Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c07030 and PGA1_c07040 are separated by 71 nucleotides PGA1_c07040 and PGA1_c07050 are separated by 87 nucleotides PGA1_c07050 and PGA1_c07060 are separated by 165 nucleotides
PGA1_c07030: PGA1_c07030 - phenylalanyl-tRNA synthetase beta chain, at 721,663 to 724,059
_c07030
PGA1_c07040: PGA1_c07040 - transcriptional regulator, lysR family, at 724,131 to 725,009
_c07040
PGA1_c07050: PGA1_c07050 - methyltransferase domain-containing protein, at 725,097 to 725,732
_c07050
PGA1_c07060: PGA1_c07060 - YtoQ family protein, at 725,898 to 726,347
_c07060
Position (kb) 724
725
726 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 724.087 kb on - strand at 724.160 kb on + strand at 724.160 kb on + strand at 724.160 kb on + strand at 724.168 kb on - strand at 724.168 kb on - strand at 724.168 kb on - strand at 724.168 kb on - strand at 724.235 kb on + strand, within PGA1_c07040 at 724.243 kb on - strand, within PGA1_c07040 at 724.243 kb on - strand, within PGA1_c07040 at 724.244 kb on - strand, within PGA1_c07040 at 724.246 kb on + strand, within PGA1_c07040 at 724.254 kb on - strand, within PGA1_c07040 at 724.277 kb on - strand, within PGA1_c07040 at 724.333 kb on - strand, within PGA1_c07040 at 724.383 kb on - strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.455 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.504 kb on + strand, within PGA1_c07040 at 724.530 kb on + strand, within PGA1_c07040 at 724.564 kb on - strand, within PGA1_c07040 at 724.594 kb on + strand, within PGA1_c07040 at 724.656 kb on + strand, within PGA1_c07040 at 724.675 kb on + strand, within PGA1_c07040 at 724.910 kb on - strand, within PGA1_c07040 at 724.912 kb on + strand, within PGA1_c07040 at 724.915 kb on - strand, within PGA1_c07040 at 724.918 kb on + strand, within PGA1_c07040 at 724.922 kb on + strand at 724.922 kb on + strand at 724.922 kb on + strand at 724.930 kb on - strand at 724.930 kb on - strand at 724.937 kb on + strand at 724.937 kb on + strand at 724.941 kb on + strand at 724.947 kb on + strand at 724.978 kb on + strand at 725.147 kb on + strand at 725.192 kb on + strand, within PGA1_c07050 at 725.197 kb on + strand, within PGA1_c07050 at 725.200 kb on - strand, within PGA1_c07050 at 725.200 kb on - strand, within PGA1_c07050 at 725.200 kb on - strand, within PGA1_c07050 at 725.205 kb on - strand, within PGA1_c07050 at 725.211 kb on - strand, within PGA1_c07050 at 725.219 kb on - strand, within PGA1_c07050 at 725.275 kb on - strand, within PGA1_c07050 at 725.325 kb on + strand, within PGA1_c07050 at 725.330 kb on + strand, within PGA1_c07050 at 725.338 kb on - strand, within PGA1_c07050 at 725.373 kb on - strand, within PGA1_c07050 at 725.390 kb on - strand, within PGA1_c07050 at 725.422 kb on + strand, within PGA1_c07050 at 725.430 kb on - strand, within PGA1_c07050 at 725.489 kb on - strand, within PGA1_c07050 at 725.532 kb on + strand, within PGA1_c07050 at 725.597 kb on + strand, within PGA1_c07050 at 725.751 kb on + strand at 725.769 kb on - strand at 725.769 kb on - strand at 725.793 kb on - strand at 725.802 kb on + strand at 725.836 kb on + strand at 725.867 kb on + strand at 725.954 kb on - strand, within PGA1_c07060 at 725.984 kb on + strand, within PGA1_c07060 at 725.992 kb on - strand, within PGA1_c07060
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 724,087 - +2.6 724,160 + -1.6 724,160 + +0.5 724,160 + -1.1 724,168 - -1.2 724,168 - +2.0 724,168 - +2.1 724,168 - +2.4 724,235 + PGA1_c07040 0.12 -2.7 724,243 - PGA1_c07040 0.13 -2.0 724,243 - PGA1_c07040 0.13 +2.7 724,244 - PGA1_c07040 0.13 -0.8 724,246 + PGA1_c07040 0.13 +1.2 724,254 - PGA1_c07040 0.14 -1.6 724,277 - PGA1_c07040 0.17 +2.4 724,333 - PGA1_c07040 0.23 -0.3 724,383 - PGA1_c07040 0.29 +1.9 724,416 + PGA1_c07040 0.32 -1.9 724,416 + PGA1_c07040 0.32 -1.3 724,416 + PGA1_c07040 0.32 +1.7 724,416 + PGA1_c07040 0.32 -2.9 724,424 - PGA1_c07040 0.33 -1.6 724,424 - PGA1_c07040 0.33 +0.9 724,424 - PGA1_c07040 0.33 -1.0 724,424 - PGA1_c07040 0.33 +2.0 724,455 + PGA1_c07040 0.37 -2.5 724,494 + PGA1_c07040 0.41 -0.1 724,494 + PGA1_c07040 0.41 -2.7 724,494 + PGA1_c07040 0.41 +2.3 724,494 + PGA1_c07040 0.41 +0.5 724,504 + PGA1_c07040 0.42 -0.2 724,530 + PGA1_c07040 0.45 -2.9 724,564 - PGA1_c07040 0.49 -0.2 724,594 + PGA1_c07040 0.53 +1.4 724,656 + PGA1_c07040 0.60 -1.6 724,675 + PGA1_c07040 0.62 +2.3 724,910 - PGA1_c07040 0.89 -1.9 724,912 + PGA1_c07040 0.89 +1.6 724,915 - PGA1_c07040 0.89 +2.0 724,918 + PGA1_c07040 0.90 -2.8 724,922 + +3.2 724,922 + +1.6 724,922 + -1.4 724,930 - -1.7 724,930 - -0.2 724,937 + +1.1 724,937 + -2.5 724,941 + -2.2 724,947 + -1.6 724,978 + +3.1 725,147 + +2.3 725,192 + PGA1_c07050 0.15 +1.4 725,197 + PGA1_c07050 0.16 +1.5 725,200 - PGA1_c07050 0.16 -1.9 725,200 - PGA1_c07050 0.16 -2.4 725,200 - PGA1_c07050 0.16 +1.3 725,205 - PGA1_c07050 0.17 -2.6 725,211 - PGA1_c07050 0.18 -1.6 725,219 - PGA1_c07050 0.19 -2.1 725,275 - PGA1_c07050 0.28 +2.6 725,325 + PGA1_c07050 0.36 -2.9 725,330 + PGA1_c07050 0.37 -2.7 725,338 - PGA1_c07050 0.38 +2.1 725,373 - PGA1_c07050 0.43 -2.5 725,390 - PGA1_c07050 0.46 -1.2 725,422 + PGA1_c07050 0.51 +1.5 725,430 - PGA1_c07050 0.52 -1.6 725,489 - PGA1_c07050 0.62 -1.6 725,532 + PGA1_c07050 0.68 -2.5 725,597 + PGA1_c07050 0.79 +2.1 725,751 + -0.3 725,769 - -1.2 725,769 - -1.5 725,793 - -0.5 725,802 + +3.0 725,836 + +1.3 725,867 + +1.9 725,954 - PGA1_c07060 0.12 -1.2 725,984 + PGA1_c07060 0.19 -1.7 725,992 - PGA1_c07060 0.21 -1.7
Or see this region's nucleotide sequence