 kb on + strand, within PGA1_c00780 at 76.293 kb on - strand, within PGA1_c00780 at 76.303 kb on - strand, within PGA1_c00780 at 76.303 kb on - strand, within PGA1_c00780 at 76.309 kb on - strand, within PGA1_c00780 at 76.388 kb on + strand, within PGA1_c00780 at 76.394 kb on + strand, within PGA1_c00780 at 76.542 kb on - strand, within PGA1_c00780 at 76.626 kb on + strand, within PGA1_c00780 at 76.680 kb on - strand, within PGA1_c00780 at 76.691 kb on + strand, within PGA1_c00780 at 76.800 kb on - strand, within PGA1_c00780 at 76.888 kb on + strand, within PGA1_c00780 at 76.899 kb on + strand, within PGA1_c00780 at 76.909 kb on + strand, within PGA1_c00780 at 76.917 kb on - strand, within PGA1_c00780 at 76.959 kb on - strand, within PGA1_c00780 at 77.054 kb on + strand, within PGA1_c00780 at 77.054 kb on + strand, within PGA1_c00780 at 77.054 kb on + strand, within PGA1_c00780 at 77.062 kb on - strand, within PGA1_c00780 at 77.104 kb on - strand, within PGA1_c00780 at 77.208 kb on - strand, within PGA1_c00780 at 77.426 kb on + strand, within PGA1_c00780 at 77.428 kb on + strand, within PGA1_c00780 at 77.434 kb on - strand, within PGA1_c00780 at 77.434 kb on - strand, within PGA1_c00780 at 77.483 kb on + strand, within PGA1_c00780 at 77.491 kb on - strand, within PGA1_c00780 at 77.559 kb on + strand, within PGA1_c00780 at 77.559 kb on + strand, within PGA1_c00780 at 77.567 kb on - strand, within PGA1_c00780 at 77.603 kb on - strand, within PGA1_c00780 at 77.635 kb on - strand, within PGA1_c00780 at 77.635 kb on - strand, within PGA1_c00780 at 77.641 kb on + strand, within PGA1_c00780 at 77.678 kb on - strand, within PGA1_c00780 at 77.751 kb on - strand, within PGA1_c00780 at 77.751 kb on - strand, within PGA1_c00780 at 77.775 kb on + strand, within PGA1_c00780 at 77.808 kb on + strand, within PGA1_c00780 at 77.813 kb on + strand, within PGA1_c00780 at 77.816 kb on - strand, within PGA1_c00780 at 77.816 kb on - strand, within PGA1_c00780 at 77.840 kb on - strand, within PGA1_c00780 at 77.850 kb on - strand, within PGA1_c00780 at 77.856 kb on + strand, within PGA1_c00780 at 77.864 kb on - strand, within PGA1_c00780 at 77.902 kb on - strand, within PGA1_c00780 at 77.940 kb on - strand, within PGA1_c00780 at 78.074 kb on + strand, within PGA1_c00780 at 78.085 kb on + strand, within PGA1_c00780 at 78.093 kb on - strand, within PGA1_c00780 at 78.109 kb on + strand, within PGA1_c00780 at 78.151 kb on + strand, within PGA1_c00780 at 78.276 kb on + strand at 78.284 kb on - strand at 78.309 kb on + strand at 78.506 kb on - strand at 78.541 kb on + strand at 78.568 kb on + strand at 78.637 kb on - strand at 78.642 kb on - strand at 78.653 kb on + strand, within PGA1_c00790 at 78.661 kb on - strand, within PGA1_c00790 at 78.661 kb on - strand, within PGA1_c00790 at 78.661 kb on - strand, within PGA1_c00790 at 78.678 kb on + strand, within PGA1_c00790 at 78.740 kb on + strand, within PGA1_c00790 at 78.772 kb on + strand, within PGA1_c00790 at 78.803 kb on + strand, within PGA1_c00790 at 78.804 kb on + strand, within PGA1_c00790 at 78.811 kb on - strand, within PGA1_c00790 at 78.859 kb on - strand, within PGA1_c00790 at 78.935 kb on - strand, within PGA1_c00790 at 79.037 kb on - strand at 79.065 kb on + strand at 79.075 kb on + strand at 79.075 kb on + strand at 79.075 kb on + strand at 79.083 kb on - strand at 79.083 kb on - strand at 79.133 kb on + strand at 79.186 kb on + strand, within PGA1_c00800 at 79.209 kb on + strand, within PGA1_c00800 at 79.227 kb on - strand, within PGA1_c00800 at 79.244 kb on - strand, within PGA1_c00800 at 79.308 kb on + strand, within PGA1_c00800 at 79.366 kb on + strand, within PGA1_c00800 at 79.374 kb on - strand, within PGA1_c00800 at 79.374 kb on - strand, within PGA1_c00800 at 79.382 kb on - strand, within PGA1_c00800 at 79.428 kb on + strand, within PGA1_c00800 at 79.458 kb on + strand, within PGA1_c00800 at 79.518 kb on + strand at 79.526 kb on - strand at 79.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 74,231 + PGA1_c00760 0.69 -2.3 74,246 - PGA1_c00760 0.71 -1.3 74,346 + PGA1_c00760 0.84 -1.0 74,363 + PGA1_c00760 0.86 -2.3 74,364 - PGA1_c00760 0.86 -1.9 74,381 + PGA1_c00760 0.88 +2.1 74,381 + PGA1_c00760 0.88 +1.9 74,381 + PGA1_c00760 0.88 +3.2 74,389 - PGA1_c00760 0.89 +0.3 74,400 + -2.4 74,424 + -1.1 74,424 + -0.4 74,432 - +3.8 74,432 - -1.2 74,437 + -0.8 74,448 + -1.0 74,473 - +1.5 74,473 - -1.4 74,504 - -2.9 74,525 - +1.5 74,525 - +2.0 74,535 + +2.2 74,536 - -2.2 74,536 - -0.9 74,536 - +0.4 74,573 + -1.7 74,586 - -0.8 74,619 + -2.6 74,619 + -1.0 74,619 + +1.1 74,622 + -1.8 74,622 - -0.6 74,627 - -2.4 74,628 - +1.8 74,628 - -0.7 74,628 - -2.4 74,629 + +3.5 74,647 - +2.4 74,660 - +0.8 74,696 - PGA1_c00770 0.20 -1.4 74,710 + PGA1_c00770 0.24 +1.8 74,754 + PGA1_c00770 0.37 +1.9 74,754 - PGA1_c00770 0.37 -1.6 74,762 - PGA1_c00770 0.40 -3.1 74,764 - PGA1_c00770 0.40 -0.8 74,775 - PGA1_c00770 0.43 +1.5 74,801 + PGA1_c00770 0.51 -2.0 74,834 + PGA1_c00770 0.61 -0.3 74,892 - PGA1_c00770 0.78 -1.6 74,892 - PGA1_c00770 0.78 -1.2 74,901 + PGA1_c00770 0.81 +3.0 74,901 + PGA1_c00770 0.81 +2.1 74,909 - PGA1_c00770 0.83 -0.1 74,909 - PGA1_c00770 0.83 -1.1 74,948 + +2.3 75,001 - -1.5 75,012 + -1.2 75,200 + -1.8 75,224 - -2.9 75,307 - -0.7 75,333 - -0.4 75,404 - +1.8 75,445 + PGA1_c00780 0.10 -0.9 75,445 + PGA1_c00780 0.10 -0.8 75,513 + PGA1_c00780 0.12 -3.0 75,822 - PGA1_c00780 0.21 +1.6 75,829 - PGA1_c00780 0.21 -2.1 75,847 - PGA1_c00780 0.22 +0.6 75,878 - PGA1_c00780 0.23 -0.4 75,981 + PGA1_c00780 0.26 +0.4 76,066 - PGA1_c00780 0.28 -1.0 76,126 + PGA1_c00780 0.30 +0.6 76,126 + PGA1_c00780 0.30 -1.0 76,126 + PGA1_c00780 0.30 +1.8 76,134 - PGA1_c00780 0.30 -0.5 76,153 - PGA1_c00780 0.31 -2.9 76,155 + PGA1_c00780 0.31 -1.6 76,293 - PGA1_c00780 0.35 +0.5 76,303 - PGA1_c00780 0.35 -0.4 76,303 - PGA1_c00780 0.35 +1.1 76,309 - PGA1_c00780 0.35 -1.9 76,388 + PGA1_c00780 0.38 +1.8 76,394 + PGA1_c00780 0.38 +0.6 76,542 - PGA1_c00780 0.42 -1.5 76,626 + PGA1_c00780 0.44 +0.1 76,680 - PGA1_c00780 0.46 -0.4 76,691 + PGA1_c00780 0.46 -1.8 76,800 - PGA1_c00780 0.49 -1.5 76,888 + PGA1_c00780 0.52 +0.3 76,899 + PGA1_c00780 0.52 -2.6 76,909 + PGA1_c00780 0.53 -2.6 76,917 - PGA1_c00780 0.53 +1.7 76,959 - PGA1_c00780 0.54 +1.1 77,054 + PGA1_c00780 0.57 -1.4 77,054 + PGA1_c00780 0.57 -0.8 77,054 + PGA1_c00780 0.57 -2.0 77,062 - PGA1_c00780 0.57 -2.0 77,104 - PGA1_c00780 0.58 -0.4 77,208 - PGA1_c00780 0.61 -1.6 77,426 + PGA1_c00780 0.68 -3.0 77,428 + PGA1_c00780 0.68 -1.8 77,434 - PGA1_c00780 0.68 -2.0 77,434 - PGA1_c00780 0.68 -0.4 77,483 + PGA1_c00780 0.69 -2.1 77,491 - PGA1_c00780 0.69 -0.8 77,559 + PGA1_c00780 0.71 -0.1 77,559 + PGA1_c00780 0.71 -0.6 77,567 - PGA1_c00780 0.72 +1.6 77,603 - PGA1_c00780 0.73 -0.2 77,635 - PGA1_c00780 0.74 -1.5 77,635 - PGA1_c00780 0.74 -1.8 77,641 + PGA1_c00780 0.74 -1.2 77,678 - PGA1_c00780 0.75 -1.2 77,751 - PGA1_c00780 0.77 +0.8 77,751 - PGA1_c00780 0.77 +0.3 77,775 + PGA1_c00780 0.78 -2.0 77,808 + PGA1_c00780 0.79 -1.8 77,813 + PGA1_c00780 0.79 -0.5 77,816 - PGA1_c00780 0.79 -1.8 77,816 - PGA1_c00780 0.79 -1.7 77,840 - PGA1_c00780 0.80 -1.8 77,850 - PGA1_c00780 0.80 -0.5 77,856 + PGA1_c00780 0.80 -2.3 77,864 - PGA1_c00780 0.80 -1.5 77,902 - PGA1_c00780 0.81 +2.6 77,940 - PGA1_c00780 0.82 -2.4 78,074 + PGA1_c00780 0.86 +0.2 78,085 + PGA1_c00780 0.87 +0.2 78,093 - PGA1_c00780 0.87 -1.2 78,109 + PGA1_c00780 0.87 -1.9 78,151 + PGA1_c00780 0.89 -1.7 78,276 + -3.3 78,284 - -2.6 78,309 + -0.8 78,506 - -0.7 78,541 + +0.1 78,568 + -1.6 78,637 - +3.3 78,642 - -1.1 78,653 + PGA1_c00790 0.10 -1.9 78,661 - PGA1_c00790 0.12 +0.2 78,661 - PGA1_c00790 0.12 +3.4 78,661 - PGA1_c00790 0.12 -1.0 78,678 + PGA1_c00790 0.16 -0.6 78,740 + PGA1_c00790 0.29 -1.3 78,772 + PGA1_c00790 0.36 +3.2 78,803 + PGA1_c00790 0.43 +1.8 78,804 + PGA1_c00790 0.43 +1.8 78,811 - PGA1_c00790 0.44 +1.0 78,859 - PGA1_c00790 0.54 +1.3 78,935 - PGA1_c00790 0.71 +2.0 79,037 - +2.8 79,065 + -1.4 79,075 + -1.6 79,075 + -0.8 79,075 + -0.9 79,083 - -1.4 79,083 - -0.3 79,133 + -2.6 79,186 + PGA1_c00800 0.13 +2.8 79,209 + PGA1_c00800 0.19 +1.3 79,227 - PGA1_c00800 0.24 -0.4 79,244 - PGA1_c00800 0.28 -0.9 79,308 + PGA1_c00800 0.44 -1.0 79,366 + PGA1_c00800 0.59 -1.0 79,374 - PGA1_c00800 0.61 +0.3 79,374 - PGA1_c00800 0.61 -0.8 79,382 - PGA1_c00800 0.63 -2.8 79,428 + PGA1_c00800 0.75 +1.5 79,458 + PGA1_c00800 0.83 -2.2 79,518 + -2.2 79,526 - +1.1 79,539 + -1.9
Or see this region's nucleotide sequence