Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00650 and PGA1_c00660 are separated by 106 nucleotides PGA1_c00660 and PGA1_c00670 overlap by 4 nucleotides PGA1_c00670 and PGA1_c00680 are separated by 6 nucleotides PGA1_c00680 and PGA1_c00690 are separated by 96 nucleotides
PGA1_c00650: PGA1_c00650 - Uncharacterized conserved protein, at 66,112 to 66,639
_c00650
PGA1_c00660: PGA1_c00660 - transcriptional regulator, lysR family, at 66,746 to 67,624
_c00660
PGA1_c00670: PGA1_c00670 - putative integral membrane protein, at 67,621 to 68,529
_c00670
PGA1_c00680: PGA1_c00680 - transcriptional regulator, arsR family, at 68,536 to 68,883
_c00680
PGA1_c00690: PGA1_c00690 - putative integral membrane protein, at 68,980 to 69,873
_c00690
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 66.641 kb on - strand at 66.641 kb on - strand at 66.641 kb on - strand at 66.641 kb on - strand at 66.705 kb on + strand at 66.730 kb on - strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.732 kb on + strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.740 kb on - strand at 66.745 kb on - strand at 66.771 kb on - strand at 66.850 kb on - strand, within PGA1_c00660 at 66.871 kb on - strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.955 kb on + strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.963 kb on - strand, within PGA1_c00660 at 66.991 kb on + strand, within PGA1_c00660 at 66.998 kb on + strand, within PGA1_c00660 at 66.999 kb on + strand, within PGA1_c00660 at 67.102 kb on - strand, within PGA1_c00660 at 67.208 kb on + strand, within PGA1_c00660 at 67.238 kb on + strand, within PGA1_c00660 at 67.267 kb on + strand, within PGA1_c00660 at 67.284 kb on - strand, within PGA1_c00660 at 67.291 kb on - strand, within PGA1_c00660 at 67.329 kb on - strand, within PGA1_c00660 at 67.421 kb on - strand, within PGA1_c00660 at 67.429 kb on + strand, within PGA1_c00660 at 67.429 kb on + strand, within PGA1_c00660 at 67.437 kb on - strand, within PGA1_c00660 at 67.459 kb on - strand, within PGA1_c00660 at 67.487 kb on - strand, within PGA1_c00660 at 67.498 kb on - strand, within PGA1_c00660 at 67.531 kb on - strand, within PGA1_c00660 at 67.550 kb on + strand at 67.550 kb on + strand at 67.558 kb on - strand at 67.571 kb on - strand at 67.595 kb on - strand at 67.609 kb on + strand at 67.609 kb on + strand at 67.617 kb on - strand at 67.617 kb on - strand at 67.642 kb on - strand at 67.659 kb on - strand at 67.694 kb on + strand at 67.717 kb on + strand, within PGA1_c00670 at 67.718 kb on + strand, within PGA1_c00670 at 67.721 kb on - strand, within PGA1_c00670 at 67.728 kb on + strand, within PGA1_c00670 at 67.728 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.787 kb on + strand, within PGA1_c00670 at 67.788 kb on - strand, within PGA1_c00670 at 67.792 kb on + strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.795 kb on - strand, within PGA1_c00670 at 67.864 kb on + strand, within PGA1_c00670 at 67.902 kb on - strand, within PGA1_c00670 at 67.907 kb on + strand, within PGA1_c00670 at 67.912 kb on + strand, within PGA1_c00670 at 67.940 kb on - strand, within PGA1_c00670 at 67.971 kb on + strand, within PGA1_c00670 at 67.990 kb on + strand, within PGA1_c00670 at 67.990 kb on + strand, within PGA1_c00670 at 67.990 kb on + strand, within PGA1_c00670 at 67.990 kb on + strand, within PGA1_c00670 at 67.990 kb on + strand, within PGA1_c00670 at 67.998 kb on - strand, within PGA1_c00670 at 67.998 kb on - strand, within PGA1_c00670 at 68.046 kb on + strand, within PGA1_c00670 at 68.109 kb on + strand, within PGA1_c00670 at 68.120 kb on + strand, within PGA1_c00670 at 68.147 kb on + strand, within PGA1_c00670 at 68.167 kb on + strand, within PGA1_c00670 at 68.255 kb on + strand, within PGA1_c00670 at 68.288 kb on - strand, within PGA1_c00670 at 68.288 kb on - strand, within PGA1_c00670 at 68.288 kb on - strand, within PGA1_c00670 at 68.288 kb on - strand, within PGA1_c00670 at 68.303 kb on - strand, within PGA1_c00670 at 68.312 kb on + strand, within PGA1_c00670 at 68.312 kb on + strand, within PGA1_c00670 at 68.312 kb on + strand, within PGA1_c00670 at 68.330 kb on + strand, within PGA1_c00670 at 68.344 kb on - strand, within PGA1_c00670 at 68.354 kb on - strand, within PGA1_c00670 at 68.358 kb on + strand, within PGA1_c00670 at 68.366 kb on - strand, within PGA1_c00670 at 68.366 kb on - strand, within PGA1_c00670 at 68.366 kb on - strand, within PGA1_c00670 at 68.366 kb on - strand, within PGA1_c00670 at 68.367 kb on - strand, within PGA1_c00670 at 68.373 kb on - strand, within PGA1_c00670 at 68.375 kb on + strand, within PGA1_c00670 at 68.375 kb on + strand, within PGA1_c00670 at 68.377 kb on - strand, within PGA1_c00670 at 68.377 kb on - strand, within PGA1_c00670 at 68.384 kb on - strand, within PGA1_c00670 at 68.418 kb on - strand, within PGA1_c00670 at 68.423 kb on - strand, within PGA1_c00670 at 68.514 kb on + strand at 68.532 kb on - strand at 68.548 kb on + strand at 68.553 kb on - strand at 68.578 kb on - strand, within PGA1_c00680 at 68.584 kb on - strand, within PGA1_c00680 at 68.612 kb on + strand, within PGA1_c00680 at 68.835 kb on + strand, within PGA1_c00680 at 68.891 kb on + strand at 68.899 kb on - strand at 68.899 kb on - strand at 68.984 kb on - strand at 68.991 kb on + strand at 69.006 kb on - strand at 69.028 kb on + strand at 69.073 kb on - strand, within PGA1_c00690 at 69.076 kb on + strand, within PGA1_c00690 at 69.078 kb on + strand, within PGA1_c00690 at 69.109 kb on + strand, within PGA1_c00690 at 69.131 kb on - strand, within PGA1_c00690 at 69.201 kb on + strand, within PGA1_c00690 at 69.201 kb on - strand, within PGA1_c00690 at 69.238 kb on - strand, within PGA1_c00690 at 69.246 kb on - strand, within PGA1_c00690 at 69.292 kb on - strand, within PGA1_c00690 at 69.320 kb on - strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.322 kb on + strand, within PGA1_c00690 at 69.323 kb on + strand, within PGA1_c00690 at 69.330 kb on - strand, within PGA1_c00690 at 69.330 kb on - strand, within PGA1_c00690 at 69.330 kb on - strand, within PGA1_c00690 at 69.330 kb on - strand, within PGA1_c00690 at 69.378 kb on + strand, within PGA1_c00690 at 69.419 kb on + strand, within PGA1_c00690 at 69.419 kb on + strand, within PGA1_c00690 at 69.426 kb on + strand, within PGA1_c00690 at 69.426 kb on + strand, within PGA1_c00690 at 69.426 kb on + strand, within PGA1_c00690 at 69.426 kb on + strand, within PGA1_c00690 at 69.427 kb on - strand, within PGA1_c00690 at 69.434 kb on - strand, within PGA1_c00690 at 69.434 kb on - strand, within PGA1_c00690 at 69.434 kb on - strand, within PGA1_c00690 at 69.434 kb on - strand, within PGA1_c00690 at 69.457 kb on + strand, within PGA1_c00690 at 69.457 kb on + strand, within PGA1_c00690 at 69.457 kb on + strand, within PGA1_c00690 at 69.457 kb on + strand, within PGA1_c00690 at 69.462 kb on + strand, within PGA1_c00690 at 69.465 kb on - strand, within PGA1_c00690 at 69.465 kb on - strand, within PGA1_c00690
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 66,641 - +2.3 66,641 - +3.7 66,641 - -1.4 66,641 - +3.2 66,705 + +1.3 66,730 - +1.5 66,732 + +1.5 66,732 + +2.4 66,732 + +0.7 66,732 + +2.1 66,732 + +0.4 66,732 + -1.3 66,732 + +0.5 66,740 - -0.9 66,740 - -2.4 66,740 - +1.8 66,740 - -1.2 66,740 - -1.6 66,740 - -0.2 66,740 - -2.5 66,740 - +1.4 66,740 - +0.6 66,740 - -2.3 66,745 - -2.9 66,771 - +2.4 66,850 - PGA1_c00660 0.12 +3.3 66,871 - PGA1_c00660 0.14 -1.9 66,955 + PGA1_c00660 0.24 +2.0 66,955 + PGA1_c00660 0.24 -1.2 66,955 + PGA1_c00660 0.24 +0.3 66,955 + PGA1_c00660 0.24 +1.6 66,963 - PGA1_c00660 0.25 +0.7 66,963 - PGA1_c00660 0.25 +1.1 66,963 - PGA1_c00660 0.25 -3.0 66,963 - PGA1_c00660 0.25 -1.2 66,991 + PGA1_c00660 0.28 +1.1 66,998 + PGA1_c00660 0.29 -1.7 66,999 + PGA1_c00660 0.29 -2.2 67,102 - PGA1_c00660 0.41 +0.5 67,208 + PGA1_c00660 0.53 +2.4 67,238 + PGA1_c00660 0.56 +1.1 67,267 + PGA1_c00660 0.59 +3.3 67,284 - PGA1_c00660 0.61 +2.4 67,291 - PGA1_c00660 0.62 -2.1 67,329 - PGA1_c00660 0.66 -1.8 67,421 - PGA1_c00660 0.77 -1.9 67,429 + PGA1_c00660 0.78 -2.7 67,429 + PGA1_c00660 0.78 +1.1 67,437 - PGA1_c00660 0.79 -1.4 67,459 - PGA1_c00660 0.81 +0.2 67,487 - PGA1_c00660 0.84 +3.0 67,498 - PGA1_c00660 0.86 -1.7 67,531 - PGA1_c00660 0.89 -2.7 67,550 + -2.8 67,550 + +1.2 67,558 - -1.7 67,571 - +0.4 67,595 - -1.4 67,609 + -1.7 67,609 + -0.5 67,617 - +1.2 67,617 - -0.1 67,642 - +0.7 67,659 - -2.4 67,694 + -1.1 67,717 + PGA1_c00670 0.11 +2.1 67,718 + PGA1_c00670 0.11 -3.1 67,721 - PGA1_c00670 0.11 +1.5 67,728 + PGA1_c00670 0.12 -0.8 67,728 + PGA1_c00670 0.12 +0.1 67,787 + PGA1_c00670 0.18 +1.9 67,787 + PGA1_c00670 0.18 -1.3 67,787 + PGA1_c00670 0.18 +0.3 67,787 + PGA1_c00670 0.18 -1.9 67,787 + PGA1_c00670 0.18 +4.4 67,787 + PGA1_c00670 0.18 +2.0 67,787 + PGA1_c00670 0.18 -1.3 67,787 + PGA1_c00670 0.18 -2.1 67,787 + PGA1_c00670 0.18 +1.9 67,787 + PGA1_c00670 0.18 +0.6 67,787 + PGA1_c00670 0.18 +1.0 67,787 + PGA1_c00670 0.18 -1.9 67,787 + PGA1_c00670 0.18 -3.5 67,787 + PGA1_c00670 0.18 -0.8 67,788 - PGA1_c00670 0.18 +0.3 67,792 + PGA1_c00670 0.19 -2.8 67,795 - PGA1_c00670 0.19 -2.8 67,795 - PGA1_c00670 0.19 -2.1 67,795 - PGA1_c00670 0.19 -2.5 67,795 - PGA1_c00670 0.19 -1.8 67,795 - PGA1_c00670 0.19 -0.2 67,795 - PGA1_c00670 0.19 -0.7 67,795 - PGA1_c00670 0.19 -0.7 67,795 - PGA1_c00670 0.19 +2.5 67,795 - PGA1_c00670 0.19 +1.5 67,795 - PGA1_c00670 0.19 -2.9 67,795 - PGA1_c00670 0.19 -1.1 67,795 - PGA1_c00670 0.19 -1.2 67,795 - PGA1_c00670 0.19 +0.9 67,864 + PGA1_c00670 0.27 -1.3 67,902 - PGA1_c00670 0.31 +2.8 67,907 + PGA1_c00670 0.31 +1.0 67,912 + PGA1_c00670 0.32 -1.6 67,940 - PGA1_c00670 0.35 -0.7 67,971 + PGA1_c00670 0.39 -2.1 67,990 + PGA1_c00670 0.41 +2.1 67,990 + PGA1_c00670 0.41 -1.2 67,990 + PGA1_c00670 0.41 +1.1 67,990 + PGA1_c00670 0.41 -1.1 67,990 + PGA1_c00670 0.41 +0.2 67,998 - PGA1_c00670 0.41 -1.5 67,998 - PGA1_c00670 0.41 +1.4 68,046 + PGA1_c00670 0.47 -0.7 68,109 + PGA1_c00670 0.54 -1.1 68,120 + PGA1_c00670 0.55 -0.1 68,147 + PGA1_c00670 0.58 +2.9 68,167 + PGA1_c00670 0.60 -0.7 68,255 + PGA1_c00670 0.70 -2.0 68,288 - PGA1_c00670 0.73 +1.8 68,288 - PGA1_c00670 0.73 -2.7 68,288 - PGA1_c00670 0.73 +2.8 68,288 - PGA1_c00670 0.73 +3.6 68,303 - PGA1_c00670 0.75 -1.8 68,312 + PGA1_c00670 0.76 +0.3 68,312 + PGA1_c00670 0.76 +2.7 68,312 + PGA1_c00670 0.76 -1.8 68,330 + PGA1_c00670 0.78 +3.4 68,344 - PGA1_c00670 0.80 -2.1 68,354 - PGA1_c00670 0.81 -0.6 68,358 + PGA1_c00670 0.81 -1.9 68,366 - PGA1_c00670 0.82 -0.7 68,366 - PGA1_c00670 0.82 -1.9 68,366 - PGA1_c00670 0.82 -0.7 68,366 - PGA1_c00670 0.82 -1.4 68,367 - PGA1_c00670 0.82 +0.0 68,373 - PGA1_c00670 0.83 -1.8 68,375 + PGA1_c00670 0.83 -0.1 68,375 + PGA1_c00670 0.83 +0.3 68,377 - PGA1_c00670 0.83 +1.1 68,377 - PGA1_c00670 0.83 -2.8 68,384 - PGA1_c00670 0.84 +2.0 68,418 - PGA1_c00670 0.88 -2.0 68,423 - PGA1_c00670 0.88 -0.5 68,514 + +2.5 68,532 - -0.7 68,548 + -0.4 68,553 - +1.8 68,578 - PGA1_c00680 0.12 +0.7 68,584 - PGA1_c00680 0.14 +2.7 68,612 + PGA1_c00680 0.22 -0.9 68,835 + PGA1_c00680 0.86 +0.6 68,891 + +0.7 68,899 - +2.9 68,899 - -2.0 68,984 - -2.2 68,991 + -0.9 69,006 - -0.5 69,028 + +1.7 69,073 - PGA1_c00690 0.10 -1.2 69,076 + PGA1_c00690 0.11 -0.2 69,078 + PGA1_c00690 0.11 +0.3 69,109 + PGA1_c00690 0.14 +3.1 69,131 - PGA1_c00690 0.17 -2.0 69,201 + PGA1_c00690 0.25 -0.2 69,201 - PGA1_c00690 0.25 -1.4 69,238 - PGA1_c00690 0.29 -1.2 69,246 - PGA1_c00690 0.30 -1.7 69,292 - PGA1_c00690 0.35 -2.1 69,320 - PGA1_c00690 0.38 +3.0 69,322 + PGA1_c00690 0.38 +1.5 69,322 + PGA1_c00690 0.38 -2.1 69,322 + PGA1_c00690 0.38 +0.1 69,322 + PGA1_c00690 0.38 -1.8 69,322 + PGA1_c00690 0.38 -1.3 69,322 + PGA1_c00690 0.38 +1.0 69,322 + PGA1_c00690 0.38 -0.9 69,322 + PGA1_c00690 0.38 +1.2 69,322 + PGA1_c00690 0.38 +2.4 69,322 + PGA1_c00690 0.38 -1.5 69,322 + PGA1_c00690 0.38 -2.1 69,322 + PGA1_c00690 0.38 +0.7 69,322 + PGA1_c00690 0.38 +1.5 69,322 + PGA1_c00690 0.38 -0.9 69,322 + PGA1_c00690 0.38 -0.7 69,322 + PGA1_c00690 0.38 -0.2 69,322 + PGA1_c00690 0.38 -2.4 69,323 + PGA1_c00690 0.38 +1.4 69,330 - PGA1_c00690 0.39 +2.9 69,330 - PGA1_c00690 0.39 +0.3 69,330 - PGA1_c00690 0.39 -3.1 69,330 - PGA1_c00690 0.39 +2.6 69,378 + PGA1_c00690 0.45 -0.5 69,419 + PGA1_c00690 0.49 -0.7 69,419 + PGA1_c00690 0.49 +3.2 69,426 + PGA1_c00690 0.50 -2.2 69,426 + PGA1_c00690 0.50 +2.4 69,426 + PGA1_c00690 0.50 -3.3 69,426 + PGA1_c00690 0.50 +1.8 69,427 - PGA1_c00690 0.50 -3.2 69,434 - PGA1_c00690 0.51 +2.6 69,434 - PGA1_c00690 0.51 -1.8 69,434 - PGA1_c00690 0.51 -2.3 69,434 - PGA1_c00690 0.51 -1.3 69,457 + PGA1_c00690 0.53 +5.1 69,457 + PGA1_c00690 0.53 -0.4 69,457 + PGA1_c00690 0.53 -3.0 69,457 + PGA1_c00690 0.53 +1.7 69,462 + PGA1_c00690 0.54 -1.1 69,465 - PGA1_c00690 0.54 +0.7 69,465 - PGA1_c00690 0.54 +0.0
Or see this region's nucleotide sequence