Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05230 and PGA1_c05240 are separated by 327 nucleotides PGA1_c05240 and PGA1_c05250 are separated by 31 nucleotides
PGA1_c05230: PGA1_c05230 - ABC transporter, extracellular solute-binding protein, at 516,786 to 518,519
_c05230
PGA1_c05240: PGA1_c05240 - Predicted N6-adenine-specific DNA methylase, at 518,847 to 519,977
_c05240
PGA1_c05250: PGA1_c05250 - 2-keto-3-deoxy-galactonokinase, at 520,009 to 520,998
_c05250
Position (kb) 518
519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 517.857 kb on + strand, within PGA1_c05230 at 517.878 kb on + strand, within PGA1_c05230 at 517.878 kb on - strand, within PGA1_c05230 at 517.886 kb on + strand, within PGA1_c05230 at 517.900 kb on - strand, within PGA1_c05230 at 517.931 kb on - strand, within PGA1_c05230 at 517.931 kb on - strand, within PGA1_c05230 at 517.960 kb on + strand, within PGA1_c05230 at 517.960 kb on + strand, within PGA1_c05230 at 517.968 kb on - strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.032 kb on + strand, within PGA1_c05230 at 518.040 kb on - strand, within PGA1_c05230 at 518.072 kb on - strand, within PGA1_c05230 at 518.097 kb on + strand, within PGA1_c05230 at 518.131 kb on + strand, within PGA1_c05230 at 518.139 kb on - strand, within PGA1_c05230 at 518.149 kb on - strand, within PGA1_c05230 at 518.149 kb on - strand, within PGA1_c05230 at 518.186 kb on + strand, within PGA1_c05230 at 518.193 kb on + strand, within PGA1_c05230 at 518.193 kb on + strand, within PGA1_c05230 at 518.193 kb on + strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.211 kb on - strand, within PGA1_c05230 at 518.211 kb on - strand, within PGA1_c05230 at 518.211 kb on - strand, within PGA1_c05230 at 518.229 kb on + strand, within PGA1_c05230 at 518.243 kb on + strand, within PGA1_c05230 at 518.274 kb on + strand, within PGA1_c05230 at 518.274 kb on + strand, within PGA1_c05230 at 518.274 kb on + strand, within PGA1_c05230 at 518.311 kb on + strand, within PGA1_c05230 at 518.311 kb on + strand, within PGA1_c05230 at 518.311 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.348 kb on + strand at 518.355 kb on - strand at 518.356 kb on - strand at 518.356 kb on - strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.400 kb on - strand at 518.454 kb on + strand at 518.458 kb on - strand at 518.462 kb on - strand at 518.512 kb on - strand at 518.536 kb on + strand at 518.536 kb on + strand at 518.544 kb on - strand at 518.583 kb on + strand at 518.591 kb on - strand at 518.680 kb on - strand at 518.693 kb on + strand at 518.694 kb on - strand at 518.701 kb on - strand at 518.731 kb on + strand at 518.803 kb on + strand at 518.838 kb on + strand at 518.898 kb on + strand at 518.967 kb on + strand, within PGA1_c05240 at 518.976 kb on - strand, within PGA1_c05240 at 519.024 kb on + strand, within PGA1_c05240 at 519.041 kb on + strand, within PGA1_c05240 at 519.058 kb on + strand, within PGA1_c05240 at 519.058 kb on + strand, within PGA1_c05240 at 519.066 kb on - strand, within PGA1_c05240 at 519.113 kb on + strand, within PGA1_c05240 at 519.113 kb on + strand, within PGA1_c05240 at 519.118 kb on + strand, within PGA1_c05240 at 519.134 kb on - strand, within PGA1_c05240 at 519.206 kb on + strand, within PGA1_c05240 at 519.206 kb on + strand, within PGA1_c05240 at 519.214 kb on - strand, within PGA1_c05240 at 519.214 kb on - strand, within PGA1_c05240 at 519.233 kb on + strand, within PGA1_c05240 at 519.256 kb on + strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.273 kb on + strand, within PGA1_c05240 at 519.306 kb on - strand, within PGA1_c05240 at 519.328 kb on - strand, within PGA1_c05240 at 519.342 kb on + strand, within PGA1_c05240 at 519.347 kb on + strand, within PGA1_c05240 at 519.434 kb on - strand, within PGA1_c05240 at 519.455 kb on + strand, within PGA1_c05240 at 519.545 kb on - strand, within PGA1_c05240 at 519.545 kb on - strand, within PGA1_c05240 at 519.632 kb on - strand, within PGA1_c05240 at 519.651 kb on - strand, within PGA1_c05240 at 519.665 kb on - strand, within PGA1_c05240 at 519.717 kb on - strand, within PGA1_c05240 at 519.791 kb on + strand, within PGA1_c05240 at 519.799 kb on - strand, within PGA1_c05240 at 519.856 kb on + strand, within PGA1_c05240 at 519.876 kb on - strand at 519.881 kb on + strand at 519.881 kb on + strand at 519.881 kb on + strand at 519.894 kb on + strand at 519.894 kb on + strand at 519.909 kb on + strand at 519.909 kb on + strand at 519.973 kb on + strand at 519.988 kb on - strand at 520.053 kb on - strand at 520.104 kb on - strand at 520.118 kb on + strand, within PGA1_c05250 at 520.133 kb on + strand, within PGA1_c05250 at 520.151 kb on - strand, within PGA1_c05250 at 520.158 kb on + strand, within PGA1_c05250 at 520.213 kb on - strand, within PGA1_c05250 at 520.220 kb on + strand, within PGA1_c05250 at 520.220 kb on + strand, within PGA1_c05250 at 520.314 kb on - strand, within PGA1_c05250 at 520.321 kb on - strand, within PGA1_c05250 at 520.374 kb on + strand, within PGA1_c05250 at 520.374 kb on + strand, within PGA1_c05250 at 520.374 kb on + strand, within PGA1_c05250 at 520.374 kb on + strand, within PGA1_c05250 at 520.374 kb on + strand, within PGA1_c05250 at 520.374 kb on + strand, within PGA1_c05250 at 520.382 kb on - strand, within PGA1_c05250 at 520.418 kb on + strand, within PGA1_c05250 at 520.446 kb on - strand, within PGA1_c05250 at 520.446 kb on - strand, within PGA1_c05250 at 520.452 kb on - strand, within PGA1_c05250 at 520.544 kb on - strand, within PGA1_c05250 at 520.604 kb on + strand, within PGA1_c05250 at 520.618 kb on - strand, within PGA1_c05250 at 520.645 kb on - strand, within PGA1_c05250 at 520.648 kb on - strand, within PGA1_c05250 at 520.704 kb on - strand, within PGA1_c05250 at 520.726 kb on + strand, within PGA1_c05250 at 520.788 kb on + strand, within PGA1_c05250 at 520.792 kb on + strand, within PGA1_c05250 at 520.792 kb on + strand, within PGA1_c05250 at 520.794 kb on - strand, within PGA1_c05250 at 520.796 kb on - strand, within PGA1_c05250 at 520.811 kb on + strand, within PGA1_c05250 at 520.869 kb on + strand, within PGA1_c05250 at 520.935 kb on - strand at 520.944 kb on + strand at 520.945 kb on + strand at 520.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 517,857 + PGA1_c05230 0.62 +4.2 517,878 + PGA1_c05230 0.63 +1.3 517,878 - PGA1_c05230 0.63 +0.7 517,886 + PGA1_c05230 0.63 -1.9 517,900 - PGA1_c05230 0.64 -0.2 517,931 - PGA1_c05230 0.66 +1.5 517,931 - PGA1_c05230 0.66 -1.5 517,960 + PGA1_c05230 0.68 +3.2 517,960 + PGA1_c05230 0.68 +0.3 517,968 - PGA1_c05230 0.68 -0.5 518,017 + PGA1_c05230 0.71 -2.3 518,017 + PGA1_c05230 0.71 +3.4 518,017 + PGA1_c05230 0.71 -2.1 518,017 + PGA1_c05230 0.71 +0.3 518,017 + PGA1_c05230 0.71 -1.9 518,025 - PGA1_c05230 0.71 +1.1 518,025 - PGA1_c05230 0.71 -1.2 518,025 - PGA1_c05230 0.71 +1.4 518,025 - PGA1_c05230 0.71 +2.2 518,025 - PGA1_c05230 0.71 -1.6 518,025 - PGA1_c05230 0.71 +1.2 518,025 - PGA1_c05230 0.71 +2.0 518,032 + PGA1_c05230 0.72 -1.9 518,040 - PGA1_c05230 0.72 +2.7 518,072 - PGA1_c05230 0.74 -0.1 518,097 + PGA1_c05230 0.76 +3.2 518,131 + PGA1_c05230 0.78 +1.8 518,139 - PGA1_c05230 0.78 -2.5 518,149 - PGA1_c05230 0.79 -1.1 518,149 - PGA1_c05230 0.79 +2.4 518,186 + PGA1_c05230 0.81 -1.0 518,193 + PGA1_c05230 0.81 +1.7 518,193 + PGA1_c05230 0.81 +1.4 518,193 + PGA1_c05230 0.81 +0.8 518,201 - PGA1_c05230 0.82 -0.6 518,201 - PGA1_c05230 0.82 +0.3 518,201 - PGA1_c05230 0.82 +2.4 518,201 - PGA1_c05230 0.82 +2.5 518,201 - PGA1_c05230 0.82 +0.9 518,211 - PGA1_c05230 0.82 -0.9 518,211 - PGA1_c05230 0.82 +1.7 518,211 - PGA1_c05230 0.82 +1.8 518,229 + PGA1_c05230 0.83 +2.6 518,243 + PGA1_c05230 0.84 -1.0 518,274 + PGA1_c05230 0.86 -1.5 518,274 + PGA1_c05230 0.86 -0.5 518,274 + PGA1_c05230 0.86 -0.1 518,311 + PGA1_c05230 0.88 -2.6 518,311 + PGA1_c05230 0.88 -2.2 518,311 + PGA1_c05230 0.88 +2.1 518,338 + PGA1_c05230 0.90 +0.9 518,338 + PGA1_c05230 0.90 -0.1 518,338 + PGA1_c05230 0.90 -0.8 518,338 + PGA1_c05230 0.90 -2.3 518,338 + PGA1_c05230 0.90 +0.8 518,346 - -0.0 518,346 - +2.4 518,346 - +1.9 518,346 - -1.8 518,346 - +3.8 518,346 - +0.4 518,348 + +2.7 518,355 - +4.1 518,356 - -0.0 518,356 - +1.3 518,397 + +3.4 518,397 + +2.9 518,397 + +1.8 518,400 - -1.2 518,454 + -1.7 518,458 - +2.8 518,462 - +1.9 518,512 - +1.8 518,536 + -2.0 518,536 + -1.1 518,544 - -0.7 518,583 + +0.4 518,591 - -1.3 518,680 - -2.2 518,693 + +0.6 518,694 - +3.8 518,701 - +0.2 518,731 + -1.9 518,803 + -1.6 518,838 + +2.8 518,898 + -1.7 518,967 + PGA1_c05240 0.11 -1.3 518,976 - PGA1_c05240 0.11 +2.2 519,024 + PGA1_c05240 0.16 +1.0 519,041 + PGA1_c05240 0.17 +2.4 519,058 + PGA1_c05240 0.19 -1.6 519,058 + PGA1_c05240 0.19 -0.5 519,066 - PGA1_c05240 0.19 +0.2 519,113 + PGA1_c05240 0.24 +4.8 519,113 + PGA1_c05240 0.24 +2.1 519,118 + PGA1_c05240 0.24 -0.4 519,134 - PGA1_c05240 0.25 -2.0 519,206 + PGA1_c05240 0.32 +2.0 519,206 + PGA1_c05240 0.32 -2.3 519,214 - PGA1_c05240 0.32 -2.7 519,214 - PGA1_c05240 0.32 -2.0 519,233 + PGA1_c05240 0.34 +4.2 519,256 + PGA1_c05240 0.36 +1.1 519,264 - PGA1_c05240 0.37 +2.8 519,264 - PGA1_c05240 0.37 +1.5 519,264 - PGA1_c05240 0.37 +0.8 519,264 - PGA1_c05240 0.37 -2.2 519,273 + PGA1_c05240 0.38 +2.4 519,306 - PGA1_c05240 0.41 -2.7 519,328 - PGA1_c05240 0.43 +4.3 519,342 + PGA1_c05240 0.44 -1.8 519,347 + PGA1_c05240 0.44 +2.5 519,434 - PGA1_c05240 0.52 +1.9 519,455 + PGA1_c05240 0.54 +1.4 519,545 - PGA1_c05240 0.62 -1.1 519,545 - PGA1_c05240 0.62 +2.2 519,632 - PGA1_c05240 0.69 -1.8 519,651 - PGA1_c05240 0.71 -1.1 519,665 - PGA1_c05240 0.72 -0.8 519,717 - PGA1_c05240 0.77 -1.9 519,791 + PGA1_c05240 0.83 -2.1 519,799 - PGA1_c05240 0.84 +2.9 519,856 + PGA1_c05240 0.89 +0.5 519,876 - -1.9 519,881 + -1.9 519,881 + +0.7 519,881 + -1.4 519,894 + -2.3 519,894 + +2.0 519,909 + +2.4 519,909 + -0.0 519,973 + +0.5 519,988 - +0.6 520,053 - -0.7 520,104 - -1.8 520,118 + PGA1_c05250 0.11 +1.8 520,133 + PGA1_c05250 0.13 -1.9 520,151 - PGA1_c05250 0.14 -2.1 520,158 + PGA1_c05250 0.15 -0.1 520,213 - PGA1_c05250 0.21 -2.0 520,220 + PGA1_c05250 0.21 +1.0 520,220 + PGA1_c05250 0.21 -1.0 520,314 - PGA1_c05250 0.31 -3.2 520,321 - PGA1_c05250 0.32 +1.8 520,374 + PGA1_c05250 0.37 +2.6 520,374 + PGA1_c05250 0.37 +3.1 520,374 + PGA1_c05250 0.37 -2.4 520,374 + PGA1_c05250 0.37 +1.2 520,374 + PGA1_c05250 0.37 +2.3 520,374 + PGA1_c05250 0.37 -1.0 520,382 - PGA1_c05250 0.38 +2.1 520,418 + PGA1_c05250 0.41 -2.0 520,446 - PGA1_c05250 0.44 +3.3 520,446 - PGA1_c05250 0.44 +0.5 520,452 - PGA1_c05250 0.45 +1.9 520,544 - PGA1_c05250 0.54 +1.6 520,604 + PGA1_c05250 0.60 +2.3 520,618 - PGA1_c05250 0.62 -1.0 520,645 - PGA1_c05250 0.64 +1.5 520,648 - PGA1_c05250 0.65 +1.4 520,704 - PGA1_c05250 0.70 +2.6 520,726 + PGA1_c05250 0.72 +1.4 520,788 + PGA1_c05250 0.79 +1.9 520,792 + PGA1_c05250 0.79 -0.6 520,792 + PGA1_c05250 0.79 +1.7 520,794 - PGA1_c05250 0.79 -2.2 520,796 - PGA1_c05250 0.79 +2.1 520,811 + PGA1_c05250 0.81 -1.5 520,869 + PGA1_c05250 0.87 +0.3 520,935 - +2.0 520,944 + -1.1 520,945 + +1.0 520,947 - -0.4
Or see this region's nucleotide sequence