Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05530 and PGA1_c05540 are separated by 25 nucleotides PGA1_c05540 and PGA1_c05550 are separated by 342 nucleotides
PGA1_c05530: PGA1_c05530 - putative allantoin catabolism protein, at 564,946 to 565,938
_c05530
PGA1_c05540: PGA1_c05540 - transcriptional regulator, IclR family, at 565,964 to 566,782
_c05540
PGA1_c05550: PGA1_c05550 - glutamine synthase-like protein, at 567,125 to 568,576
_c05550
Position (kb) 565
566
567 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 564.974 kb on - strand at 565.007 kb on + strand at 565.153 kb on - strand, within PGA1_c05530 at 565.180 kb on - strand, within PGA1_c05530 at 565.186 kb on + strand, within PGA1_c05530 at 565.396 kb on + strand, within PGA1_c05530 at 565.437 kb on + strand, within PGA1_c05530 at 565.442 kb on + strand, within PGA1_c05530 at 565.449 kb on + strand, within PGA1_c05530 at 565.754 kb on - strand, within PGA1_c05530 at 565.762 kb on - strand, within PGA1_c05530 at 565.821 kb on - strand, within PGA1_c05530 at 565.828 kb on + strand, within PGA1_c05530 at 565.880 kb on + strand at 565.957 kb on + strand at 565.962 kb on + strand at 565.962 kb on + strand at 565.962 kb on + strand at 565.970 kb on - strand at 565.970 kb on - strand at 565.970 kb on - strand at 565.981 kb on - strand at 566.022 kb on + strand at 566.040 kb on - strand at 566.042 kb on - strand at 566.050 kb on + strand, within PGA1_c05540 at 566.075 kb on + strand, within PGA1_c05540 at 566.126 kb on + strand, within PGA1_c05540 at 566.146 kb on + strand, within PGA1_c05540 at 566.156 kb on - strand, within PGA1_c05540 at 566.158 kb on + strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.166 kb on - strand, within PGA1_c05540 at 566.173 kb on - strand, within PGA1_c05540 at 566.181 kb on + strand, within PGA1_c05540 at 566.238 kb on + strand, within PGA1_c05540 at 566.296 kb on + strand, within PGA1_c05540 at 566.312 kb on + strand, within PGA1_c05540 at 566.312 kb on + strand, within PGA1_c05540 at 566.313 kb on + strand, within PGA1_c05540 at 566.400 kb on - strand, within PGA1_c05540 at 566.410 kb on - strand, within PGA1_c05540 at 566.434 kb on + strand, within PGA1_c05540 at 566.444 kb on - strand, within PGA1_c05540 at 566.456 kb on + strand, within PGA1_c05540 at 566.473 kb on - strand, within PGA1_c05540 at 566.513 kb on - strand, within PGA1_c05540 at 566.515 kb on + strand, within PGA1_c05540 at 566.532 kb on - strand, within PGA1_c05540 at 566.550 kb on - strand, within PGA1_c05540 at 566.606 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.612 kb on + strand, within PGA1_c05540 at 566.615 kb on - strand, within PGA1_c05540 at 566.615 kb on - strand, within PGA1_c05540 at 566.620 kb on - strand, within PGA1_c05540 at 566.644 kb on + strand, within PGA1_c05540 at 566.650 kb on + strand, within PGA1_c05540 at 566.675 kb on + strand, within PGA1_c05540 at 566.770 kb on - strand at 566.799 kb on + strand at 566.800 kb on + strand at 566.800 kb on + strand at 566.808 kb on - strand at 566.830 kb on + strand at 566.831 kb on + strand at 566.831 kb on + strand at 566.831 kb on + strand at 566.831 kb on + strand at 566.882 kb on + strand at 566.882 kb on + strand at 566.890 kb on - strand at 566.895 kb on - strand at 567.052 kb on - strand at 567.057 kb on - strand at 567.059 kb on - strand at 567.063 kb on + strand at 567.065 kb on + strand at 567.142 kb on - strand at 567.176 kb on + strand at 567.189 kb on - strand at 567.192 kb on + strand at 567.214 kb on + strand at 567.214 kb on + strand at 567.222 kb on - strand at 567.229 kb on + strand at 567.237 kb on + strand at 567.237 kb on - strand at 567.285 kb on - strand, within PGA1_c05550 at 567.299 kb on + strand, within PGA1_c05550 at 567.302 kb on - strand, within PGA1_c05550 at 567.354 kb on - strand, within PGA1_c05550 at 567.414 kb on + strand, within PGA1_c05550 at 567.475 kb on + strand, within PGA1_c05550 at 567.475 kb on + strand, within PGA1_c05550 at 567.475 kb on + strand, within PGA1_c05550 at 567.488 kb on + strand, within PGA1_c05550 at 567.536 kb on - strand, within PGA1_c05550 at 567.565 kb on - strand, within PGA1_c05550 at 567.653 kb on + strand, within PGA1_c05550 at 567.653 kb on + strand, within PGA1_c05550 at 567.656 kb on - strand, within PGA1_c05550 at 567.699 kb on + strand, within PGA1_c05550 at 567.710 kb on + strand, within PGA1_c05550 at 567.739 kb on + strand, within PGA1_c05550 at 567.751 kb on + strand, within PGA1_c05550
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 564,974 - -2.7 565,007 + +0.3 565,153 - PGA1_c05530 0.21 +2.5 565,180 - PGA1_c05530 0.24 -2.4 565,186 + PGA1_c05530 0.24 -1.1 565,396 + PGA1_c05530 0.45 -0.9 565,437 + PGA1_c05530 0.49 -2.2 565,442 + PGA1_c05530 0.50 -3.1 565,449 + PGA1_c05530 0.51 -0.9 565,754 - PGA1_c05530 0.81 -0.8 565,762 - PGA1_c05530 0.82 -1.1 565,821 - PGA1_c05530 0.88 -1.2 565,828 + PGA1_c05530 0.89 +0.9 565,880 + -2.9 565,957 + -1.8 565,962 + -1.3 565,962 + -3.1 565,962 + +1.0 565,970 - +0.3 565,970 - +2.8 565,970 - +0.6 565,981 - +1.7 566,022 + -0.4 566,040 - +2.6 566,042 - -1.6 566,050 + PGA1_c05540 0.11 -1.2 566,075 + PGA1_c05540 0.14 -0.1 566,126 + PGA1_c05540 0.20 -2.1 566,146 + PGA1_c05540 0.22 +1.4 566,156 - PGA1_c05540 0.23 +1.0 566,158 + PGA1_c05540 0.24 +1.8 566,166 - PGA1_c05540 0.25 -1.4 566,166 - PGA1_c05540 0.25 -2.7 566,166 - PGA1_c05540 0.25 +3.2 566,166 - PGA1_c05540 0.25 -1.2 566,166 - PGA1_c05540 0.25 -0.5 566,166 - PGA1_c05540 0.25 +1.7 566,173 - PGA1_c05540 0.26 +2.3 566,181 + PGA1_c05540 0.26 -1.4 566,238 + PGA1_c05540 0.33 -2.3 566,296 + PGA1_c05540 0.41 +1.7 566,312 + PGA1_c05540 0.42 -2.5 566,312 + PGA1_c05540 0.42 +1.6 566,313 + PGA1_c05540 0.43 -1.0 566,400 - PGA1_c05540 0.53 -3.0 566,410 - PGA1_c05540 0.54 +1.9 566,434 + PGA1_c05540 0.57 +0.3 566,444 - PGA1_c05540 0.59 -2.2 566,456 + PGA1_c05540 0.60 -0.3 566,473 - PGA1_c05540 0.62 +1.5 566,513 - PGA1_c05540 0.67 -0.3 566,515 + PGA1_c05540 0.67 -2.5 566,532 - PGA1_c05540 0.69 -1.0 566,550 - PGA1_c05540 0.72 -0.9 566,606 + PGA1_c05540 0.78 +0.9 566,612 + PGA1_c05540 0.79 -1.2 566,612 + PGA1_c05540 0.79 +3.7 566,612 + PGA1_c05540 0.79 -2.2 566,612 + PGA1_c05540 0.79 -1.2 566,612 + PGA1_c05540 0.79 +1.7 566,612 + PGA1_c05540 0.79 -0.1 566,612 + PGA1_c05540 0.79 +1.6 566,612 + PGA1_c05540 0.79 +0.4 566,615 - PGA1_c05540 0.79 -2.5 566,615 - PGA1_c05540 0.79 +0.3 566,620 - PGA1_c05540 0.80 +0.8 566,644 + PGA1_c05540 0.83 -2.9 566,650 + PGA1_c05540 0.84 -2.8 566,675 + PGA1_c05540 0.87 +1.9 566,770 - -2.3 566,799 + +1.5 566,800 + -0.3 566,800 + -1.7 566,808 - +1.5 566,830 + +0.3 566,831 + +3.4 566,831 + -1.3 566,831 + +2.9 566,831 + +0.2 566,882 + -0.8 566,882 + +1.9 566,890 - -0.7 566,895 - -1.0 567,052 - +1.4 567,057 - +1.9 567,059 - +0.6 567,063 + +1.7 567,065 + -2.0 567,142 - +2.2 567,176 + +2.6 567,189 - -0.8 567,192 + +3.9 567,214 + -1.8 567,214 + +0.5 567,222 - -1.3 567,229 + -0.7 567,237 + +2.3 567,237 - -1.0 567,285 - PGA1_c05550 0.11 -2.0 567,299 + PGA1_c05550 0.12 +3.2 567,302 - PGA1_c05550 0.12 +1.8 567,354 - PGA1_c05550 0.16 +0.6 567,414 + PGA1_c05550 0.20 +1.4 567,475 + PGA1_c05550 0.24 -3.3 567,475 + PGA1_c05550 0.24 -0.3 567,475 + PGA1_c05550 0.24 +1.3 567,488 + PGA1_c05550 0.25 -0.3 567,536 - PGA1_c05550 0.28 +0.6 567,565 - PGA1_c05550 0.30 +0.8 567,653 + PGA1_c05550 0.36 -2.5 567,653 + PGA1_c05550 0.36 +1.4 567,656 - PGA1_c05550 0.37 +1.0 567,699 + PGA1_c05550 0.40 +0.8 567,710 + PGA1_c05550 0.40 -2.5 567,739 + PGA1_c05550 0.42 +1.8 567,751 + PGA1_c05550 0.43 -2.0
Or see this region's nucleotide sequence