Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05710 and PGA1_c05720 are separated by 193 nucleotides PGA1_c05720 and PGA1_c05730 are separated by 216 nucleotides PGA1_c05730 and PGA1_c05740 are separated by 228 nucleotides
PGA1_c05710: PGA1_c05710 - isoleucyl-tRNA synthetase IleS, at 584,015 to 586,945
_c05710
PGA1_c05720: PGA1_c05720 - hypothetical protein, at 587,139 to 587,483
_c05720
PGA1_c05730: PGA1_c05730 - putative methylated-DNA--protein-cysteine methyltransferase, at 587,700 to 588,188
_c05730
PGA1_c05740: PGA1_c05740 - phosphatidylcholine synthase Pcs, at 588,417 to 589,112
_c05740
Position (kb) 587
588 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 587.103 kb on + strand at 587.108 kb on + strand at 587.231 kb on + strand, within PGA1_c05720 at 587.246 kb on + strand, within PGA1_c05720 at 587.259 kb on - strand, within PGA1_c05720 at 587.368 kb on + strand, within PGA1_c05720 at 587.384 kb on - strand, within PGA1_c05720 at 587.415 kb on + strand, within PGA1_c05720 at 587.423 kb on - strand, within PGA1_c05720 at 587.478 kb on - strand at 587.548 kb on + strand at 587.550 kb on + strand at 587.556 kb on - strand at 587.556 kb on - strand at 587.556 kb on - strand at 587.556 kb on - strand at 587.575 kb on + strand at 587.637 kb on + strand at 587.645 kb on - strand at 587.645 kb on - strand at 587.666 kb on - strand at 587.671 kb on - strand at 587.690 kb on + strand at 587.773 kb on - strand, within PGA1_c05730 at 587.783 kb on - strand, within PGA1_c05730 at 588.049 kb on - strand, within PGA1_c05730 at 588.079 kb on + strand, within PGA1_c05730 at 588.203 kb on + strand at 588.216 kb on + strand at 588.224 kb on - strand at 588.234 kb on - strand at 588.391 kb on + strand at 588.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 587,103 + -1.9 587,108 + +0.5 587,231 + PGA1_c05720 0.27 -1.7 587,246 + PGA1_c05720 0.31 +0.3 587,259 - PGA1_c05720 0.35 -2.8 587,368 + PGA1_c05720 0.66 +1.6 587,384 - PGA1_c05720 0.71 -2.4 587,415 + PGA1_c05720 0.80 -2.8 587,423 - PGA1_c05720 0.82 +1.2 587,478 - +2.4 587,548 + +1.6 587,550 + +2.7 587,556 - +0.7 587,556 - +2.8 587,556 - +1.9 587,556 - -1.7 587,575 + -2.5 587,637 + +0.3 587,645 - +0.4 587,645 - -2.8 587,666 - -2.1 587,671 - +3.0 587,690 + -2.6 587,773 - PGA1_c05730 0.15 -0.1 587,783 - PGA1_c05730 0.17 +2.2 588,049 - PGA1_c05730 0.71 -0.8 588,079 + PGA1_c05730 0.78 -1.9 588,203 + -0.4 588,216 + +1.0 588,224 - +2.3 588,234 - +0.6 588,391 + -1.6 588,423 - +2.0
Or see this region's nucleotide sequence