Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c06120 and PGA1_c06130 are separated by 200 nucleotides PGA1_c06130 and PGA1_c06140 are separated by 19 nucleotides PGA1_c06140 and PGA1_c06150 are separated by 179 nucleotides
PGA1_c06120: PGA1_c06120 - Flp pilus assembly protein, ATPase CpaE, at 626,301 to 627,533
_c06120
PGA1_c06130: PGA1_c06130 - outer membrane protein OmpA-like protein, at 627,734 to 628,360
_c06130
PGA1_c06140: PGA1_c06140 - putative bacterial type II/III secretion system protein, at 628,380 to 629,834
_c06140
PGA1_c06150: PGA1_c06150 - Flp pilus assembly protein CpaB-like protein, at 630,014 to 630,874
_c06150
Position (kb) 628
629
630 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 627.420 kb on - strand at 627.455 kb on + strand at 627.463 kb on - strand at 627.470 kb on + strand at 627.470 kb on + strand at 627.470 kb on + strand at 627.478 kb on - strand at 627.504 kb on + strand at 627.536 kb on + strand at 627.544 kb on - strand at 627.551 kb on - strand at 627.567 kb on - strand at 627.590 kb on + strand at 627.625 kb on - strand at 627.663 kb on - strand at 627.689 kb on - strand at 627.691 kb on + strand at 627.691 kb on + strand at 627.691 kb on + strand at 627.694 kb on - strand at 627.701 kb on + strand at 627.701 kb on + strand at 627.709 kb on - strand at 627.720 kb on - strand at 627.722 kb on + strand at 627.777 kb on + strand at 627.785 kb on - strand at 627.811 kb on + strand, within PGA1_c06130 at 627.982 kb on - strand, within PGA1_c06130 at 627.992 kb on + strand, within PGA1_c06130 at 628.000 kb on - strand, within PGA1_c06130 at 628.013 kb on + strand, within PGA1_c06130 at 628.136 kb on - strand, within PGA1_c06130 at 628.136 kb on - strand, within PGA1_c06130 at 628.157 kb on + strand, within PGA1_c06130 at 628.268 kb on + strand, within PGA1_c06130 at 628.268 kb on + strand, within PGA1_c06130 at 628.291 kb on - strand, within PGA1_c06130 at 628.291 kb on - strand, within PGA1_c06130 at 628.323 kb on + strand at 628.331 kb on - strand at 628.331 kb on - strand at 628.331 kb on - strand at 628.333 kb on + strand at 628.413 kb on + strand at 628.476 kb on - strand at 628.508 kb on - strand at 628.518 kb on + strand at 628.553 kb on - strand, within PGA1_c06140 at 628.613 kb on - strand, within PGA1_c06140 at 628.680 kb on + strand, within PGA1_c06140 at 628.680 kb on + strand, within PGA1_c06140 at 628.688 kb on - strand, within PGA1_c06140 at 628.701 kb on + strand, within PGA1_c06140 at 628.709 kb on - strand, within PGA1_c06140 at 628.735 kb on - strand, within PGA1_c06140 at 628.759 kb on + strand, within PGA1_c06140 at 628.843 kb on - strand, within PGA1_c06140 at 628.863 kb on + strand, within PGA1_c06140 at 628.890 kb on - strand, within PGA1_c06140 at 628.910 kb on - strand, within PGA1_c06140 at 628.926 kb on + strand, within PGA1_c06140 at 629.016 kb on - strand, within PGA1_c06140 at 629.053 kb on - strand, within PGA1_c06140 at 629.099 kb on - strand, within PGA1_c06140 at 629.128 kb on - strand, within PGA1_c06140 at 629.189 kb on + strand, within PGA1_c06140 at 629.214 kb on - strand, within PGA1_c06140 at 629.311 kb on - strand, within PGA1_c06140 at 629.353 kb on + strand, within PGA1_c06140 at 629.365 kb on + strand, within PGA1_c06140 at 629.378 kb on - strand, within PGA1_c06140 at 629.513 kb on + strand, within PGA1_c06140 at 629.513 kb on + strand, within PGA1_c06140 at 629.595 kb on - strand, within PGA1_c06140 at 629.668 kb on - strand, within PGA1_c06140 at 629.674 kb on + strand, within PGA1_c06140 at 629.762 kb on + strand at 629.809 kb on + strand at 629.845 kb on + strand at 629.883 kb on - strand at 629.911 kb on + strand at 629.914 kb on + strand at 629.943 kb on - strand at 630.036 kb on + strand at 630.046 kb on + strand at 630.054 kb on - strand at 630.062 kb on + strand at 630.070 kb on - strand at 630.080 kb on - strand at 630.080 kb on - strand at 630.105 kb on - strand, within PGA1_c06150 at 630.128 kb on - strand, within PGA1_c06150 at 630.134 kb on + strand, within PGA1_c06150 at 630.142 kb on - strand, within PGA1_c06150 at 630.246 kb on - strand, within PGA1_c06150 at 630.307 kb on + strand, within PGA1_c06150 at 630.322 kb on + strand, within PGA1_c06150 at 630.332 kb on + strand, within PGA1_c06150 at 630.337 kb on + strand, within PGA1_c06150 at 630.346 kb on - strand, within PGA1_c06150 at 630.413 kb on - strand, within PGA1_c06150 at 630.439 kb on + strand, within PGA1_c06150 at 630.439 kb on + strand, within PGA1_c06150 at 630.451 kb on + strand, within PGA1_c06150 at 630.485 kb on + strand, within PGA1_c06150 at 630.549 kb on - strand, within PGA1_c06150 at 630.566 kb on + strand, within PGA1_c06150 at 630.566 kb on + strand, within PGA1_c06150 at 630.571 kb on + strand, within PGA1_c06150 at 630.574 kb on - strand, within PGA1_c06150 at 630.574 kb on - strand, within PGA1_c06150 at 630.594 kb on - strand, within PGA1_c06150 at 630.674 kb on - strand, within PGA1_c06150 at 630.683 kb on + strand, within PGA1_c06150 at 630.683 kb on + strand, within PGA1_c06150 at 630.691 kb on - strand, within PGA1_c06150 at 630.707 kb on - strand, within PGA1_c06150 at 630.774 kb on + strand, within PGA1_c06150 at 630.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 627,420 - +1.4 627,455 + -3.2 627,463 - -0.5 627,470 + +1.1 627,470 + -1.3 627,470 + -2.0 627,478 - -1.9 627,504 + +2.5 627,536 + -0.6 627,544 - +2.1 627,551 - -0.5 627,567 - -1.5 627,590 + +1.1 627,625 - -0.7 627,663 - -2.8 627,689 - +0.8 627,691 + -2.3 627,691 + +0.9 627,691 + -1.0 627,694 - -2.8 627,701 + -1.4 627,701 + -1.1 627,709 - -1.0 627,720 - -1.3 627,722 + -1.8 627,777 + +0.2 627,785 - -1.3 627,811 + PGA1_c06130 0.12 +0.5 627,982 - PGA1_c06130 0.40 -2.1 627,992 + PGA1_c06130 0.41 -1.6 628,000 - PGA1_c06130 0.42 -0.4 628,013 + PGA1_c06130 0.44 -1.9 628,136 - PGA1_c06130 0.64 +1.5 628,136 - PGA1_c06130 0.64 +0.4 628,157 + PGA1_c06130 0.67 -0.8 628,268 + PGA1_c06130 0.85 +0.4 628,268 + PGA1_c06130 0.85 -2.6 628,291 - PGA1_c06130 0.89 +1.0 628,291 - PGA1_c06130 0.89 -2.1 628,323 + -0.7 628,331 - -1.7 628,331 - -0.7 628,331 - -1.2 628,333 + -1.5 628,413 + -2.2 628,476 - -1.6 628,508 - -2.3 628,518 + +0.9 628,553 - PGA1_c06140 0.12 -2.7 628,613 - PGA1_c06140 0.16 -1.2 628,680 + PGA1_c06140 0.21 -1.6 628,680 + PGA1_c06140 0.21 +2.4 628,688 - PGA1_c06140 0.21 -1.9 628,701 + PGA1_c06140 0.22 +1.3 628,709 - PGA1_c06140 0.23 -1.7 628,735 - PGA1_c06140 0.24 +0.7 628,759 + PGA1_c06140 0.26 -1.7 628,843 - PGA1_c06140 0.32 +2.5 628,863 + PGA1_c06140 0.33 -2.4 628,890 - PGA1_c06140 0.35 -1.8 628,910 - PGA1_c06140 0.36 -1.2 628,926 + PGA1_c06140 0.38 -1.2 629,016 - PGA1_c06140 0.44 +1.5 629,053 - PGA1_c06140 0.46 -1.9 629,099 - PGA1_c06140 0.49 -0.5 629,128 - PGA1_c06140 0.51 -1.8 629,189 + PGA1_c06140 0.56 -3.2 629,214 - PGA1_c06140 0.57 -2.0 629,311 - PGA1_c06140 0.64 -2.4 629,353 + PGA1_c06140 0.67 -1.5 629,365 + PGA1_c06140 0.68 -3.0 629,378 - PGA1_c06140 0.69 +1.2 629,513 + PGA1_c06140 0.78 -1.7 629,513 + PGA1_c06140 0.78 -2.4 629,595 - PGA1_c06140 0.84 -0.8 629,668 - PGA1_c06140 0.89 +1.1 629,674 + PGA1_c06140 0.89 -2.6 629,762 + +2.8 629,809 + +3.6 629,845 + -1.2 629,883 - -1.0 629,911 + -2.0 629,914 + -0.7 629,943 - -1.7 630,036 + +0.4 630,046 + -3.0 630,054 - +0.5 630,062 + -1.8 630,070 - +0.5 630,080 - -0.7 630,080 - -2.4 630,105 - PGA1_c06150 0.11 -1.7 630,128 - PGA1_c06150 0.13 -1.8 630,134 + PGA1_c06150 0.14 +1.9 630,142 - PGA1_c06150 0.15 +0.5 630,246 - PGA1_c06150 0.27 +1.5 630,307 + PGA1_c06150 0.34 -1.5 630,322 + PGA1_c06150 0.36 -1.7 630,332 + PGA1_c06150 0.37 -2.4 630,337 + PGA1_c06150 0.38 +1.0 630,346 - PGA1_c06150 0.39 +2.3 630,413 - PGA1_c06150 0.46 -0.4 630,439 + PGA1_c06150 0.49 -2.4 630,439 + PGA1_c06150 0.49 -0.8 630,451 + PGA1_c06150 0.51 +1.2 630,485 + PGA1_c06150 0.55 +1.1 630,549 - PGA1_c06150 0.62 -1.8 630,566 + PGA1_c06150 0.64 -1.9 630,566 + PGA1_c06150 0.64 -2.4 630,571 + PGA1_c06150 0.65 +1.1 630,574 - PGA1_c06150 0.65 +1.4 630,574 - PGA1_c06150 0.65 -2.0 630,594 - PGA1_c06150 0.67 +0.9 630,674 - PGA1_c06150 0.77 -2.5 630,683 + PGA1_c06150 0.78 +0.8 630,683 + PGA1_c06150 0.78 -2.4 630,691 - PGA1_c06150 0.79 -2.0 630,707 - PGA1_c06150 0.80 +1.1 630,774 + PGA1_c06150 0.88 -1.3 630,830 - +1.9
Or see this region's nucleotide sequence