Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05480 and PGA1_c05490 are separated by 43 nucleotides PGA1_c05490 and PGA1_c05500 are separated by 135 nucleotides PGA1_c05500 and PGA1_c05510 are separated by 105 nucleotides
PGA1_c05480: PGA1_c05480 - OHCU decarboxylase domain-containing protein, at 559,611 to 561,026
_c05480
PGA1_c05490: PGA1_c05490 - ureidoglycolate hydrolase AllA, at 561,070 to 561,555
_c05490
PGA1_c05500: PGA1_c05500 - putative purine permease, at 561,691 to 563,118
_c05500
PGA1_c05510: PGA1_c05510 - glyceraldehyde-3-phosphate dehydrogenase 1, at 563,224 to 564,285
_c05510
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 560.714 kb on - strand, within PGA1_c05480 at 560.757 kb on - strand, within PGA1_c05480 at 560.779 kb on - strand, within PGA1_c05480 at 560.786 kb on + strand, within PGA1_c05480 at 560.838 kb on - strand, within PGA1_c05480 at 560.923 kb on + strand at 560.923 kb on + strand at 560.923 kb on + strand at 560.923 kb on + strand at 560.923 kb on + strand at 560.931 kb on - strand at 560.931 kb on - strand at 560.931 kb on - strand at 561.023 kb on - strand at 561.025 kb on + strand at 561.028 kb on - strand at 561.038 kb on + strand at 561.046 kb on - strand at 561.106 kb on - strand at 561.107 kb on + strand at 561.168 kb on + strand, within PGA1_c05490 at 561.207 kb on - strand, within PGA1_c05490 at 561.237 kb on + strand, within PGA1_c05490 at 561.261 kb on + strand, within PGA1_c05490 at 561.284 kb on - strand, within PGA1_c05490 at 561.307 kb on + strand, within PGA1_c05490 at 561.334 kb on - strand, within PGA1_c05490 at 561.350 kb on + strand, within PGA1_c05490 at 561.355 kb on + strand, within PGA1_c05490 at 561.375 kb on - strand, within PGA1_c05490 at 561.416 kb on + strand, within PGA1_c05490 at 561.416 kb on + strand, within PGA1_c05490 at 561.434 kb on + strand, within PGA1_c05490 at 561.514 kb on + strand at 561.530 kb on + strand at 561.583 kb on - strand at 561.645 kb on - strand at 561.689 kb on - strand at 561.689 kb on - strand at 561.689 kb on - strand at 561.713 kb on + strand at 561.753 kb on + strand at 561.753 kb on + strand at 561.753 kb on + strand at 561.761 kb on - strand at 561.761 kb on - strand at 561.789 kb on + strand at 561.792 kb on - strand at 561.797 kb on - strand at 561.797 kb on - strand at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.861 kb on - strand, within PGA1_c05500 at 561.861 kb on - strand, within PGA1_c05500 at 561.914 kb on - strand, within PGA1_c05500 at 561.920 kb on + strand, within PGA1_c05500 at 561.976 kb on + strand, within PGA1_c05500 at 561.979 kb on - strand, within PGA1_c05500 at 561.984 kb on - strand, within PGA1_c05500 at 562.037 kb on - strand, within PGA1_c05500 at 562.056 kb on + strand, within PGA1_c05500 at 562.056 kb on + strand, within PGA1_c05500 at 562.056 kb on + strand, within PGA1_c05500 at 562.124 kb on - strand, within PGA1_c05500 at 562.169 kb on + strand, within PGA1_c05500 at 562.169 kb on + strand, within PGA1_c05500 at 562.179 kb on + strand, within PGA1_c05500 at 562.187 kb on - strand, within PGA1_c05500 at 562.256 kb on + strand, within PGA1_c05500 at 562.286 kb on + strand, within PGA1_c05500 at 562.286 kb on + strand, within PGA1_c05500 at 562.291 kb on + strand, within PGA1_c05500 at 562.383 kb on + strand, within PGA1_c05500 at 562.383 kb on + strand, within PGA1_c05500 at 562.391 kb on - strand, within PGA1_c05500 at 562.420 kb on + strand, within PGA1_c05500 at 562.427 kb on + strand, within PGA1_c05500 at 562.435 kb on - strand, within PGA1_c05500 at 562.481 kb on + strand, within PGA1_c05500 at 562.509 kb on - strand, within PGA1_c05500 at 562.641 kb on - strand, within PGA1_c05500 at 562.647 kb on + strand, within PGA1_c05500 at 562.647 kb on + strand, within PGA1_c05500 at 562.664 kb on + strand, within PGA1_c05500 at 562.683 kb on - strand, within PGA1_c05500 at 562.739 kb on - strand, within PGA1_c05500 at 562.755 kb on + strand, within PGA1_c05500 at 562.918 kb on - strand, within PGA1_c05500 at 562.945 kb on + strand, within PGA1_c05500 at 562.953 kb on - strand, within PGA1_c05500 at 562.956 kb on + strand, within PGA1_c05500 at 562.982 kb on + strand at 562.982 kb on + strand at 562.982 kb on + strand at 562.990 kb on - strand at 562.990 kb on - strand at 562.992 kb on + strand at 563.012 kb on + strand at 563.017 kb on + strand at 563.017 kb on + strand at 563.025 kb on - strand at 563.086 kb on + strand at 563.092 kb on + strand at 563.099 kb on - strand at 563.123 kb on + strand at 563.123 kb on + strand at 563.128 kb on + strand at 563.133 kb on + strand at 563.135 kb on - strand at 563.136 kb on - strand at 563.376 kb on + strand, within PGA1_c05510 at 563.376 kb on + strand, within PGA1_c05510 at 563.381 kb on + strand, within PGA1_c05510 at 563.384 kb on - strand, within PGA1_c05510 at 563.384 kb on - strand, within PGA1_c05510 at 563.410 kb on - strand, within PGA1_c05510 at 563.410 kb on - strand, within PGA1_c05510 at 563.488 kb on - strand, within PGA1_c05510 at 563.649 kb on + strand, within PGA1_c05510 at 563.734 kb on - strand, within PGA1_c05510 at 563.734 kb on - strand, within PGA1_c05510 at 563.735 kb on + strand, within PGA1_c05510 at 563.782 kb on - strand, within PGA1_c05510 at 563.831 kb on - strand, within PGA1_c05510 at 563.860 kb on + strand, within PGA1_c05510 at 563.904 kb on + strand, within PGA1_c05510 at 563.904 kb on + strand, within PGA1_c05510 at 563.958 kb on + strand, within PGA1_c05510 at 563.981 kb on - strand, within PGA1_c05510 at 563.981 kb on - strand, within PGA1_c05510 at 564.026 kb on - strand, within PGA1_c05510 at 564.026 kb on - strand, within PGA1_c05510 at 564.054 kb on + strand, within PGA1_c05510 at 564.062 kb on + strand, within PGA1_c05510 at 564.062 kb on - strand, within PGA1_c05510 at 564.102 kb on - strand, within PGA1_c05510
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 560,714 - PGA1_c05480 0.78 +2.1 560,757 - PGA1_c05480 0.81 -1.2 560,779 - PGA1_c05480 0.82 +3.0 560,786 + PGA1_c05480 0.83 +2.5 560,838 - PGA1_c05480 0.87 +0.7 560,923 + +1.5 560,923 + +1.6 560,923 + +2.1 560,923 + +2.5 560,923 + -2.6 560,931 - +2.0 560,931 - -0.7 560,931 - -2.4 561,023 - +0.1 561,025 + -1.8 561,028 - -0.8 561,038 + -0.5 561,046 - +1.2 561,106 - +2.0 561,107 + +1.2 561,168 + PGA1_c05490 0.20 +2.2 561,207 - PGA1_c05490 0.28 +1.9 561,237 + PGA1_c05490 0.34 +1.0 561,261 + PGA1_c05490 0.39 -1.8 561,284 - PGA1_c05490 0.44 +2.3 561,307 + PGA1_c05490 0.49 +2.3 561,334 - PGA1_c05490 0.54 +0.1 561,350 + PGA1_c05490 0.58 -2.0 561,355 + PGA1_c05490 0.59 +2.8 561,375 - PGA1_c05490 0.63 -0.7 561,416 + PGA1_c05490 0.71 -1.6 561,416 + PGA1_c05490 0.71 -2.5 561,434 + PGA1_c05490 0.75 -2.0 561,514 + -2.3 561,530 + -1.4 561,583 - +3.7 561,645 - +0.2 561,689 - +1.1 561,689 - +1.6 561,689 - -1.0 561,713 + -1.4 561,753 + +0.1 561,753 + -0.4 561,753 + -2.8 561,761 - -0.9 561,761 - -1.9 561,789 + +3.3 561,792 - -2.9 561,797 - +2.6 561,797 - +0.5 561,853 + PGA1_c05500 0.11 +2.0 561,853 + PGA1_c05500 0.11 +2.3 561,853 + PGA1_c05500 0.11 +1.6 561,853 + PGA1_c05500 0.11 -3.4 561,853 + PGA1_c05500 0.11 +0.6 561,861 - PGA1_c05500 0.12 -1.4 561,861 - PGA1_c05500 0.12 +1.7 561,914 - PGA1_c05500 0.16 -1.6 561,920 + PGA1_c05500 0.16 -2.2 561,976 + PGA1_c05500 0.20 +2.5 561,979 - PGA1_c05500 0.20 +3.3 561,984 - PGA1_c05500 0.21 +3.7 562,037 - PGA1_c05500 0.24 +3.1 562,056 + PGA1_c05500 0.26 +1.9 562,056 + PGA1_c05500 0.26 +1.6 562,056 + PGA1_c05500 0.26 +3.2 562,124 - PGA1_c05500 0.30 -1.9 562,169 + PGA1_c05500 0.33 -2.4 562,169 + PGA1_c05500 0.33 +3.0 562,179 + PGA1_c05500 0.34 +0.5 562,187 - PGA1_c05500 0.35 -1.7 562,256 + PGA1_c05500 0.40 +1.0 562,286 + PGA1_c05500 0.42 +1.0 562,286 + PGA1_c05500 0.42 -2.3 562,291 + PGA1_c05500 0.42 +1.7 562,383 + PGA1_c05500 0.48 -0.4 562,383 + PGA1_c05500 0.48 -0.8 562,391 - PGA1_c05500 0.49 +1.1 562,420 + PGA1_c05500 0.51 +2.4 562,427 + PGA1_c05500 0.52 -1.3 562,435 - PGA1_c05500 0.52 -2.2 562,481 + PGA1_c05500 0.55 -0.3 562,509 - PGA1_c05500 0.57 -3.0 562,641 - PGA1_c05500 0.67 +4.6 562,647 + PGA1_c05500 0.67 +2.3 562,647 + PGA1_c05500 0.67 +1.2 562,664 + PGA1_c05500 0.68 +1.6 562,683 - PGA1_c05500 0.69 +2.4 562,739 - PGA1_c05500 0.73 -0.5 562,755 + PGA1_c05500 0.75 -2.2 562,918 - PGA1_c05500 0.86 -1.1 562,945 + PGA1_c05500 0.88 +2.9 562,953 - PGA1_c05500 0.88 +1.6 562,956 + PGA1_c05500 0.89 +2.1 562,982 + +3.0 562,982 + -1.2 562,982 + -1.8 562,990 - +1.8 562,990 - +0.3 562,992 + -1.7 563,012 + +2.3 563,017 + -1.3 563,017 + -2.1 563,025 - +1.2 563,086 + -1.4 563,092 + +3.1 563,099 - -2.1 563,123 + -1.5 563,123 + -2.2 563,128 + +2.3 563,133 + +1.8 563,135 - -2.6 563,136 - +0.1 563,376 + PGA1_c05510 0.14 -1.9 563,376 + PGA1_c05510 0.14 -1.9 563,381 + PGA1_c05510 0.15 +1.6 563,384 - PGA1_c05510 0.15 +2.3 563,384 - PGA1_c05510 0.15 +1.5 563,410 - PGA1_c05510 0.18 +3.6 563,410 - PGA1_c05510 0.18 -3.3 563,488 - PGA1_c05510 0.25 -0.1 563,649 + PGA1_c05510 0.40 +2.8 563,734 - PGA1_c05510 0.48 -0.8 563,734 - PGA1_c05510 0.48 +2.0 563,735 + PGA1_c05510 0.48 +2.0 563,782 - PGA1_c05510 0.53 -1.0 563,831 - PGA1_c05510 0.57 +0.8 563,860 + PGA1_c05510 0.60 -1.6 563,904 + PGA1_c05510 0.64 -2.4 563,904 + PGA1_c05510 0.64 +0.8 563,958 + PGA1_c05510 0.69 -1.6 563,981 - PGA1_c05510 0.71 +0.5 563,981 - PGA1_c05510 0.71 +2.8 564,026 - PGA1_c05510 0.76 -2.4 564,026 - PGA1_c05510 0.76 -0.7 564,054 + PGA1_c05510 0.78 +1.8 564,062 + PGA1_c05510 0.79 +0.1 564,062 - PGA1_c05510 0.79 -2.6 564,102 - PGA1_c05510 0.83 -2.9
Or see this region's nucleotide sequence