Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05590 and PGA1_c05600 are separated by 261 nucleotides PGA1_c05600 and PGA1_c05610 overlap by 1 nucleotides
PGA1_c05590: PGA1_c05590 - acyl-CoA dehydrogenase-like protein, at 572,698 to 573,936
_c05590
PGA1_c05600: PGA1_c05600 - queuosine biosynthesis protein QueC, at 574,198 to 574,896
_c05600
PGA1_c05610: PGA1_c05610 - putative queuosine biosynthesis protein QueD, at 574,896 to 575,249
_c05610
Position (kb) 572
573
574 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 571.706 kb on + strand at 571.706 kb on + strand at 571.714 kb on - strand at 571.714 kb on - strand at 571.714 kb on - strand at 571.738 kb on + strand at 571.748 kb on + strand at 571.751 kb on - strand at 571.751 kb on - strand at 571.783 kb on + strand at 571.783 kb on + strand at 571.791 kb on - strand at 571.928 kb on + strand at 571.934 kb on - strand at 571.934 kb on - strand at 571.977 kb on - strand at 571.984 kb on + strand at 571.984 kb on + strand at 571.989 kb on + strand at 571.990 kb on + strand at 571.997 kb on - strand at 572.045 kb on + strand at 572.064 kb on - strand at 572.064 kb on - strand at 572.109 kb on - strand at 572.132 kb on - strand at 572.167 kb on - strand at 572.209 kb on - strand at 572.229 kb on + strand at 572.311 kb on + strand at 572.321 kb on - strand at 572.330 kb on - strand at 572.370 kb on + strand at 572.370 kb on + strand at 572.379 kb on - strand at 572.425 kb on - strand at 572.425 kb on - strand at 572.463 kb on + strand at 572.465 kb on + strand at 572.505 kb on + strand at 572.517 kb on - strand at 572.600 kb on - strand at 572.630 kb on + strand at 572.630 kb on + strand at 572.709 kb on - strand at 572.737 kb on + strand at 572.737 kb on + strand at 572.737 kb on + strand at 572.737 kb on + strand at 572.737 kb on + strand at 572.874 kb on + strand, within PGA1_c05590 at 572.880 kb on + strand, within PGA1_c05590 at 572.890 kb on - strand, within PGA1_c05590 at 572.931 kb on + strand, within PGA1_c05590 at 572.987 kb on + strand, within PGA1_c05590 at 572.995 kb on + strand, within PGA1_c05590 at 572.995 kb on - strand, within PGA1_c05590 at 572.995 kb on - strand, within PGA1_c05590 at 572.995 kb on - strand, within PGA1_c05590 at 573.003 kb on - strand, within PGA1_c05590 at 573.018 kb on - strand, within PGA1_c05590 at 573.043 kb on + strand, within PGA1_c05590 at 573.058 kb on - strand, within PGA1_c05590 at 573.088 kb on - strand, within PGA1_c05590 at 573.094 kb on + strand, within PGA1_c05590 at 573.160 kb on + strand, within PGA1_c05590 at 573.160 kb on + strand, within PGA1_c05590 at 573.181 kb on + strand, within PGA1_c05590 at 573.247 kb on + strand, within PGA1_c05590 at 573.361 kb on - strand, within PGA1_c05590 at 573.389 kb on + strand, within PGA1_c05590 at 573.390 kb on - strand, within PGA1_c05590 at 573.407 kb on + strand, within PGA1_c05590 at 573.407 kb on + strand, within PGA1_c05590 at 573.415 kb on - strand, within PGA1_c05590 at 573.430 kb on + strand, within PGA1_c05590 at 573.430 kb on + strand, within PGA1_c05590 at 573.430 kb on + strand, within PGA1_c05590 at 573.430 kb on + strand, within PGA1_c05590 at 573.482 kb on - strand, within PGA1_c05590 at 573.487 kb on - strand, within PGA1_c05590 at 573.525 kb on + strand, within PGA1_c05590 at 573.544 kb on - strand, within PGA1_c05590 at 573.662 kb on + strand, within PGA1_c05590 at 573.704 kb on + strand, within PGA1_c05590 at 573.706 kb on + strand, within PGA1_c05590 at 573.720 kb on - strand, within PGA1_c05590 at 573.726 kb on + strand, within PGA1_c05590 at 573.734 kb on - strand, within PGA1_c05590 at 573.734 kb on - strand, within PGA1_c05590 at 573.739 kb on + strand, within PGA1_c05590 at 573.760 kb on - strand, within PGA1_c05590 at 573.783 kb on - strand, within PGA1_c05590 at 573.825 kb on + strand at 573.825 kb on + strand at 573.833 kb on - strand at 573.833 kb on - strand at 573.833 kb on - strand at 573.834 kb on - strand at 573.864 kb on + strand at 573.879 kb on + strand at 573.918 kb on - strand at 573.999 kb on + strand at 573.999 kb on + strand at 573.999 kb on + strand at 574.007 kb on - strand at 574.025 kb on - strand at 574.080 kb on + strand at 574.080 kb on + strand at 574.088 kb on - strand at 574.088 kb on - strand at 574.088 kb on - strand at 574.088 kb on - strand at 574.093 kb on - strand at 574.094 kb on - strand at 574.094 kb on - strand at 574.135 kb on - strand at 574.152 kb on + strand at 574.153 kb on - strand at 574.215 kb on - strand at 574.215 kb on - strand at 574.272 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.357 kb on - strand, within PGA1_c05600 at 574.448 kb on + strand, within PGA1_c05600 at 574.451 kb on - strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.474 kb on + strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.533 kb on + strand, within PGA1_c05600 at 574.533 kb on + strand, within PGA1_c05600 at 574.566 kb on - strand, within PGA1_c05600 at 574.582 kb on + strand, within PGA1_c05600 at 574.600 kb on + strand, within PGA1_c05600 at 574.604 kb on + strand, within PGA1_c05600 at 574.657 kb on - strand, within PGA1_c05600 at 574.672 kb on + strand, within PGA1_c05600 at 574.693 kb on + strand, within PGA1_c05600 at 574.760 kb on - strand, within PGA1_c05600 at 574.768 kb on + strand, within PGA1_c05600 at 574.803 kb on + strand, within PGA1_c05600 at 574.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 571,706 + +1.6 571,706 + -1.3 571,714 - -3.6 571,714 - +2.1 571,714 - -2.7 571,738 + -0.6 571,748 + +2.4 571,751 - -1.9 571,751 - -0.8 571,783 + -1.9 571,783 + -2.2 571,791 - +0.9 571,928 + -0.8 571,934 - -2.7 571,934 - +1.7 571,977 - +1.0 571,984 + -1.1 571,984 + -1.1 571,989 + +0.7 571,990 + +2.8 571,997 - +1.8 572,045 + -1.5 572,064 - +2.2 572,064 - +0.9 572,109 - -2.4 572,132 - -1.4 572,167 - -2.9 572,209 - -1.6 572,229 + -2.6 572,311 + +1.7 572,321 - -1.2 572,330 - +1.5 572,370 + +2.2 572,370 + -1.6 572,379 - +2.5 572,425 - -1.0 572,425 - -3.1 572,463 + -0.6 572,465 + +2.0 572,505 + +1.5 572,517 - +2.1 572,600 - -2.2 572,630 + -2.6 572,630 + -1.7 572,709 - +2.9 572,737 + -1.3 572,737 + -1.5 572,737 + +1.3 572,737 + +1.0 572,737 + -1.7 572,874 + PGA1_c05590 0.14 +2.8 572,880 + PGA1_c05590 0.15 -1.8 572,890 - PGA1_c05590 0.15 +1.4 572,931 + PGA1_c05590 0.19 -1.8 572,987 + PGA1_c05590 0.23 -1.4 572,995 + PGA1_c05590 0.24 +2.3 572,995 - PGA1_c05590 0.24 +3.3 572,995 - PGA1_c05590 0.24 -0.7 572,995 - PGA1_c05590 0.24 -2.8 573,003 - PGA1_c05590 0.25 +2.5 573,018 - PGA1_c05590 0.26 -1.1 573,043 + PGA1_c05590 0.28 +3.0 573,058 - PGA1_c05590 0.29 -1.9 573,088 - PGA1_c05590 0.31 -2.6 573,094 + PGA1_c05590 0.32 +3.2 573,160 + PGA1_c05590 0.37 -1.8 573,160 + PGA1_c05590 0.37 -0.1 573,181 + PGA1_c05590 0.39 -1.0 573,247 + PGA1_c05590 0.44 -0.4 573,361 - PGA1_c05590 0.54 +2.1 573,389 + PGA1_c05590 0.56 -1.4 573,390 - PGA1_c05590 0.56 -0.5 573,407 + PGA1_c05590 0.57 -2.5 573,407 + PGA1_c05590 0.57 -1.8 573,415 - PGA1_c05590 0.58 +0.6 573,430 + PGA1_c05590 0.59 +3.0 573,430 + PGA1_c05590 0.59 -1.2 573,430 + PGA1_c05590 0.59 -0.5 573,430 + PGA1_c05590 0.59 -0.7 573,482 - PGA1_c05590 0.63 -1.8 573,487 - PGA1_c05590 0.64 -1.3 573,525 + PGA1_c05590 0.67 -2.1 573,544 - PGA1_c05590 0.68 +4.7 573,662 + PGA1_c05590 0.78 -1.0 573,704 + PGA1_c05590 0.81 +3.0 573,706 + PGA1_c05590 0.81 +1.7 573,720 - PGA1_c05590 0.82 +0.6 573,726 + PGA1_c05590 0.83 -1.7 573,734 - PGA1_c05590 0.84 -2.6 573,734 - PGA1_c05590 0.84 +2.1 573,739 + PGA1_c05590 0.84 +2.6 573,760 - PGA1_c05590 0.86 -1.7 573,783 - PGA1_c05590 0.88 +1.9 573,825 + +3.7 573,825 + -0.3 573,833 - +3.4 573,833 - -2.3 573,833 - -0.3 573,834 - -2.7 573,864 + -0.2 573,879 + -1.2 573,918 - +3.7 573,999 + +0.9 573,999 + +3.5 573,999 + -1.4 574,007 - +0.7 574,025 - -1.5 574,080 + +2.2 574,080 + +2.2 574,088 - -0.3 574,088 - +1.9 574,088 - +0.4 574,088 - -1.0 574,093 - -1.1 574,094 - -1.4 574,094 - -2.6 574,135 - -2.1 574,152 + +3.2 574,153 - +0.2 574,215 - -1.6 574,215 - +0.3 574,272 + PGA1_c05600 0.11 -0.8 574,295 + PGA1_c05600 0.14 +1.2 574,295 + PGA1_c05600 0.14 -1.5 574,295 + PGA1_c05600 0.14 -1.8 574,357 - PGA1_c05600 0.23 +1.6 574,448 + PGA1_c05600 0.36 +2.6 574,451 - PGA1_c05600 0.36 +0.6 574,467 + PGA1_c05600 0.38 +2.5 574,467 + PGA1_c05600 0.38 -0.7 574,467 + PGA1_c05600 0.38 +1.8 574,474 + PGA1_c05600 0.39 -2.6 574,475 - PGA1_c05600 0.40 -0.7 574,475 - PGA1_c05600 0.40 -1.8 574,475 - PGA1_c05600 0.40 -1.6 574,475 - PGA1_c05600 0.40 -0.4 574,533 + PGA1_c05600 0.48 +2.5 574,533 + PGA1_c05600 0.48 +0.0 574,566 - PGA1_c05600 0.53 -1.3 574,582 + PGA1_c05600 0.55 +1.6 574,600 + PGA1_c05600 0.58 -2.3 574,604 + PGA1_c05600 0.58 +2.1 574,657 - PGA1_c05600 0.66 -2.1 574,672 + PGA1_c05600 0.68 +2.0 574,693 + PGA1_c05600 0.71 -2.7 574,760 - PGA1_c05600 0.80 -1.6 574,768 + PGA1_c05600 0.82 +2.0 574,803 + PGA1_c05600 0.87 +0.7 574,852 + -2.8
Or see this region's nucleotide sequence