Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05330 and PGA1_c05340 overlap by 4 nucleotides PGA1_c05340 and PGA1_c05350 overlap by 4 nucleotides PGA1_c05350 and PGA1_c05360 overlap by 4 nucleotides
PGA1_c05330: PGA1_c05330 - hypothetical protein, at 531,144 to 532,046
_c05330
PGA1_c05340: PGA1_c05340 - 4'-phosphopantetheinyl transferase-like protein, at 532,043 to 532,756
_c05340
PGA1_c05350: PGA1_c05350 - dimodular nonribosomal peptide synthetase, at 532,753 to 537,387
_c05350
PGA1_c05360: PGA1_c05360 - putative glycosyl transferase, at 537,384 to 538,454
_c05360
Position (kb)
532
533
534
535
536
537
538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 531.801 kb on + strand, within PGA1_c05330 at 531.840 kb on - strand, within PGA1_c05330 at 531.902 kb on + strand, within PGA1_c05330 at 531.931 kb on - strand, within PGA1_c05330 at 531.960 kb on + strand at 531.982 kb on - strand at 532.017 kb on - strand at 532.782 kb on + strand at 532.865 kb on + strand at 532.905 kb on + strand at 532.995 kb on - strand at 533.035 kb on - strand at 533.046 kb on - strand at 533.249 kb on + strand, within PGA1_c05350 at 533.249 kb on + strand, within PGA1_c05350 at 533.249 kb on + strand, within PGA1_c05350 at 533.251 kb on + strand, within PGA1_c05350 at 533.257 kb on - strand, within PGA1_c05350 at 533.257 kb on - strand, within PGA1_c05350 at 533.259 kb on - strand, within PGA1_c05350 at 533.259 kb on - strand, within PGA1_c05350 at 533.396 kb on - strand, within PGA1_c05350 at 533.454 kb on + strand, within PGA1_c05350 at 533.454 kb on + strand, within PGA1_c05350 at 533.586 kb on + strand, within PGA1_c05350 at 533.636 kb on + strand, within PGA1_c05350 at 533.654 kb on + strand, within PGA1_c05350 at 533.719 kb on + strand, within PGA1_c05350 at 533.748 kb on + strand, within PGA1_c05350 at 533.781 kb on + strand, within PGA1_c05350 at 533.828 kb on + strand, within PGA1_c05350 at 533.893 kb on - strand, within PGA1_c05350 at 533.916 kb on + strand, within PGA1_c05350 at 534.018 kb on + strand, within PGA1_c05350 at 534.100 kb on - strand, within PGA1_c05350 at 534.139 kb on - strand, within PGA1_c05350 at 534.139 kb on - strand, within PGA1_c05350 at 534.148 kb on - strand, within PGA1_c05350 at 534.149 kb on - strand, within PGA1_c05350 at 534.175 kb on + strand, within PGA1_c05350 at 534.193 kb on - strand, within PGA1_c05350 at 534.225 kb on - strand, within PGA1_c05350 at 534.271 kb on - strand, within PGA1_c05350 at 534.386 kb on - strand, within PGA1_c05350 at 534.413 kb on - strand, within PGA1_c05350 at 534.469 kb on + strand, within PGA1_c05350 at 534.477 kb on - strand, within PGA1_c05350 at 534.589 kb on + strand, within PGA1_c05350 at 534.597 kb on - strand, within PGA1_c05350 at 534.633 kb on + strand, within PGA1_c05350 at 534.660 kb on + strand, within PGA1_c05350 at 534.674 kb on - strand, within PGA1_c05350 at 534.679 kb on - strand, within PGA1_c05350 at 534.679 kb on - strand, within PGA1_c05350 at 534.679 kb on - strand, within PGA1_c05350 at 534.748 kb on + strand, within PGA1_c05350 at 534.826 kb on - strand, within PGA1_c05350 at 534.831 kb on + strand, within PGA1_c05350 at 534.857 kb on - strand, within PGA1_c05350 at 534.947 kb on + strand, within PGA1_c05350 at 534.989 kb on + strand, within PGA1_c05350 at 534.989 kb on + strand, within PGA1_c05350 at 534.989 kb on + strand, within PGA1_c05350 at 535.002 kb on + strand, within PGA1_c05350 at 535.030 kb on - strand, within PGA1_c05350 at 535.042 kb on + strand, within PGA1_c05350 at 535.115 kb on + strand, within PGA1_c05350 at 535.118 kb on - strand, within PGA1_c05350 at 535.139 kb on - strand, within PGA1_c05350 at 535.151 kb on + strand, within PGA1_c05350 at 535.151 kb on + strand, within PGA1_c05350 at 535.151 kb on + strand, within PGA1_c05350 at 535.151 kb on + strand, within PGA1_c05350 at 535.151 kb on + strand, within PGA1_c05350 at 535.159 kb on - strand, within PGA1_c05350 at 535.159 kb on - strand, within PGA1_c05350 at 535.159 kb on - strand, within PGA1_c05350 at 535.159 kb on - strand, within PGA1_c05350 at 535.159 kb on - strand, within PGA1_c05350 at 535.294 kb on - strand, within PGA1_c05350 at 535.335 kb on + strand, within PGA1_c05350 at 535.335 kb on - strand, within PGA1_c05350 at 535.404 kb on + strand, within PGA1_c05350 at 535.426 kb on + strand, within PGA1_c05350 at 535.458 kb on + strand, within PGA1_c05350 at 535.491 kb on - strand, within PGA1_c05350 at 535.508 kb on + strand, within PGA1_c05350 at 535.511 kb on - strand, within PGA1_c05350 at 535.617 kb on + strand, within PGA1_c05350 at 535.687 kb on + strand, within PGA1_c05350 at 535.689 kb on - strand, within PGA1_c05350 at 535.689 kb on - strand, within PGA1_c05350 at 535.689 kb on - strand, within PGA1_c05350 at 535.724 kb on - strand, within PGA1_c05350 at 535.780 kb on - strand, within PGA1_c05350 at 535.800 kb on - strand, within PGA1_c05350 at 535.870 kb on + strand, within PGA1_c05350 at 535.900 kb on + strand, within PGA1_c05350 at 535.957 kb on - strand, within PGA1_c05350 at 536.127 kb on + strand, within PGA1_c05350 at 536.130 kb on - strand, within PGA1_c05350 at 536.163 kb on - strand, within PGA1_c05350 at 536.176 kb on + strand, within PGA1_c05350 at 536.248 kb on + strand, within PGA1_c05350 at 536.273 kb on - strand, within PGA1_c05350 at 536.273 kb on - strand, within PGA1_c05350 at 536.280 kb on + strand, within PGA1_c05350 at 536.280 kb on + strand, within PGA1_c05350 at 536.288 kb on - strand, within PGA1_c05350 at 536.328 kb on + strand, within PGA1_c05350 at 536.428 kb on - strand, within PGA1_c05350 at 536.557 kb on + strand, within PGA1_c05350 at 536.643 kb on - strand, within PGA1_c05350 at 536.669 kb on + strand, within PGA1_c05350 at 536.669 kb on + strand, within PGA1_c05350 at 536.731 kb on - strand, within PGA1_c05350 at 536.751 kb on + strand, within PGA1_c05350 at 536.825 kb on + strand, within PGA1_c05350 at 536.877 kb on + strand, within PGA1_c05350 at 536.923 kb on + strand, within PGA1_c05350 at 536.938 kb on + strand at 536.963 kb on + strand at 537.012 kb on + strand at 537.020 kb on - strand at 537.041 kb on + strand at 537.062 kb on + strand at 537.062 kb on + strand at 537.081 kb on - strand at 537.086 kb on - strand at 537.087 kb on - strand at 537.101 kb on - strand at 537.230 kb on + strand at 537.241 kb on + strand at 537.250 kb on - strand at 537.366 kb on + strand at 537.381 kb on - strand at 537.395 kb on + strand at 537.470 kb on - strand at 537.519 kb on - strand, within PGA1_c05360 at 537.524 kb on + strand, within PGA1_c05360 at 537.538 kb on + strand, within PGA1_c05360 at 537.538 kb on + strand, within PGA1_c05360 at 537.593 kb on + strand, within PGA1_c05360 at 537.636 kb on + strand, within PGA1_c05360 at 537.644 kb on - strand, within PGA1_c05360 at 537.687 kb on - strand, within PGA1_c05360 at 537.718 kb on - strand, within PGA1_c05360 at 537.720 kb on + strand, within PGA1_c05360 at 537.742 kb on + strand, within PGA1_c05360 at 537.742 kb on + strand, within PGA1_c05360 at 537.750 kb on - strand, within PGA1_c05360 at 537.785 kb on + strand, within PGA1_c05360 at 537.793 kb on - strand, within PGA1_c05360 at 537.844 kb on + strand, within PGA1_c05360 at 537.857 kb on + strand, within PGA1_c05360 at 537.868 kb on + strand, within PGA1_c05360 at 537.919 kb on - strand, within PGA1_c05360 at 538.004 kb on + strand, within PGA1_c05360 at 538.012 kb on - strand, within PGA1_c05360 at 538.012 kb on - strand, within PGA1_c05360 at 538.012 kb on - strand, within PGA1_c05360 at 538.027 kb on - strand, within PGA1_c05360 at 538.027 kb on - strand, within PGA1_c05360 at 538.074 kb on - strand, within PGA1_c05360 at 538.093 kb on - strand, within PGA1_c05360 at 538.102 kb on + strand, within PGA1_c05360 at 538.141 kb on - strand, within PGA1_c05360 at 538.209 kb on + strand, within PGA1_c05360 at 538.237 kb on + strand, within PGA1_c05360 at 538.297 kb on - strand, within PGA1_c05360 at 538.315 kb on - strand, within PGA1_c05360 at 538.333 kb on + strand, within PGA1_c05360 at 538.338 kb on + strand, within PGA1_c05360 at 538.341 kb on - strand, within PGA1_c05360 at 538.347 kb on + strand, within PGA1_c05360 at 538.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 531,801 + PGA1_c05330 0.73 -0.3 531,840 - PGA1_c05330 0.77 -0.3 531,902 + PGA1_c05330 0.84 +2.7 531,931 - PGA1_c05330 0.87 +0.4 531,960 + -2.5 531,982 - -1.5 532,017 - -2.3 532,782 + +2.4 532,865 + -0.9 532,905 + +1.5 532,995 - +2.9 533,035 - +3.2 533,046 - +1.4 533,249 + PGA1_c05350 0.11 -0.6 533,249 + PGA1_c05350 0.11 +2.9 533,249 + PGA1_c05350 0.11 +2.1 533,251 + PGA1_c05350 0.11 +1.9 533,257 - PGA1_c05350 0.11 +3.0 533,257 - PGA1_c05350 0.11 -1.8 533,259 - PGA1_c05350 0.11 -1.8 533,259 - PGA1_c05350 0.11 -0.3 533,396 - PGA1_c05350 0.14 +0.7 533,454 + PGA1_c05350 0.15 +1.5 533,454 + PGA1_c05350 0.15 +2.6 533,586 + PGA1_c05350 0.18 +1.3 533,636 + PGA1_c05350 0.19 +1.7 533,654 + PGA1_c05350 0.19 -2.0 533,719 + PGA1_c05350 0.21 +2.7 533,748 + PGA1_c05350 0.21 +1.1 533,781 + PGA1_c05350 0.22 +1.3 533,828 + PGA1_c05350 0.23 +2.1 533,893 - PGA1_c05350 0.25 +1.9 533,916 + PGA1_c05350 0.25 +3.0 534,018 + PGA1_c05350 0.27 -0.6 534,100 - PGA1_c05350 0.29 -1.2 534,139 - PGA1_c05350 0.30 +2.3 534,139 - PGA1_c05350 0.30 +1.9 534,148 - PGA1_c05350 0.30 +0.5 534,149 - PGA1_c05350 0.30 -2.0 534,175 + PGA1_c05350 0.31 -1.8 534,193 - PGA1_c05350 0.31 +1.8 534,225 - PGA1_c05350 0.32 -0.8 534,271 - PGA1_c05350 0.33 +3.8 534,386 - PGA1_c05350 0.35 +2.6 534,413 - PGA1_c05350 0.36 +0.3 534,469 + PGA1_c05350 0.37 -1.6 534,477 - PGA1_c05350 0.37 +2.0 534,589 + PGA1_c05350 0.40 +0.8 534,597 - PGA1_c05350 0.40 -2.4 534,633 + PGA1_c05350 0.41 -1.9 534,660 + PGA1_c05350 0.41 +1.2 534,674 - PGA1_c05350 0.41 +2.9 534,679 - PGA1_c05350 0.42 +2.8 534,679 - PGA1_c05350 0.42 -0.6 534,679 - PGA1_c05350 0.42 -1.6 534,748 + PGA1_c05350 0.43 -1.6 534,826 - PGA1_c05350 0.45 +3.2 534,831 + PGA1_c05350 0.45 -1.6 534,857 - PGA1_c05350 0.45 -2.0 534,947 + PGA1_c05350 0.47 +0.3 534,989 + PGA1_c05350 0.48 +1.5 534,989 + PGA1_c05350 0.48 +0.3 534,989 + PGA1_c05350 0.48 +3.7 535,002 + PGA1_c05350 0.49 +1.3 535,030 - PGA1_c05350 0.49 +1.9 535,042 + PGA1_c05350 0.49 +1.5 535,115 + PGA1_c05350 0.51 -1.4 535,118 - PGA1_c05350 0.51 -1.1 535,139 - PGA1_c05350 0.51 +2.1 535,151 + PGA1_c05350 0.52 +2.7 535,151 + PGA1_c05350 0.52 -2.0 535,151 + PGA1_c05350 0.52 +2.4 535,151 + PGA1_c05350 0.52 +3.7 535,151 + PGA1_c05350 0.52 -0.6 535,159 - PGA1_c05350 0.52 +3.4 535,159 - PGA1_c05350 0.52 -0.8 535,159 - PGA1_c05350 0.52 +0.7 535,159 - PGA1_c05350 0.52 -1.7 535,159 - PGA1_c05350 0.52 +2.1 535,294 - PGA1_c05350 0.55 +4.5 535,335 + PGA1_c05350 0.56 +1.0 535,335 - PGA1_c05350 0.56 -0.8 535,404 + PGA1_c05350 0.57 +0.9 535,426 + PGA1_c05350 0.58 +1.6 535,458 + PGA1_c05350 0.58 -2.3 535,491 - PGA1_c05350 0.59 +2.5 535,508 + PGA1_c05350 0.59 +1.5 535,511 - PGA1_c05350 0.60 -0.1 535,617 + PGA1_c05350 0.62 +2.6 535,687 + PGA1_c05350 0.63 +0.4 535,689 - PGA1_c05350 0.63 +3.3 535,689 - PGA1_c05350 0.63 +0.5 535,689 - PGA1_c05350 0.63 +3.0 535,724 - PGA1_c05350 0.64 +1.9 535,780 - PGA1_c05350 0.65 -1.7 535,800 - PGA1_c05350 0.66 +2.9 535,870 + PGA1_c05350 0.67 -2.0 535,900 + PGA1_c05350 0.68 -2.4 535,957 - PGA1_c05350 0.69 +2.5 536,127 + PGA1_c05350 0.73 +2.4 536,130 - PGA1_c05350 0.73 +1.8 536,163 - PGA1_c05350 0.74 -1.1 536,176 + PGA1_c05350 0.74 +1.5 536,248 + PGA1_c05350 0.75 -1.9 536,273 - PGA1_c05350 0.76 +0.5 536,273 - PGA1_c05350 0.76 +1.8 536,280 + PGA1_c05350 0.76 -1.2 536,280 + PGA1_c05350 0.76 -1.6 536,288 - PGA1_c05350 0.76 +1.0 536,328 + PGA1_c05350 0.77 +1.4 536,428 - PGA1_c05350 0.79 -1.9 536,557 + PGA1_c05350 0.82 -2.2 536,643 - PGA1_c05350 0.84 +2.9 536,669 + PGA1_c05350 0.84 +1.3 536,669 + PGA1_c05350 0.84 -1.0 536,731 - PGA1_c05350 0.86 -2.2 536,751 + PGA1_c05350 0.86 -1.2 536,825 + PGA1_c05350 0.88 +0.9 536,877 + PGA1_c05350 0.89 +1.6 536,923 + PGA1_c05350 0.90 +1.8 536,938 + +2.3 536,963 + +2.3 537,012 + +3.2 537,020 - +1.2 537,041 + +0.6 537,062 + +2.8 537,062 + +0.9 537,081 - +1.2 537,086 - +2.9 537,087 - +3.9 537,101 - -2.6 537,230 + +3.2 537,241 + -2.4 537,250 - +1.8 537,366 + -1.3 537,381 - +1.6 537,395 + +0.7 537,470 - +1.7 537,519 - PGA1_c05360 0.13 +2.5 537,524 + PGA1_c05360 0.13 +0.8 537,538 + PGA1_c05360 0.14 +4.3 537,538 + PGA1_c05360 0.14 -1.5 537,593 + PGA1_c05360 0.20 +3.4 537,636 + PGA1_c05360 0.24 -1.4 537,644 - PGA1_c05360 0.24 -1.3 537,687 - PGA1_c05360 0.28 -2.7 537,718 - PGA1_c05360 0.31 -1.1 537,720 + PGA1_c05360 0.31 +1.5 537,742 + PGA1_c05360 0.33 +2.1 537,742 + PGA1_c05360 0.33 +3.0 537,750 - PGA1_c05360 0.34 -1.9 537,785 + PGA1_c05360 0.37 +3.7 537,793 - PGA1_c05360 0.38 +0.2 537,844 + PGA1_c05360 0.43 +2.3 537,857 + PGA1_c05360 0.44 +3.5 537,868 + PGA1_c05360 0.45 +3.7 537,919 - PGA1_c05360 0.50 +1.2 538,004 + PGA1_c05360 0.58 -1.0 538,012 - PGA1_c05360 0.59 -1.9 538,012 - PGA1_c05360 0.59 +1.6 538,012 - PGA1_c05360 0.59 +2.6 538,027 - PGA1_c05360 0.60 +1.5 538,027 - PGA1_c05360 0.60 +1.3 538,074 - PGA1_c05360 0.64 -1.8 538,093 - PGA1_c05360 0.66 +3.0 538,102 + PGA1_c05360 0.67 -0.4 538,141 - PGA1_c05360 0.71 +1.5 538,209 + PGA1_c05360 0.77 -2.8 538,237 + PGA1_c05360 0.80 -1.7 538,297 - PGA1_c05360 0.85 -2.0 538,315 - PGA1_c05360 0.87 +0.6 538,333 + PGA1_c05360 0.89 -2.2 538,338 + PGA1_c05360 0.89 -1.9 538,341 - PGA1_c05360 0.89 -1.4 538,347 + PGA1_c05360 0.90 +2.7 538,355 + +1.4
Or see this region's nucleotide sequence