Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06190 and PGA1_c06200 are separated by 64 nucleotides PGA1_c06200 and PGA1_c06210 are separated by 12 nucleotides PGA1_c06210 and PGA1_c06220 are separated by 254 nucleotides
PGA1_c06190: PGA1_c06190 - hypothetical protein, at 632,900 to 633,391
_c06190
PGA1_c06200: PGA1_c06200 - probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B, at 633,456 to 634,085
_c06200
PGA1_c06210: PGA1_c06210 - probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A, at 634,098 to 634,817
_c06210
PGA1_c06220: PGA1_c06220 - DNA topoisomerase TopA, at 635,072 to 637,774
_c06220
Position (kb) 634
635 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 633.103 kb on - strand, within PGA1_c06190 at 633.121 kb on + strand, within PGA1_c06190 at 633.137 kb on + strand, within PGA1_c06190 at 633.184 kb on - strand, within PGA1_c06190 at 633.201 kb on + strand, within PGA1_c06190 at 633.201 kb on + strand, within PGA1_c06190 at 633.201 kb on + strand, within PGA1_c06190 at 633.220 kb on + strand, within PGA1_c06190 at 633.220 kb on + strand, within PGA1_c06190 at 633.222 kb on + strand, within PGA1_c06190 at 633.228 kb on - strand, within PGA1_c06190 at 633.257 kb on - strand, within PGA1_c06190 at 633.444 kb on + strand at 633.476 kb on + strand at 633.484 kb on - strand at 633.565 kb on - strand, within PGA1_c06200 at 633.610 kb on - strand, within PGA1_c06200 at 633.695 kb on - strand, within PGA1_c06200 at 633.705 kb on + strand, within PGA1_c06200 at 633.713 kb on - strand, within PGA1_c06200 at 633.731 kb on + strand, within PGA1_c06200 at 633.830 kb on - strand, within PGA1_c06200 at 633.857 kb on + strand, within PGA1_c06200 at 633.880 kb on + strand, within PGA1_c06200 at 633.890 kb on + strand, within PGA1_c06200 at 634.051 kb on - strand at 634.074 kb on + strand at 634.111 kb on - strand at 634.240 kb on + strand, within PGA1_c06210 at 634.414 kb on - strand, within PGA1_c06210 at 634.438 kb on + strand, within PGA1_c06210 at 634.456 kb on - strand, within PGA1_c06210 at 634.491 kb on - strand, within PGA1_c06210 at 634.583 kb on + strand, within PGA1_c06210 at 634.679 kb on - strand, within PGA1_c06210 at 634.733 kb on + strand, within PGA1_c06210 at 634.733 kb on + strand, within PGA1_c06210 at 634.779 kb on + strand at 634.834 kb on - strand at 634.853 kb on + strand at 634.853 kb on + strand at 634.861 kb on - strand at 634.935 kb on + strand at 634.974 kb on - strand at 634.976 kb on + strand at 634.976 kb on + strand at 635.018 kb on + strand at 635.034 kb on + strand at 635.198 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 633,103 - PGA1_c06190 0.41 -1.9 633,121 + PGA1_c06190 0.45 -2.5 633,137 + PGA1_c06190 0.48 -1.2 633,184 - PGA1_c06190 0.58 +2.5 633,201 + PGA1_c06190 0.61 +2.6 633,201 + PGA1_c06190 0.61 +3.0 633,201 + PGA1_c06190 0.61 -2.3 633,220 + PGA1_c06190 0.65 +0.3 633,220 + PGA1_c06190 0.65 +1.7 633,222 + PGA1_c06190 0.65 -0.9 633,228 - PGA1_c06190 0.67 -1.3 633,257 - PGA1_c06190 0.73 -2.7 633,444 + +1.5 633,476 + +2.8 633,484 - -0.2 633,565 - PGA1_c06200 0.17 -0.9 633,610 - PGA1_c06200 0.24 -0.8 633,695 - PGA1_c06200 0.38 -1.0 633,705 + PGA1_c06200 0.40 +3.1 633,713 - PGA1_c06200 0.41 +1.0 633,731 + PGA1_c06200 0.44 -1.5 633,830 - PGA1_c06200 0.59 +2.2 633,857 + PGA1_c06200 0.64 +2.5 633,880 + PGA1_c06200 0.67 +3.2 633,890 + PGA1_c06200 0.69 +2.4 634,051 - +2.3 634,074 + +0.0 634,111 - +1.6 634,240 + PGA1_c06210 0.20 -2.5 634,414 - PGA1_c06210 0.44 -1.5 634,438 + PGA1_c06210 0.47 +0.4 634,456 - PGA1_c06210 0.50 +2.7 634,491 - PGA1_c06210 0.55 -2.1 634,583 + PGA1_c06210 0.67 +2.6 634,679 - PGA1_c06210 0.81 -1.2 634,733 + PGA1_c06210 0.88 +3.8 634,733 + PGA1_c06210 0.88 +1.5 634,779 + -3.0 634,834 - -3.0 634,853 + -0.6 634,853 + +2.5 634,861 - +0.0 634,935 + -1.5 634,974 - +1.3 634,976 + +0.7 634,976 + -0.8 635,018 + -0.4 635,034 + +0.5 635,198 - -2.6
Or see this region's nucleotide sequence