Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05380 and PGA1_c05390 are separated by 161 nucleotides PGA1_c05390 and PGA1_c05400 are separated by 19 nucleotides PGA1_c05400 and PGA1_c05410 overlap by 1 nucleotides
PGA1_c05380: PGA1_c05380 - hypothetical protein, at 545,206 to 546,669
_c05380
PGA1_c05390: PGA1_c05390 - glycosyl transferase, at 546,831 to 547,583
_c05390
PGA1_c05400: PGA1_c05400 - putative glycosyl transferase, family 2, at 547,603 to 548,586
_c05400
PGA1_c05410: PGA1_c05410 - hypothetical protein, at 548,586 to 549,695
_c05410
Position (kb)
547
548
549 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 546.612 kb on + strand at 546.612 kb on + strand at 546.612 kb on + strand at 546.612 kb on + strand at 546.620 kb on - strand at 546.620 kb on - strand at 546.642 kb on + strand at 546.642 kb on + strand at 546.649 kb on - strand at 546.650 kb on - strand at 546.652 kb on + strand at 546.686 kb on - strand at 546.795 kb on + strand at 546.838 kb on - strand at 546.861 kb on + strand at 546.861 kb on + strand at 546.957 kb on - strand, within PGA1_c05390 at 546.995 kb on + strand, within PGA1_c05390 at 546.995 kb on + strand, within PGA1_c05390 at 546.995 kb on + strand, within PGA1_c05390 at 547.003 kb on - strand, within PGA1_c05390 at 547.056 kb on - strand, within PGA1_c05390 at 547.136 kb on + strand, within PGA1_c05390 at 547.210 kb on + strand, within PGA1_c05390 at 547.219 kb on - strand, within PGA1_c05390 at 547.312 kb on + strand, within PGA1_c05390 at 547.407 kb on + strand, within PGA1_c05390 at 547.415 kb on - strand, within PGA1_c05390 at 547.415 kb on - strand, within PGA1_c05390 at 547.415 kb on - strand, within PGA1_c05390 at 547.512 kb on - strand at 547.520 kb on - strand at 547.536 kb on - strand at 547.569 kb on - strand at 547.580 kb on - strand at 547.585 kb on - strand at 547.675 kb on - strand at 547.695 kb on - strand at 547.700 kb on - strand at 547.700 kb on - strand at 547.700 kb on - strand at 547.826 kb on + strand, within PGA1_c05400 at 547.826 kb on + strand, within PGA1_c05400 at 547.834 kb on - strand, within PGA1_c05400 at 547.834 kb on - strand, within PGA1_c05400 at 547.856 kb on + strand, within PGA1_c05400 at 547.882 kb on + strand, within PGA1_c05400 at 547.914 kb on - strand, within PGA1_c05400 at 547.925 kb on - strand, within PGA1_c05400 at 548.039 kb on - strand, within PGA1_c05400 at 548.077 kb on + strand, within PGA1_c05400 at 548.079 kb on + strand, within PGA1_c05400 at 548.079 kb on + strand, within PGA1_c05400 at 548.085 kb on - strand, within PGA1_c05400 at 548.085 kb on - strand, within PGA1_c05400 at 548.087 kb on - strand, within PGA1_c05400 at 548.087 kb on - strand, within PGA1_c05400 at 548.106 kb on - strand, within PGA1_c05400 at 548.119 kb on + strand, within PGA1_c05400 at 548.119 kb on + strand, within PGA1_c05400 at 548.234 kb on + strand, within PGA1_c05400 at 548.242 kb on - strand, within PGA1_c05400 at 548.242 kb on - strand, within PGA1_c05400 at 548.242 kb on - strand, within PGA1_c05400 at 548.242 kb on - strand, within PGA1_c05400 at 548.242 kb on - strand, within PGA1_c05400 at 548.329 kb on + strand, within PGA1_c05400 at 548.329 kb on + strand, within PGA1_c05400 at 548.332 kb on - strand, within PGA1_c05400 at 548.337 kb on - strand, within PGA1_c05400 at 548.337 kb on - strand, within PGA1_c05400 at 548.357 kb on + strand, within PGA1_c05400 at 548.357 kb on + strand, within PGA1_c05400 at 548.365 kb on - strand, within PGA1_c05400 at 548.380 kb on - strand, within PGA1_c05400 at 548.464 kb on + strand, within PGA1_c05400 at 548.515 kb on - strand at 548.547 kb on - strand at 548.567 kb on + strand at 548.567 kb on + strand at 548.648 kb on + strand at 548.671 kb on + strand at 548.778 kb on + strand, within PGA1_c05410 at 548.779 kb on + strand, within PGA1_c05410 at 548.787 kb on - strand, within PGA1_c05410 at 548.836 kb on - strand, within PGA1_c05410 at 548.836 kb on - strand, within PGA1_c05410 at 548.876 kb on + strand, within PGA1_c05410 at 548.944 kb on - strand, within PGA1_c05410 at 548.973 kb on - strand, within PGA1_c05410 at 548.989 kb on + strand, within PGA1_c05410 at 548.989 kb on + strand, within PGA1_c05410 at 549.059 kb on - strand, within PGA1_c05410 at 549.059 kb on - strand, within PGA1_c05410 at 549.069 kb on - strand, within PGA1_c05410 at 549.108 kb on - strand, within PGA1_c05410 at 549.108 kb on - strand, within PGA1_c05410 at 549.108 kb on - strand, within PGA1_c05410 at 549.113 kb on - strand, within PGA1_c05410 at 549.170 kb on + strand, within PGA1_c05410 at 549.185 kb on + strand, within PGA1_c05410 at 549.212 kb on + strand, within PGA1_c05410 at 549.216 kb on + strand, within PGA1_c05410 at 549.218 kb on + strand, within PGA1_c05410 at 549.224 kb on - strand, within PGA1_c05410 at 549.291 kb on - strand, within PGA1_c05410 at 549.333 kb on + strand, within PGA1_c05410 at 549.336 kb on - strand, within PGA1_c05410 at 549.341 kb on - strand, within PGA1_c05410 at 549.351 kb on - strand, within PGA1_c05410 at 549.439 kb on - strand, within PGA1_c05410 at 549.444 kb on + strand, within PGA1_c05410 at 549.464 kb on - strand, within PGA1_c05410 at 549.469 kb on - strand, within PGA1_c05410 at 549.543 kb on - strand, within PGA1_c05410 at 549.584 kb on - strand, within PGA1_c05410
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 546,612 + -1.8 546,612 + +1.1 546,612 + -2.0 546,612 + +1.4 546,620 - +0.8 546,620 - -2.3 546,642 + -1.0 546,642 + -1.4 546,649 - +3.7 546,650 - -1.5 546,652 + -0.9 546,686 - +1.4 546,795 + -0.8 546,838 - +1.8 546,861 + +2.0 546,861 + -2.9 546,957 - PGA1_c05390 0.17 +1.6 546,995 + PGA1_c05390 0.22 -2.2 546,995 + PGA1_c05390 0.22 -1.6 546,995 + PGA1_c05390 0.22 -1.2 547,003 - PGA1_c05390 0.23 +2.7 547,056 - PGA1_c05390 0.30 +2.2 547,136 + PGA1_c05390 0.41 +0.7 547,210 + PGA1_c05390 0.50 +0.9 547,219 - PGA1_c05390 0.52 -1.2 547,312 + PGA1_c05390 0.64 -2.0 547,407 + PGA1_c05390 0.76 -1.4 547,415 - PGA1_c05390 0.78 +2.6 547,415 - PGA1_c05390 0.78 -2.9 547,415 - PGA1_c05390 0.78 +2.0 547,512 - +4.2 547,520 - +3.6 547,536 - -1.4 547,569 - -0.7 547,580 - +0.9 547,585 - -1.3 547,675 - +3.4 547,695 - -2.0 547,700 - +1.5 547,700 - +2.1 547,700 - +2.2 547,826 + PGA1_c05400 0.23 +1.3 547,826 + PGA1_c05400 0.23 +2.2 547,834 - PGA1_c05400 0.23 +1.9 547,834 - PGA1_c05400 0.23 -1.7 547,856 + PGA1_c05400 0.26 +1.4 547,882 + PGA1_c05400 0.28 -1.6 547,914 - PGA1_c05400 0.32 -1.7 547,925 - PGA1_c05400 0.33 +3.5 548,039 - PGA1_c05400 0.44 -1.4 548,077 + PGA1_c05400 0.48 -2.0 548,079 + PGA1_c05400 0.48 +3.4 548,079 + PGA1_c05400 0.48 +1.9 548,085 - PGA1_c05400 0.49 -1.5 548,085 - PGA1_c05400 0.49 +1.5 548,087 - PGA1_c05400 0.49 +1.0 548,087 - PGA1_c05400 0.49 +3.2 548,106 - PGA1_c05400 0.51 +2.0 548,119 + PGA1_c05400 0.52 +3.6 548,119 + PGA1_c05400 0.52 -1.8 548,234 + PGA1_c05400 0.64 -0.7 548,242 - PGA1_c05400 0.65 +2.9 548,242 - PGA1_c05400 0.65 +4.3 548,242 - PGA1_c05400 0.65 -0.4 548,242 - PGA1_c05400 0.65 -1.2 548,242 - PGA1_c05400 0.65 +2.7 548,329 + PGA1_c05400 0.74 +3.1 548,329 + PGA1_c05400 0.74 +2.4 548,332 - PGA1_c05400 0.74 -1.9 548,337 - PGA1_c05400 0.75 +1.0 548,337 - PGA1_c05400 0.75 +2.0 548,357 + PGA1_c05400 0.77 +4.0 548,357 + PGA1_c05400 0.77 -2.9 548,365 - PGA1_c05400 0.77 -2.0 548,380 - PGA1_c05400 0.79 -0.6 548,464 + PGA1_c05400 0.88 -1.7 548,515 - -0.8 548,547 - +0.2 548,567 + +1.8 548,567 + -0.3 548,648 + +3.7 548,671 + +2.3 548,778 + PGA1_c05410 0.17 -1.8 548,779 + PGA1_c05410 0.17 -2.0 548,787 - PGA1_c05410 0.18 -2.8 548,836 - PGA1_c05410 0.23 -1.5 548,836 - PGA1_c05410 0.23 -2.0 548,876 + PGA1_c05410 0.26 +2.6 548,944 - PGA1_c05410 0.32 +4.2 548,973 - PGA1_c05410 0.35 -0.5 548,989 + PGA1_c05410 0.36 +1.4 548,989 + PGA1_c05410 0.36 +2.9 549,059 - PGA1_c05410 0.43 +4.0 549,059 - PGA1_c05410 0.43 -1.1 549,069 - PGA1_c05410 0.44 +2.7 549,108 - PGA1_c05410 0.47 +1.3 549,108 - PGA1_c05410 0.47 +2.8 549,108 - PGA1_c05410 0.47 +1.9 549,113 - PGA1_c05410 0.47 +1.9 549,170 + PGA1_c05410 0.53 -1.1 549,185 + PGA1_c05410 0.54 +0.9 549,212 + PGA1_c05410 0.56 +2.0 549,216 + PGA1_c05410 0.57 -1.8 549,218 + PGA1_c05410 0.57 +3.6 549,224 - PGA1_c05410 0.57 +1.7 549,291 - PGA1_c05410 0.64 +2.8 549,333 + PGA1_c05410 0.67 -1.3 549,336 - PGA1_c05410 0.68 +3.1 549,341 - PGA1_c05410 0.68 +1.4 549,351 - PGA1_c05410 0.69 -1.9 549,439 - PGA1_c05410 0.77 +1.7 549,444 + PGA1_c05410 0.77 +2.2 549,464 - PGA1_c05410 0.79 +3.4 549,469 - PGA1_c05410 0.80 +0.4 549,543 - PGA1_c05410 0.86 +3.0 549,584 - PGA1_c05410 0.90 -0.3
Or see this region's nucleotide sequence