Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05220 and PGA1_c05230 are separated by 111 nucleotides PGA1_c05230 and PGA1_c05240 are separated by 327 nucleotides
PGA1_c05220: PGA1_c05220 - putative sn-glycerol-3-phosphate import ATP-binding protein UgpC, at 515,556 to 516,674
_c05220
PGA1_c05230: PGA1_c05230 - ABC transporter, extracellular solute-binding protein, at 516,786 to 518,519
_c05230
PGA1_c05240: PGA1_c05240 - Predicted N6-adenine-specific DNA methylase, at 518,847 to 519,977
_c05240
Position (kb) 516
517
518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 515.800 kb on + strand, within PGA1_c05220 at 515.862 kb on + strand, within PGA1_c05220 at 515.870 kb on - strand, within PGA1_c05220 at 515.882 kb on + strand, within PGA1_c05220 at 515.887 kb on + strand, within PGA1_c05220 at 515.887 kb on + strand, within PGA1_c05220 at 515.887 kb on + strand, within PGA1_c05220 at 515.887 kb on + strand, within PGA1_c05220 at 515.887 kb on + strand, within PGA1_c05220 at 515.887 kb on + strand, within PGA1_c05220 at 515.895 kb on - strand, within PGA1_c05220 at 515.895 kb on - strand, within PGA1_c05220 at 515.948 kb on + strand, within PGA1_c05220 at 515.953 kb on + strand, within PGA1_c05220 at 515.956 kb on - strand, within PGA1_c05220 at 516.004 kb on + strand, within PGA1_c05220 at 516.010 kb on + strand, within PGA1_c05220 at 516.018 kb on - strand, within PGA1_c05220 at 516.039 kb on - strand, within PGA1_c05220 at 516.077 kb on + strand, within PGA1_c05220 at 516.204 kb on - strand, within PGA1_c05220 at 516.209 kb on - strand, within PGA1_c05220 at 516.209 kb on - strand, within PGA1_c05220 at 516.219 kb on + strand, within PGA1_c05220 at 516.219 kb on + strand, within PGA1_c05220 at 516.257 kb on + strand, within PGA1_c05220 at 516.372 kb on - strand, within PGA1_c05220 at 516.431 kb on + strand, within PGA1_c05220 at 516.441 kb on - strand, within PGA1_c05220 at 516.587 kb on + strand at 516.592 kb on + strand at 516.711 kb on - strand at 516.751 kb on + strand at 516.768 kb on + strand at 516.783 kb on + strand at 516.783 kb on - strand at 516.791 kb on - strand at 516.815 kb on + strand at 516.829 kb on + strand at 516.837 kb on - strand at 516.945 kb on - strand at 516.958 kb on - strand at 516.962 kb on + strand, within PGA1_c05230 at 516.969 kb on + strand, within PGA1_c05230 at 516.970 kb on - strand, within PGA1_c05230 at 517.002 kb on + strand, within PGA1_c05230 at 517.002 kb on + strand, within PGA1_c05230 at 517.033 kb on + strand, within PGA1_c05230 at 517.041 kb on - strand, within PGA1_c05230 at 517.060 kb on - strand, within PGA1_c05230 at 517.070 kb on - strand, within PGA1_c05230 at 517.073 kb on + strand, within PGA1_c05230 at 517.073 kb on + strand, within PGA1_c05230 at 517.073 kb on + strand, within PGA1_c05230 at 517.091 kb on + strand, within PGA1_c05230 at 517.110 kb on - strand, within PGA1_c05230 at 517.173 kb on + strand, within PGA1_c05230 at 517.180 kb on - strand, within PGA1_c05230 at 517.198 kb on - strand, within PGA1_c05230 at 517.212 kb on + strand, within PGA1_c05230 at 517.212 kb on + strand, within PGA1_c05230 at 517.220 kb on - strand, within PGA1_c05230 at 517.246 kb on + strand, within PGA1_c05230 at 517.246 kb on + strand, within PGA1_c05230 at 517.246 kb on + strand, within PGA1_c05230 at 517.246 kb on + strand, within PGA1_c05230 at 517.254 kb on - strand, within PGA1_c05230 at 517.254 kb on - strand, within PGA1_c05230 at 517.254 kb on - strand, within PGA1_c05230 at 517.254 kb on - strand, within PGA1_c05230 at 517.254 kb on - strand, within PGA1_c05230 at 517.256 kb on - strand, within PGA1_c05230 at 517.318 kb on + strand, within PGA1_c05230 at 517.365 kb on + strand, within PGA1_c05230 at 517.378 kb on - strand, within PGA1_c05230 at 517.503 kb on - strand, within PGA1_c05230 at 517.505 kb on + strand, within PGA1_c05230 at 517.505 kb on + strand, within PGA1_c05230 at 517.513 kb on - strand, within PGA1_c05230 at 517.590 kb on - strand, within PGA1_c05230 at 517.761 kb on - strand, within PGA1_c05230 at 517.769 kb on + strand, within PGA1_c05230 at 517.826 kb on + strand, within PGA1_c05230 at 517.857 kb on + strand, within PGA1_c05230 at 517.878 kb on + strand, within PGA1_c05230 at 517.878 kb on - strand, within PGA1_c05230 at 517.886 kb on + strand, within PGA1_c05230 at 517.900 kb on - strand, within PGA1_c05230 at 517.931 kb on - strand, within PGA1_c05230 at 517.931 kb on - strand, within PGA1_c05230 at 517.960 kb on + strand, within PGA1_c05230 at 517.960 kb on + strand, within PGA1_c05230 at 517.968 kb on - strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.017 kb on + strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.025 kb on - strand, within PGA1_c05230 at 518.032 kb on + strand, within PGA1_c05230 at 518.040 kb on - strand, within PGA1_c05230 at 518.072 kb on - strand, within PGA1_c05230 at 518.097 kb on + strand, within PGA1_c05230 at 518.131 kb on + strand, within PGA1_c05230 at 518.139 kb on - strand, within PGA1_c05230 at 518.149 kb on - strand, within PGA1_c05230 at 518.149 kb on - strand, within PGA1_c05230 at 518.186 kb on + strand, within PGA1_c05230 at 518.193 kb on + strand, within PGA1_c05230 at 518.193 kb on + strand, within PGA1_c05230 at 518.193 kb on + strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.201 kb on - strand, within PGA1_c05230 at 518.211 kb on - strand, within PGA1_c05230 at 518.211 kb on - strand, within PGA1_c05230 at 518.211 kb on - strand, within PGA1_c05230 at 518.229 kb on + strand, within PGA1_c05230 at 518.243 kb on + strand, within PGA1_c05230 at 518.274 kb on + strand, within PGA1_c05230 at 518.274 kb on + strand, within PGA1_c05230 at 518.274 kb on + strand, within PGA1_c05230 at 518.311 kb on + strand, within PGA1_c05230 at 518.311 kb on + strand, within PGA1_c05230 at 518.311 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.338 kb on + strand, within PGA1_c05230 at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.346 kb on - strand at 518.348 kb on + strand at 518.355 kb on - strand at 518.356 kb on - strand at 518.356 kb on - strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.400 kb on - strand at 518.454 kb on + strand at 518.458 kb on - strand at 518.462 kb on - strand at 518.512 kb on - strand at 518.536 kb on + strand at 518.536 kb on + strand at 518.544 kb on - strand at 518.583 kb on + strand at 518.591 kb on - strand at 518.680 kb on - strand at 518.693 kb on + strand at 518.694 kb on - strand at 518.701 kb on - strand at 518.731 kb on + strand at 518.803 kb on + strand at 518.838 kb on + strand at 518.898 kb on + strand at 518.967 kb on + strand, within PGA1_c05240 at 518.976 kb on - strand, within PGA1_c05240 at 519.024 kb on + strand, within PGA1_c05240 at 519.041 kb on + strand, within PGA1_c05240 at 519.058 kb on + strand, within PGA1_c05240 at 519.058 kb on + strand, within PGA1_c05240 at 519.066 kb on - strand, within PGA1_c05240 at 519.113 kb on + strand, within PGA1_c05240 at 519.113 kb on + strand, within PGA1_c05240 at 519.118 kb on + strand, within PGA1_c05240 at 519.134 kb on - strand, within PGA1_c05240 at 519.206 kb on + strand, within PGA1_c05240 at 519.206 kb on + strand, within PGA1_c05240 at 519.214 kb on - strand, within PGA1_c05240 at 519.214 kb on - strand, within PGA1_c05240 at 519.233 kb on + strand, within PGA1_c05240 at 519.256 kb on + strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.264 kb on - strand, within PGA1_c05240 at 519.273 kb on + strand, within PGA1_c05240 at 519.306 kb on - strand, within PGA1_c05240 at 519.328 kb on - strand, within PGA1_c05240 at 519.342 kb on + strand, within PGA1_c05240 at 519.347 kb on + strand, within PGA1_c05240 at 519.434 kb on - strand, within PGA1_c05240 at 519.455 kb on + strand, within PGA1_c05240
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 515,800 + PGA1_c05220 0.22 -0.9 515,862 + PGA1_c05220 0.27 -1.1 515,870 - PGA1_c05220 0.28 -1.8 515,882 + PGA1_c05220 0.29 -1.0 515,887 + PGA1_c05220 0.30 -0.9 515,887 + PGA1_c05220 0.30 -1.1 515,887 + PGA1_c05220 0.30 +2.2 515,887 + PGA1_c05220 0.30 +2.3 515,887 + PGA1_c05220 0.30 -1.7 515,887 + PGA1_c05220 0.30 +1.6 515,895 - PGA1_c05220 0.30 +3.4 515,895 - PGA1_c05220 0.30 -0.5 515,948 + PGA1_c05220 0.35 +1.2 515,953 + PGA1_c05220 0.35 -2.9 515,956 - PGA1_c05220 0.36 +2.0 516,004 + PGA1_c05220 0.40 -2.3 516,010 + PGA1_c05220 0.41 -2.8 516,018 - PGA1_c05220 0.41 +1.5 516,039 - PGA1_c05220 0.43 -0.6 516,077 + PGA1_c05220 0.47 -1.9 516,204 - PGA1_c05220 0.58 +1.9 516,209 - PGA1_c05220 0.58 -1.8 516,209 - PGA1_c05220 0.58 -0.7 516,219 + PGA1_c05220 0.59 +1.4 516,219 + PGA1_c05220 0.59 -1.7 516,257 + PGA1_c05220 0.63 -1.8 516,372 - PGA1_c05220 0.73 +3.1 516,431 + PGA1_c05220 0.78 -0.4 516,441 - PGA1_c05220 0.79 +1.1 516,587 + +2.8 516,592 + -1.5 516,711 - -2.6 516,751 + +1.8 516,768 + -1.4 516,783 + -1.3 516,783 - -2.1 516,791 - -1.5 516,815 + +1.9 516,829 + +2.3 516,837 - -1.4 516,945 - -2.3 516,958 - -1.2 516,962 + PGA1_c05230 0.10 -1.9 516,969 + PGA1_c05230 0.11 +3.5 516,970 - PGA1_c05230 0.11 -1.5 517,002 + PGA1_c05230 0.12 +1.0 517,002 + PGA1_c05230 0.12 -0.6 517,033 + PGA1_c05230 0.14 +3.4 517,041 - PGA1_c05230 0.15 +2.2 517,060 - PGA1_c05230 0.16 +2.9 517,070 - PGA1_c05230 0.16 -2.3 517,073 + PGA1_c05230 0.17 +1.3 517,073 + PGA1_c05230 0.17 -1.7 517,073 + PGA1_c05230 0.17 +0.6 517,091 + PGA1_c05230 0.18 +0.0 517,110 - PGA1_c05230 0.19 -1.1 517,173 + PGA1_c05230 0.22 +2.4 517,180 - PGA1_c05230 0.23 -0.6 517,198 - PGA1_c05230 0.24 +2.0 517,212 + PGA1_c05230 0.25 +2.7 517,212 + PGA1_c05230 0.25 -1.6 517,220 - PGA1_c05230 0.25 +2.8 517,246 + PGA1_c05230 0.27 -1.0 517,246 + PGA1_c05230 0.27 -2.8 517,246 + PGA1_c05230 0.27 -1.0 517,246 + PGA1_c05230 0.27 -1.8 517,254 - PGA1_c05230 0.27 -2.9 517,254 - PGA1_c05230 0.27 +2.1 517,254 - PGA1_c05230 0.27 +1.7 517,254 - PGA1_c05230 0.27 +2.3 517,254 - PGA1_c05230 0.27 +0.5 517,256 - PGA1_c05230 0.27 -0.1 517,318 + PGA1_c05230 0.31 +2.6 517,365 + PGA1_c05230 0.33 +1.7 517,378 - PGA1_c05230 0.34 +1.2 517,503 - PGA1_c05230 0.41 +3.3 517,505 + PGA1_c05230 0.41 +1.2 517,505 + PGA1_c05230 0.41 -2.9 517,513 - PGA1_c05230 0.42 +2.1 517,590 - PGA1_c05230 0.46 +1.5 517,761 - PGA1_c05230 0.56 +2.8 517,769 + PGA1_c05230 0.57 +2.8 517,826 + PGA1_c05230 0.60 -1.9 517,857 + PGA1_c05230 0.62 +4.2 517,878 + PGA1_c05230 0.63 +1.3 517,878 - PGA1_c05230 0.63 +0.7 517,886 + PGA1_c05230 0.63 -1.9 517,900 - PGA1_c05230 0.64 -0.2 517,931 - PGA1_c05230 0.66 +1.5 517,931 - PGA1_c05230 0.66 -1.5 517,960 + PGA1_c05230 0.68 +3.2 517,960 + PGA1_c05230 0.68 +0.3 517,968 - PGA1_c05230 0.68 -0.5 518,017 + PGA1_c05230 0.71 -2.3 518,017 + PGA1_c05230 0.71 +3.4 518,017 + PGA1_c05230 0.71 -2.1 518,017 + PGA1_c05230 0.71 +0.3 518,017 + PGA1_c05230 0.71 -1.9 518,025 - PGA1_c05230 0.71 +1.1 518,025 - PGA1_c05230 0.71 -1.2 518,025 - PGA1_c05230 0.71 +1.4 518,025 - PGA1_c05230 0.71 +2.2 518,025 - PGA1_c05230 0.71 -1.6 518,025 - PGA1_c05230 0.71 +1.2 518,025 - PGA1_c05230 0.71 +2.0 518,032 + PGA1_c05230 0.72 -1.9 518,040 - PGA1_c05230 0.72 +2.7 518,072 - PGA1_c05230 0.74 -0.1 518,097 + PGA1_c05230 0.76 +3.2 518,131 + PGA1_c05230 0.78 +1.8 518,139 - PGA1_c05230 0.78 -2.5 518,149 - PGA1_c05230 0.79 -1.1 518,149 - PGA1_c05230 0.79 +2.4 518,186 + PGA1_c05230 0.81 -1.0 518,193 + PGA1_c05230 0.81 +1.7 518,193 + PGA1_c05230 0.81 +1.4 518,193 + PGA1_c05230 0.81 +0.8 518,201 - PGA1_c05230 0.82 -0.6 518,201 - PGA1_c05230 0.82 +0.3 518,201 - PGA1_c05230 0.82 +2.4 518,201 - PGA1_c05230 0.82 +2.5 518,201 - PGA1_c05230 0.82 +0.9 518,211 - PGA1_c05230 0.82 -0.9 518,211 - PGA1_c05230 0.82 +1.7 518,211 - PGA1_c05230 0.82 +1.8 518,229 + PGA1_c05230 0.83 +2.6 518,243 + PGA1_c05230 0.84 -1.0 518,274 + PGA1_c05230 0.86 -1.5 518,274 + PGA1_c05230 0.86 -0.5 518,274 + PGA1_c05230 0.86 -0.1 518,311 + PGA1_c05230 0.88 -2.6 518,311 + PGA1_c05230 0.88 -2.2 518,311 + PGA1_c05230 0.88 +2.1 518,338 + PGA1_c05230 0.90 +0.9 518,338 + PGA1_c05230 0.90 -0.1 518,338 + PGA1_c05230 0.90 -0.8 518,338 + PGA1_c05230 0.90 -2.3 518,338 + PGA1_c05230 0.90 +0.8 518,346 - -0.0 518,346 - +2.4 518,346 - +1.9 518,346 - -1.8 518,346 - +3.8 518,346 - +0.4 518,348 + +2.7 518,355 - +4.1 518,356 - -0.0 518,356 - +1.3 518,397 + +3.4 518,397 + +2.9 518,397 + +1.8 518,400 - -1.2 518,454 + -1.7 518,458 - +2.8 518,462 - +1.9 518,512 - +1.8 518,536 + -2.0 518,536 + -1.1 518,544 - -0.7 518,583 + +0.4 518,591 - -1.3 518,680 - -2.2 518,693 + +0.6 518,694 - +3.8 518,701 - +0.2 518,731 + -1.9 518,803 + -1.6 518,838 + +2.8 518,898 + -1.7 518,967 + PGA1_c05240 0.11 -1.3 518,976 - PGA1_c05240 0.11 +2.2 519,024 + PGA1_c05240 0.16 +1.0 519,041 + PGA1_c05240 0.17 +2.4 519,058 + PGA1_c05240 0.19 -1.6 519,058 + PGA1_c05240 0.19 -0.5 519,066 - PGA1_c05240 0.19 +0.2 519,113 + PGA1_c05240 0.24 +4.8 519,113 + PGA1_c05240 0.24 +2.1 519,118 + PGA1_c05240 0.24 -0.4 519,134 - PGA1_c05240 0.25 -2.0 519,206 + PGA1_c05240 0.32 +2.0 519,206 + PGA1_c05240 0.32 -2.3 519,214 - PGA1_c05240 0.32 -2.7 519,214 - PGA1_c05240 0.32 -2.0 519,233 + PGA1_c05240 0.34 +4.2 519,256 + PGA1_c05240 0.36 +1.1 519,264 - PGA1_c05240 0.37 +2.8 519,264 - PGA1_c05240 0.37 +1.5 519,264 - PGA1_c05240 0.37 +0.8 519,264 - PGA1_c05240 0.37 -2.2 519,273 + PGA1_c05240 0.38 +2.4 519,306 - PGA1_c05240 0.41 -2.7 519,328 - PGA1_c05240 0.43 +4.3 519,342 + PGA1_c05240 0.44 -1.8 519,347 + PGA1_c05240 0.44 +2.5 519,434 - PGA1_c05240 0.52 +1.9 519,455 + PGA1_c05240 0.54 +1.4
Or see this region's nucleotide sequence