Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05370 and PGA1_c05380 are separated by 266 nucleotides PGA1_c05380 and PGA1_c05390 are separated by 161 nucleotides PGA1_c05390 and PGA1_c05400 are separated by 19 nucleotides
PGA1_c05370: PGA1_c05370 - polyketide synthase, at 538,451 to 544,939
_c05370
PGA1_c05380: PGA1_c05380 - hypothetical protein, at 545,206 to 546,669
_c05380
PGA1_c05390: PGA1_c05390 - glycosyl transferase, at 546,831 to 547,583
_c05390
PGA1_c05400: PGA1_c05400 - putative glycosyl transferase, family 2, at 547,603 to 548,586
_c05400
Position (kb) 545
546
547 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 544.275 kb on + strand, within PGA1_c05370 at 544.278 kb on - strand, within PGA1_c05370 at 544.285 kb on - strand, within PGA1_c05370 at 544.319 kb on - strand at 544.319 kb on - strand at 544.319 kb on - strand at 544.354 kb on + strand at 544.354 kb on + strand at 544.407 kb on + strand at 544.446 kb on + strand at 544.454 kb on - strand at 544.533 kb on - strand at 544.550 kb on - strand at 544.563 kb on - strand at 544.637 kb on - strand at 544.672 kb on - strand at 544.737 kb on + strand at 544.765 kb on + strand at 544.790 kb on - strand at 544.827 kb on + strand at 544.881 kb on + strand at 544.889 kb on - strand at 544.896 kb on + strand at 544.942 kb on + strand at 544.971 kb on + strand at 544.971 kb on + strand at 544.978 kb on + strand at 544.986 kb on - strand at 544.990 kb on + strand at 544.991 kb on - strand at 544.991 kb on - strand at 544.996 kb on - strand at 544.998 kb on - strand at 545.003 kb on - strand at 545.020 kb on - strand at 545.158 kb on + strand at 545.167 kb on - strand at 545.170 kb on + strand at 545.172 kb on - strand at 545.206 kb on + strand at 545.206 kb on + strand at 545.310 kb on + strand at 545.328 kb on - strand at 545.328 kb on - strand at 545.339 kb on - strand at 545.344 kb on - strand at 545.434 kb on + strand, within PGA1_c05380 at 545.458 kb on - strand, within PGA1_c05380 at 545.505 kb on - strand, within PGA1_c05380 at 545.528 kb on - strand, within PGA1_c05380 at 545.597 kb on + strand, within PGA1_c05380 at 545.705 kb on + strand, within PGA1_c05380 at 545.705 kb on - strand, within PGA1_c05380 at 545.719 kb on - strand, within PGA1_c05380 at 545.721 kb on + strand, within PGA1_c05380 at 545.734 kb on - strand, within PGA1_c05380 at 545.748 kb on + strand, within PGA1_c05380 at 545.748 kb on + strand, within PGA1_c05380 at 545.802 kb on - strand, within PGA1_c05380 at 545.876 kb on - strand, within PGA1_c05380 at 545.889 kb on + strand, within PGA1_c05380 at 545.896 kb on - strand, within PGA1_c05380 at 545.898 kb on - strand, within PGA1_c05380 at 545.931 kb on - strand, within PGA1_c05380 at 545.964 kb on + strand, within PGA1_c05380 at 545.998 kb on - strand, within PGA1_c05380 at 546.025 kb on + strand, within PGA1_c05380 at 546.025 kb on + strand, within PGA1_c05380 at 546.030 kb on + strand, within PGA1_c05380 at 546.033 kb on - strand, within PGA1_c05380 at 546.085 kb on + strand, within PGA1_c05380 at 546.085 kb on + strand, within PGA1_c05380 at 546.098 kb on + strand, within PGA1_c05380 at 546.106 kb on - strand, within PGA1_c05380 at 546.138 kb on + strand, within PGA1_c05380 at 546.148 kb on + strand, within PGA1_c05380 at 546.148 kb on + strand, within PGA1_c05380 at 546.148 kb on + strand, within PGA1_c05380 at 546.171 kb on - strand, within PGA1_c05380 at 546.205 kb on - strand, within PGA1_c05380 at 546.206 kb on + strand, within PGA1_c05380 at 546.235 kb on + strand, within PGA1_c05380 at 546.273 kb on - strand, within PGA1_c05380 at 546.348 kb on - strand, within PGA1_c05380 at 546.356 kb on + strand, within PGA1_c05380 at 546.364 kb on - strand, within PGA1_c05380 at 546.364 kb on - strand, within PGA1_c05380 at 546.364 kb on - strand, within PGA1_c05380 at 546.414 kb on + strand, within PGA1_c05380 at 546.464 kb on - strand, within PGA1_c05380 at 546.493 kb on - strand, within PGA1_c05380 at 546.534 kb on + strand at 546.534 kb on + strand at 546.534 kb on + strand at 546.542 kb on - strand at 546.542 kb on - strand at 546.542 kb on - strand at 546.542 kb on - strand at 546.555 kb on + strand at 546.574 kb on + strand at 546.582 kb on - strand at 546.612 kb on + strand at 546.612 kb on + strand at 546.612 kb on + strand at 546.612 kb on + strand at 546.620 kb on - strand at 546.620 kb on - strand at 546.642 kb on + strand at 546.642 kb on + strand at 546.649 kb on - strand at 546.650 kb on - strand at 546.652 kb on + strand at 546.686 kb on - strand at 546.795 kb on + strand at 546.838 kb on - strand at 546.861 kb on + strand at 546.861 kb on + strand at 546.957 kb on - strand, within PGA1_c05390 at 546.995 kb on + strand, within PGA1_c05390 at 546.995 kb on + strand, within PGA1_c05390 at 546.995 kb on + strand, within PGA1_c05390 at 547.003 kb on - strand, within PGA1_c05390 at 547.056 kb on - strand, within PGA1_c05390 at 547.136 kb on + strand, within PGA1_c05390 at 547.210 kb on + strand, within PGA1_c05390 at 547.219 kb on - strand, within PGA1_c05390 at 547.312 kb on + strand, within PGA1_c05390 at 547.407 kb on + strand, within PGA1_c05390 at 547.415 kb on - strand, within PGA1_c05390 at 547.415 kb on - strand, within PGA1_c05390 at 547.415 kb on - strand, within PGA1_c05390 at 547.512 kb on - strand at 547.520 kb on - strand at 547.536 kb on - strand at 547.569 kb on - strand at 547.580 kb on - strand at 547.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 544,275 + PGA1_c05370 0.90 +3.0 544,278 - PGA1_c05370 0.90 -2.1 544,285 - PGA1_c05370 0.90 +2.2 544,319 - -1.1 544,319 - -2.7 544,319 - +3.4 544,354 + +1.2 544,354 + -1.6 544,407 + +3.0 544,446 + -2.8 544,454 - -0.0 544,533 - -1.1 544,550 - +1.4 544,563 - +3.4 544,637 - +1.8 544,672 - +4.7 544,737 + +3.3 544,765 + +2.6 544,790 - +2.9 544,827 + +3.2 544,881 + -0.8 544,889 - +1.9 544,896 + +2.8 544,942 + -1.5 544,971 + -0.4 544,971 + -3.0 544,978 + -1.3 544,986 - +1.2 544,990 + +4.1 544,991 - -0.6 544,991 - -1.1 544,996 - -1.7 544,998 - -2.4 545,003 - -1.4 545,020 - +1.1 545,158 + -2.6 545,167 - +2.5 545,170 + +1.8 545,172 - -2.0 545,206 + -2.0 545,206 + +0.8 545,310 + -1.5 545,328 - -2.4 545,328 - +4.3 545,339 - +1.9 545,344 - -2.0 545,434 + PGA1_c05380 0.16 -3.1 545,458 - PGA1_c05380 0.17 -0.1 545,505 - PGA1_c05380 0.20 +2.0 545,528 - PGA1_c05380 0.22 -1.8 545,597 + PGA1_c05380 0.27 -1.0 545,705 + PGA1_c05380 0.34 -1.8 545,705 - PGA1_c05380 0.34 +3.4 545,719 - PGA1_c05380 0.35 +3.1 545,721 + PGA1_c05380 0.35 -0.8 545,734 - PGA1_c05380 0.36 -1.8 545,748 + PGA1_c05380 0.37 +1.7 545,748 + PGA1_c05380 0.37 -3.5 545,802 - PGA1_c05380 0.41 +2.1 545,876 - PGA1_c05380 0.46 -0.8 545,889 + PGA1_c05380 0.47 +4.0 545,896 - PGA1_c05380 0.47 -1.8 545,898 - PGA1_c05380 0.47 -1.0 545,931 - PGA1_c05380 0.50 +2.7 545,964 + PGA1_c05380 0.52 -1.1 545,998 - PGA1_c05380 0.54 -0.5 546,025 + PGA1_c05380 0.56 -2.5 546,025 + PGA1_c05380 0.56 -2.1 546,030 + PGA1_c05380 0.56 -1.8 546,033 - PGA1_c05380 0.56 -2.2 546,085 + PGA1_c05380 0.60 -2.3 546,085 + PGA1_c05380 0.60 -1.4 546,098 + PGA1_c05380 0.61 -1.4 546,106 - PGA1_c05380 0.61 -1.4 546,138 + PGA1_c05380 0.64 -0.6 546,148 + PGA1_c05380 0.64 -1.6 546,148 + PGA1_c05380 0.64 -2.5 546,148 + PGA1_c05380 0.64 -1.1 546,171 - PGA1_c05380 0.66 -2.9 546,205 - PGA1_c05380 0.68 +2.3 546,206 + PGA1_c05380 0.68 -0.9 546,235 + PGA1_c05380 0.70 +2.6 546,273 - PGA1_c05380 0.73 -0.5 546,348 - PGA1_c05380 0.78 -0.5 546,356 + PGA1_c05380 0.79 -1.6 546,364 - PGA1_c05380 0.79 -2.1 546,364 - PGA1_c05380 0.79 -0.3 546,364 - PGA1_c05380 0.79 -2.0 546,414 + PGA1_c05380 0.83 -1.7 546,464 - PGA1_c05380 0.86 -2.0 546,493 - PGA1_c05380 0.88 +1.7 546,534 + -2.2 546,534 + -0.8 546,534 + +2.2 546,542 - +3.4 546,542 - +0.4 546,542 - +1.9 546,542 - +3.7 546,555 + +1.0 546,574 + -1.2 546,582 - +2.3 546,612 + -1.8 546,612 + +1.1 546,612 + -2.0 546,612 + +1.4 546,620 - +0.8 546,620 - -2.3 546,642 + -1.0 546,642 + -1.4 546,649 - +3.7 546,650 - -1.5 546,652 + -0.9 546,686 - +1.4 546,795 + -0.8 546,838 - +1.8 546,861 + +2.0 546,861 + -2.9 546,957 - PGA1_c05390 0.17 +1.6 546,995 + PGA1_c05390 0.22 -2.2 546,995 + PGA1_c05390 0.22 -1.6 546,995 + PGA1_c05390 0.22 -1.2 547,003 - PGA1_c05390 0.23 +2.7 547,056 - PGA1_c05390 0.30 +2.2 547,136 + PGA1_c05390 0.41 +0.7 547,210 + PGA1_c05390 0.50 +0.9 547,219 - PGA1_c05390 0.52 -1.2 547,312 + PGA1_c05390 0.64 -2.0 547,407 + PGA1_c05390 0.76 -1.4 547,415 - PGA1_c05390 0.78 +2.6 547,415 - PGA1_c05390 0.78 -2.9 547,415 - PGA1_c05390 0.78 +2.0 547,512 - +4.2 547,520 - +3.6 547,536 - -1.4 547,569 - -0.7 547,580 - +0.9 547,585 - -1.3
Or see this region's nucleotide sequence